Q: How do we manually annotate a DNAtraffic entry?

A: A well-defined manual curation process is essential to ensure that all manually annotated entries are handled in a consistent manner. Curation is performed by expert biologists and chemists using a range of tools that have been iteratively developed in close collaboration with curators.

Q: Where do the DNAtraffic protein and gene sequences come from?

A: The protein and gene sequences provided by DNAtraffic database come from the UniProt and KEGG databases, respectively.

Q: Why do I find many cross-references to PDB in DNAtraffic database?

A: Some proteins are highly popular because they are of medical interest or can be used in biotechnology and systems bilogy study. As a consequence, there is more than one cross-reference to PDB because a number of different structures are available for some proteins. 3D structures convey important experimental evidence on a protein: its secondary structure, how it is folded, which parts of the polypeptide chain are available for interactions with other proteins, which residues are directly involved in the catalytic activity, in metal-binding, etc.

Because 3D structures are so important, we endeavour to be as up-to-date as possible and to show all available cross-references to PDB from proteins in our knowledgebase.

Q: What data resources are included in DNAtraffic DB?