Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Flap endonuclease 1


DESCRIPTION:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

MISCELLANEOUS:
Present with 6120 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma (By similarity).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
non-homologous end-joining (NHEJ)


Amino acids sequence

        10         20         30         40         50         60
MGIKGLNAII SEHVPSAIRK SDIKSFFGRK VAIDASMSLY QFLIAVRQQD GGQLTNEAGE
        70         80         90        100        110        120
TTSHLMGMFY RTLRMIDNGI KPCYVFDGKP PDLKSHELTK RSSRRVETEK KLAEATTELE
       130        140        150        160        170        180
KMKQERRLVK VSKEHNEEAQ KLLGLMGIPY IIAPTEAEAQ CAELAKKGKV YAAASEDMDT
       190        200        210        220        230        240
LCYRTPFLLR HLTFSEAKKE PIHEIDTELV LRGLDLTIEQ FVDLCIMLGC DYCESIRGVG
       250        260        270        280        290        300
PVTALKLIKT HGSIEKIVEF IESGESNNTK WKIPEDWPYK QARMLFLDPE VIDGNEINLK
       310        320        330        340        350        360
WSPPKEKELI EYLCDDKKFS EERVKSGISR LKKGLKSGIQ GRLDGFFQVV PKTKEQLAAA
       370        380
AKRAQENKKL NKNKNKVTKG RR   

Encoded by RAD27 gene


OTHER NAME(S):
ERC11
FEN1
RTH1


Nucleic acid sequence

        10         20         30         40         50         60
atgggtatta aaggtttgaa tgcaattata tcggaacatg ttccctctgc tatcaggaaa
        70         80         90        100        110        120
agcgatatca agagcttttt tggcagaaag gttgccatcg atgcctctat gtctctatat
       130        140        150        160        170        180
cagtttttaa ttgctgtaag acagcaagac ggtgggcagt tgaccaatga agccggtgaa
       190        200        210        220        230        240
acaacgtcac acttgatggg tatgttttat aggacactga gaatgattga taacggtatc
       250        260        270        280        290        300
aagccttgtt atgtcttcga cggcaaacct ccagatttga aatctcatga gttgacaaag
       310        320        330        340        350        360
cggtcttcaa gaagggtgga aacagaaaaa aaactggcag aggcaacaac agaattggaa
       370        380        390        400        410        420
aagatgaagc aagaaagaag attggtgaag gtctcaaaag agcataatga agaagcccaa
       430        440        450        460        470        480
aaattactag gactaatggg aatcccatat ataatagcgc caacggaagc tgaggctcaa
       490        500        510        520        530        540
tgtgctgagt tggcaaagaa gggaaaggtg tatgccgcag caagtgaaga tatggacaca
       550        560        570        580        590        600
ctctgttata gaacaccctt cttgttgaga catttgactt tttcagaggc caagaaggaa
       610        620        630        640        650        660
ccgattcacg aaatagatac tgaattagtt ttgagaggac tcgacttgac aatagagcag
       670        680        690        700        710        720
tttgttgatc tttgcataat gcttggttgt gactactgtg aaagcatcag aggtgttggt
       730        740        750        760        770        780
ccagtgacag ccttaaaatt gataaaaacg catggatcca tcgaaaaaat cgtggagttt
       790        800        810        820        830        840
attgaatctg gggagtcaaa caacactaaa tggaaaatcc cagaagactg gccttacaaa
       850        860        870        880        890        900
caagcaagaa tgctgtttct tgaccctgaa gttatagatg gtaacgaaat aaacttgaaa
       910        920        930        940        950        960
tggtcgccac caaaggagaa ggaacttatc gagtatttat gtgatgataa gaaattcagt
       970        980        990       1000       1010       1020
gaagaaagag ttaaatctgg tatatcaaga ttgaaaaaag gcttgaaatc tggcattcag
      1030       1040       1050       1060       1070       1080
ggtaggttag atgggttctt ccaagtggtg cctaagacaa aggaacagct ggctgctgcg
      1090       1100       1110       1120       1130       1140
gcgaaaagag cacaagaaaa taaaaaattg aacaaaaata agaataaagt cacaaaggga

agaagatga     

Last modification date: Oct. 2, 2011