Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA ligase 1


DESCRIPTION:
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. The mitochondrial form is required for mitochondrial DNA maintenance but is non-essential while the nuclear form is essential for cell viability.

MISCELLANEOUS:
Present with 149 molecules/cell in log phase SD medium.
Cdc9 is included within the category of so-called 'start genes', encoding proteins which are required in early G1, when the cell is faced with the option of initiating a further cell cycle.


STRUCTURE SIMILARITY:
Belongs to the ATP-dependent DNA ligase family.


CATALYTIC ACTIVITY:
ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MRRLLTGCLL SSARPLKSRL PLLMSSSLPS SAGKKPKQAT LARFFTSMKN KPTEGTPSPK
        70         80         90        100        110        120
KSSKHMLEDR MDNVSGEEEY ATKKLKQTAV THTVAAPSSM GSNFSSIPSS APSSGVADSP
       130        140        150        160        170        180
QQSQRLVGEV EDALSSNNND HYSSNIPYSE VCEVFNKIEA ISSRLEIIRI CSDFFIKIMK
       190        200        210        220        230        240
QSSKNLIPTT YLFINRLGPD YEAGLELGLG ENLLMKTISE TCGKSMSQIK LKYKDIGDLG
       250        260        270        280        290        300
EIAMGARNVQ PTMFKPKPLT VGEVFKNLRA IAKTQGKDSQ LKKMKLIKRM LTACKGIEAK
       310        320        330        340        350        360
FLIRSLESKL RIGLAEKTVL ISLSKALLLH DENREDSPDK DVPMDVLESA QQKIRDAFCQ
       370        380        390        400        410        420
VPNYEIVINS CLEHGIMNLD KYCTLRPGIP LKPMLAKPTK AINEVLDRFQ GETFTSEYKY
       430        440        450        460        470        480
DGERAQVHLL NDGTMRIYSR NGENMTERYP EINITDFIQD LDTTKNLILD CEAVAWDKDQ
       490        500        510        520        530        540
GKILPFQVLS TRKRKDVELN DVKVKVCLFA FDILCYNDER LINKSLKERR EYLTKVTKVV
       550        560        570        580        590        600
PGEFQYATQI TTNNLDELQK FLDESVNHSC EGLMVKMLEG PESHYEPSKR SRNWLKLKKD
       610        620        630        640        650        660
YLEGVGDSLD LCVLGAYYGR GKRTGTYGGF LLGCYNQDTG EFETCCKIGT GFSDEMLQLL
       670        680        690        700        710        720
HDRLTPTIID GPKATFVFDS SAEPDVWFEP TTLFEVLTAD LSLSPIYKAG SATFDKGVSL
       730        740        750
RFPRFLRIRE DKGVEDATSS DQIVELYENQ SHMQN  

Encoded by CDC9 gene


OTHER NAME(S):
MMS8


Nucleic acid sequence

        10         20         30         40         50         60
atgcgcagat tactgaccgg ttgccttctt tcatctgcac gtcccttgaa atcaagattg
        70         80         90        100        110        120
ccattattga tgtcatcctc attaccttcc tctgccggta agaagcctaa acaagccact
       130        140        150        160        170        180
ttggctagat tcttcacttc catgaaaaat aagccaacag aaggtactcc ttctcccaaa
       190        200        210        220        230        240
aaatcatcca aacatatgct ggaagataga atggataatg ttagtgggga agaggaatac
       250        260        270        280        290        300
gcgaccaaga aattgaaaca gacggctgtt acacatactg tagcagctcc aagctccatg
       310        320        330        340        350        360
ggtagcaatt tttcttctat accatcatcc gctccctctt ccggtgttgc tgattcaccg
       370        380        390        400        410        420
caacaatctc agaggttggt aggtgaagtt gaagacgcct tgagctcaaa taataatgat
       430        440        450        460        470        480
cactattcgt ctaatatccc ctattctgaa gtttgtgagg tttttaacaa gattgaggcc
       490        500        510        520        530        540
atatcttccc gtttagagat tataagaatc tgttctgatt tctttattaa gataatgaag
       550        560        570        580        590        600
caatcgtcta agaacttaat acctacaaca taccttttca tcaatagatt gggccctgac
       610        620        630        640        650        660
tacgaagcag gcttggaatt aggccttgga gaaaatcttc ttatgaaaac cataagcgaa
       670        680        690        700        710        720
acttgcggga aatccatgag ccaaattaaa cttaaataca aggatattgg ggatttgggt
       730        740        750        760        770        780
gaaatagcaa tgggtgcgag aaatgtccaa cccactatgt ttaagcccaa acccttgacc
       790        800        810        820        830        840
gtgggtgagg tcttcaaaaa tcttagagct attgctaaga ctcagggcaa agattcacaa
       850        860        870        880        890        900
ttaaaaaaga tgaagctgat caagagaatg cttactgctt gcaaaggtat agaggctaag
       910        920        930        940        950        960
tttttgatta gatccctaga gtcaaaattg agaatcggtc ttgctgaaaa gactgtctta
       970        980        990       1000       1010       1020
atatccctat cgaaggctct tttgctccat gatgaaaata gggaggactc tccagacaaa
      1030       1040       1050       1060       1070       1080
gatgtcccca tggatgtgtt agaaagtgct caacaaaaga taagagacgc attttgtcaa
      1090       1100       1110       1120       1130       1140
gtacccaact atgaaattgt tataaactca tgcctggaac acggtattat gaatttagat
      1150       1160       1170       1180       1190       1200
aaatattgta ctctaagacc aggaattcca ttgaaaccca tgttagccaa gcctaccaag
      1210       1220       1230       1240       1250       1260
gcaattaatg aagtattgga tagatttcaa ggcgaaactt ttacgtcaga atacaaatac
      1270       1280       1290       1300       1310       1320
gatggtgaaa gggctcaggt gcatttacta aatgatggta caatgaggat ttattccaga
      1330       1340       1350       1360       1370       1380
aatggcgaga atatgactga gagatatcca gaaattaaca taacggattt tatccaggat
      1390       1400       1410       1420       1430       1440
ttggatacta ccaaaaattt aattctagac tgtgaggctg ttgcttggga caaagatcag
      1450       1460       1470       1480       1490       1500
gggaaaattt tgcctttcca ggtcttaagt acaaggaaac gtaaagacgt ggagctgaat
      1510       1520       1530       1540       1550       1560
gatgttaaag ttaaagtctg tctctttgca tttgatatac tttgctacaa cgatgaaagg
      1570       1580       1590       1600       1610       1620
ctgattaata agtcactaaa ggaaagaaga gaatacttga cgaaggttac aaaagttgtc
      1630       1640       1650       1660       1670       1680
ccgggagaat tccaatacgc gactcagata acaacaaata accttgatga acttcaaaaa
      1690       1700       1710       1720       1730       1740
ttccttgatg agtccgttaa tcactcatgc gaaggtttga tggtcaaaat gttggagggc
      1750       1760       1770       1780       1790       1800
cctgaatccc attatgaacc aagtaaaagg tcaagaaact ggttgaaatt aaaaaaagat
      1810       1820       1830       1840       1850       1860
tacttggagg gggttggaga ttcattggat ctctgcgtgt tgggggcata ttatggtaga
      1870       1880       1890       1900       1910       1920
ggaaaaagaa ctggtacgta cggtgggttc cttctaggat gttacaacca agacacagga
      1930       1940       1950       1960       1970       1980
gaatttgaaa cttgttgcaa gatagggaca ggattttcgg acgaaatgtt gcagcttttg
      1990       2000       2010       2020       2030       2040
cacgacaggt tgaccccgac gatcattgat ggaccaaaag ccacgtttgt tttcgattcg
      2050       2060       2070       2080       2090       2100
agtgctgagc cagatgtatg gttcgaacct acgactttgt ttgaagtttt gactgcagac
      2110       2120       2130       2140       2150       2160
ctttccctat cgcctattta taaagcaggt agtgcaactt ttgataaagg cgtatcctta
      2170       2180       2190       2200       2210       2220
agatttccaa gatttttgcg tataagagaa gacaagggcg tagaagatgc aacctcttct
      2230       2240       2250       2260
gaccagatcg tcgaattgta tgaaaaccaa tcccacatgc aaaattag 

Last modification date: Oct. 2, 2011