Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA-directed RNA polymerase II subunit RPB7


DESCRIPTION:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA. The RPB4-RPB7 subcomplex recruits FCP1 to Pol II.

MISCELLANEOUS:
Present with 6420 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.


RELATED PATHWAY(S):
transcription


Amino acids sequence

        10         20         30         40         50         60
MFFIKDLSLN ITLHPSFFGP RMKQYLKTKL LEEVEGSCTG KFGYILCVLD YDNIDIQRGR
        70         80         90        100        110        120
ILPTDGSAEF NVKYRAVVFK PFKGEVVDGT VVSCSQHGFE VQVGPMKVFV TKHLMPQDLT
       130        140        150        160        170
FNAGSNPPSY QSSEDVITIK SRIRVKIEGC ISQVSSIHAI GSIKEDYLGA I

Encoded by RPB7 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtttttta ttaaagacct ttcgcttaat attacccttc atccgtcctt tttcggtcct
        70         80         90        100        110        120
cgaatgaagc aatatctaaa gacaaaacta ttggaagagg ttgaaggttc gtgtacgggt
       130        140        150        160        170        180
aaattcggat atattctttg tgtcctagac tatgataata tagatattca acgtgggaga
       190        200        210        220        230        240
atattgccca cagatgggtc cgccgagttc aacgtgaaat atagagctgt agttttcaaa
       250        260        270        280        290        300
ccatttaaag gggaagtagt ggacggcaca gtcgtttcat gttctcagca cgggttcgaa
       310        320        330        340        350        360
gtgcaagtag gtccaatgaa agtatttgtg acaaagcatc tgatgcctca agatttaacc
       370        380        390        400        410        420
tttaatgcgg gttcaaaccc accatcatac caaagttccg aggatgtcat caccataaaa
       430        440        450        460        470        480
agtagaatta gagttaaaat tgaaggttgt atcagtcaag tgagttctat tcacgcaatc
       490        500        510
ggtagtatca aagaagatta tttgggtgct atttaa  

Last modification date: Oct. 2, 2011