Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: DNA polymerase alpha catalytic subunit


DESCRIPTION:
Polymerase alpha in a complex with DNA primase is a replicative polymerase.

MISCELLANEOUS:
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.


STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-B family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MRKRNAGIKP SQRFAELRAL RQAGKTRAST YESKENEELY DNVSEEEYRK IVRQRLDEDD
        70         80         90        100        110        120
FVVDDNGAGY VDNGYDEWDQ SHYSDEDDEN EKGSSGRKRK KSKNVLAEPN QQIGAFFKAN
       130        140        150        160        170        180
AIQPASRIAN RKNNEKEDEF MAEILGSIDQ DIPERLSTKK GNRSHTTSNA KRRSQKAASS
       190        200        210        220        230        240
ISTTNVVSSD PKKYIPETVP STPQPASSLP IPSSPPAALE TEENVDSQPM ELDEPELPVG
       250        260        270        280        290        300
SDAPIIDVEE SIAMKPLMSH DQVDDTASLS KATISQQPLS PMTPLSSELD VSAFSEINSK
       310        320        330        340        350        360
IKNVDVPASS YSSPISKVSP SDVTEEDGSL FFFWMDYTEM YGSLCLFGKV YDKATKQYVS
       370        380        390        400        410        420
CFLKVDGIMR SLYFLPRPSS SSVSEDSIAA QTKDVYDEVA NLLSKRGVKE WKSRVSKYKY
       430        440        450        460        470        480
AFELEDVPRT ADYLEVIYSY SYPALPTDLT GSSFSHVFGT NTALFEQFVL SRRVMGPCWL
       490        500        510        520        530        540
KIQQPNFDAV KNASWCRVEI GCSSPQNISV SFEKNEITSK TPPMTVMSLA FRTLINKEQN
       550        560        570        580        590        600
KQEVVMISAR IFENVDIEKG LPANDMPSYS FSLIRPLKQI FPNGFEKLAR QHKSSIFCER
       610        620        630        640        650        660
SEVSLLNNFL NKVRTYDPDV YFGHDFEMCY SVLLSRLKER KIHNWSSIGR LRRSEWPRSF
       670        680        690        700        710        720
NRSSQQFVEK QIIAGRLMCD LSNDFGRSMI KAQSWSLSEI VLKELDIKRQ DINQEKALQS
       730        740        750        760        770        780
WTDTAHGLLD YLVHCEIDTF FIAAVAFKIQ MLQLSKNLTN IAGNSWARTL TGTRAERNEY
       790        800        810        820        830        840
ILLHEFKKNG YIVPDKQQSI RRHAEAFGAE DGLQEESLGK KKDKYKGGLV FEPQKGLYET
       850        860        870        880        890        900
CILVMDFNSL YPSIIQEYNI CFTTVDRSPS NSDSDDQIPD TPSASANQGI FPRLIANLVE
       910        920        930        940        950        960
RRRQIKGLLK DNSATPTQRL QWDIQQQALK LTANSMYGCL GYTKSRFYAR PLAVLITYKG
       970        980        990       1000       1010       1020
REALMNTKEL ADQMGLQVIY GDTDSVMLNT NVTDKNHALR IGNEFKEKVN ERYSKLEIDI
      1030       1040       1050       1060       1070       1080
DNVYQRMLLH AKKKYAALQL DSQGKPNLDV KGLDMKRREF CTLAKEASKF CLDQILSGEL
      1090       1100       1110       1120       1130       1140
TETVIENIHS YLMDFSEKMR NGKFPANKFI IFNRLGKNPE DYPNGKTMPF VQVALKKKAR
      1150       1160       1170       1180       1190       1200
GENVRVGDVI PFIIAGSDAD GHPADRAYSP QEIMNTNSTL VIDYNYYLSH QILPPIERVI
      1210       1220       1230       1240       1250       1260
APIEGTNRAR LAECLGLDAR KYYSHETSES SAFQRYESTL TDDQCFINVS PLLLKCPSCN
      1270       1280       1290       1300       1310       1320
ATSFSLRSVK SLKETLYANT VECDCGYEYS DFTIILQFSS QLRDFINLYY EGILVCDDSS
      1330       1340       1350       1360       1370       1380
CGNRTRQMSV YGKRCCNKSC RGSVHFEYND EQLYNQIKFL LKAVQTTTGA TRNGIIRCNA
      1390       1400
INKNISRIMN KNAREFVDMG LIFSS   

Encoded by pol1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgagaaaga gaaacgcggg gataaagccg tctcaaagat ttgctgagtt acgggctctt
        70         80         90        100        110        120
cgtcaagccg gaaaaacaag ggctagcaca tacgaaagca aagaaaatga agaattatat
       130        140        150        160        170        180
gacaatgttt cagaggaaga atatcgcaag attgttcgac aacgactgga tgaggatgac
       190        200        210        220        230        240
tttgtcgtag acgataacgg ggctggttat gtggataatg gatatgatga gtgggatcaa
       250        260        270        280        290        300
agtcattaca gtgacgagga tgatgaaaat gaaaaaggat cgtctggtcg aaaaagaaag
       310        320        330        340        350        360
aaatcgaaga acgttctagc tgaacctaat caacaaattg gcgcgttttt taaagcaaat
       370        380        390        400        410        420
gctatccaac ctgcttcacg tatcgcaaat cgaaaaaata atgagaagga agatgaattc
       430        440        450        460        470        480
atggctgaaa ttcttggctc aatcgaccaa gatattcccg aacgcctctc gacaaaaaag
       490        500        510        520        530        540
ggaaatcgtt ctcatactac atcaaatgcc aaacgtcgct ctcagaaagc tgcttcctca
       550        560        570        580        590        600
ataagtacaa ctaacgttgt ttcttctgat cccaaaaaat acatcccaga aactgtacct
       610        620        630        640        650        660
tcaactcctc aacctgctag cagtcttcct attcccagct ccccacctgc tgcactcgag
       670        680        690        700        710        720
acagaagaaa atgtcgacag tcaaccaatg gaattagacg aaccagaact ccccgttgga
       730        740        750        760        770        780
tctgatgcac ccattattga cgttgaagaa tctattgcca tgaagccatt gatgagtcat
       790        800        810        820        830        840
gatcaggtcg atgacaccgc atcgctttca aaagctacta tttctcagca acctctatca
       850        860        870        880        890        900
ccaatgacac ctttatcttc ggaacttgac gtctctgctt tttctgaaat taattctaag
       910        920        930        940        950        960
ataaagaatg tagatgttcc tgcttcgtcc tattcatctc caatttcaaa agtatcacca
       970        980        990       1000       1010       1020
tctgatgtta ctgaagagga tggttctttg tttttttttt ggatggatta taccgagatg
      1030       1040       1050       1060       1070       1080
tacggctcat tgtgtctatt tggcaaagta tatgataaag ctactaaaca gtatgtttcg
      1090       1100       1110       1120       1130       1140
tgctttctca aggtcgatgg gataatgcgt tctttatact tccttccccg cccatcatca
      1150       1160       1170       1180       1190       1200
tcctcagttt ctgaagattc tattgcagcg caaactaaag atgtttacga cgaagtagcc
      1210       1220       1230       1240       1250       1260
aatttgttat ctaaaagggg tgtcaaggaa tggaaatcaa gagtctccaa gtataaatat
      1270       1280       1290       1300       1310       1320
gctttcgaat tagaagatgt tcctcggacc gctgattact tagaggtaat ttattcctat
      1330       1340       1350       1360       1370       1380
tcttatcctg ctctgcctac tgatttgact ggttcttctt tctcacacgt ttttggaact
      1390       1400       1410       1420       1430       1440
aataccgcct tattcgagca gtttgtctta agtagaagag ttatgggtcc ttgttggctg
      1450       1460       1470       1480       1490       1500
aaaatacaac aaccaaattt tgatgctgta aaaaatgcta gttggtgccg ggtcgaaata
      1510       1520       1530       1540       1550       1560
ggatgttcgt cacctcagaa catttctgtt tctttcgaaa aaaatgaaat tacttcgaaa
      1570       1580       1590       1600       1610       1620
acgcctccaa tgacggtgat gagtcttgca tttagaacgc tgattaacaa agagcaaaac
      1630       1640       1650       1660       1670       1680
aagcaagaag tcgttatgat tagtgctagg atttttgaaa atgttgacat tgaaaaaggt
      1690       1700       1710       1720       1730       1740
ttgcctgcaa atgacatgcc ctcgtattcc ttttcgttga ttcgtccttt gaagcaaatc
      1750       1760       1770       1780       1790       1800
tttccaaatg gttttgaaaa gctagctcgc cagcacaaat cttccatctt ttgtgagagg
      1810       1820       1830       1840       1850       1860
tctgaagttt cgttgcttaa taatttctta aataaagttc gtacatacga tcctgatgtt
      1870       1880       1890       1900       1910       1920
tattttgggc atgattttga aatgtgctac tcagtattgt tatctagact gaaagaacga
      1930       1940       1950       1960       1970       1980
aaaattcata attggagtag tattggtcga cttcgaagaa gcgaatggcc acgttcgttt
      1990       2000       2010       2020       2030       2040
aatagaagct ctcaacagtt cgtagaaaaa cagattattg ctggaaggct tatgtgtgat
      2050       2060       2070       2080       2090       2100
ttatctaacg actttggtcg atctatgatt aaagcccaat cttggagttt atctgaaata
      2110       2120       2130       2140       2150       2160
gtacttaagg agcttgacat taaacgtcaa gacattaatc aagagaaggc attgcaatca
      2170       2180       2190       2200       2210       2220
tggaccgata cggctcacgg cttattggat tatttagttc actgtgaaat tgacacattc
      2230       2240       2250       2260       2270       2280
tttattgctg ccgtcgcatt caaaattcag atgcttcagc tatcaaagaa tttaacaaac
      2290       2300       2310       2320       2330       2340
attgctggta actcttgggc tagaacatta acaggtacca gagcggaaag aaacgaatac
      2350       2360       2370       2380       2390       2400
attcttcttc acgaatttaa aaaaaatgga tatattgtcc cggataaaca acaaagtatt
      2410       2420       2430       2440       2450       2460
agaagacatg ctgaagcttt tggtgcagaa gacggtctgc aagaagaatc tcttggcaag
      2470       2480       2490       2500       2510       2520
aaaaaagata aatacaaagg tggattggtt tttgagcctc aaaaaggact ttatgaaact
      2530       2540       2550       2560       2570       2580
tgtattttgg tgatggactt caatagttta tatccaagta ttatccaaga atacaatatt
      2590       2600       2610       2620       2630       2640
tgttttacta ctgttgacag atctccttct aactctgatt ccgacgatca aattcccgat
      2650       2660       2670       2680       2690       2700
actccatctg cctcggccaa tcaaggtata tttcctcgat taattgcaaa tcttgtggaa
      2710       2720       2730       2740       2750       2760
cgtcgacgcc aaattaaagg actattaaag gataactctg ccactcccac acaaaggctg
      2770       2780       2790       2800       2810       2820
caatgggata tccaacaaca agccctgaag ttaactgcaa actccatgta tggctgcctg
      2830       2840       2850       2860       2870       2880
ggatatacga agtctcgatt ctatgcacgt ccgttggctg ttctgattac ttataagggt
      2890       2900       2910       2920       2930       2940
agggaagcct tgatgaatac taaagaactt gcggatcaaa tgggacttca agtaatttat
      2950       2960       2970       2980       2990       3000
ggcgataccg attcagttat gctcaatacg aacgtaacag acaagaacca tgctcttcga
      3010       3020       3030       3040       3050       3060
attggtaatg aatttaagga aaaggtaaat gaacgttata gtaaactaga aattgatatc
      3070       3080       3090       3100       3110       3120
gataacgttt atcagcgcat gcttctgcat gctaagaaaa agtacgcggc tttacagctt
      3130       3140       3150       3160       3170       3180
gattctcaag ggaagcccaa tttagatgta aagggcttgg atatgaaacg tcgtgagttt
      3190       3200       3210       3220       3230       3240
tgtactttag ctaaggaggc tagtaaattt tgtttggatc aaattttatc tggtgaatta
      3250       3260       3270       3280       3290       3300
acggaaaccg taattgaaaa tattcattct tatttaatgg atttttccga aaaaatgcgt
      3310       3320       3330       3340       3350       3360
aatgggaagt ttccggctaa caaatttata atattcaatc gtttgggaaa aaatccagaa
      3370       3380       3390       3400       3410       3420
gattatccaa atggaaagac gatgccattc gtgcaggtgg ctcttaaaaa gaaagctaga
      3430       3440       3450       3460       3470       3480
ggagagaatg ttcgtgtagg cgatgttatt cctttcatta ttgctggtag tgacgctgac
      3490       3500       3510       3520       3530       3540
ggacaccctg ccgaccgagc ttatagtccg caagaaataa tgaatacaaa ttccacatta
      3550       3560       3570       3580       3590       3600
gttattgact acaattatta tctttcgcat caaattctcc ctcctatcga gcgtgttata
      3610       3620       3630       3640       3650       3660
gcaccgattg aaggaacaaa ccgtgctcgc ttagctgaat gcttaggttt ggatgctcga
      3670       3680       3690       3700       3710       3720
aaatactact cacatgagac ttctgaatca tctgcgtttc agcggtatga gtctacgctc
      3730       3740       3750       3760       3770       3780
actgatgacc agtgttttat taacgtttcc ccattactat tgaaatgtcc atcctgtaat
      3790       3800       3810       3820       3830       3840
gctacatcat tttccctacg atctgtaaag tccttgaagg aaacgttgta tgcgaataca
      3850       3860       3870       3880       3890       3900
gtagaatgtg attgtggata tgagtattcg gactttacta ttattcttca attctcatct
      3910       3920       3930       3940       3950       3960
caactcagag attttattaa tctttattat gaaggaattt tagtatgtga tgattcttct
      3970       3980       3990       4000       4010       4020
tgtggtaatc gaacacgaca aatgagtgtg tatggaaagc gttgttgtaa caaatcatgt
      4030       4040       4050       4060       4070       4080
cgtggaagcg ttcattttga atataatgat gaacaattat ataaccagat taagttcctg
      4090       4100       4110       4120       4130       4140
ctaaaagctg ttcaaacaac caccggggct accagaaatg gaatcattag atgcaatgcc
      4150       4160       4170       4180       4190       4200
atcaacaaaa atatctctcg aataatgaac aaaaatgcgc gtgaatttgt agatatggga
      4210
ctgatatttt catcgtga    

Last modification date: Oct. 2, 2011