Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Ribonuclease H2 subunit A


DESCRIPTION:
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

MISCELLANEOUS:
Present with 623 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the RNase HII family. Eukaryotic subfamily.


CATALYTIC ACTIVITY:
Endonucleolytic cleavage to 5'-phosphomonoester.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MVPPTVEASL ESPYTKSYFS PVPSALLEQN DSPIIMGIDE AGRGPVLGPM VYAVAYSTQK
        70         80         90        100        110        120
YQDETIIPNY EFDDSKKLTD PIRRMLFSKI YQDNEELTQI GYATTCITPL DISRGMSKFP
       130        140        150        160        170        180
PTRNYNLNEQ AHDVTMALID GVIKQNVKLS HVYVDTVGPP ASYQKKLEQR FPGVKFTVAK
       190        200        210        220        230        240
KADSLYCMVS VASVVAKVTR DILVESLKRD PDEILGSGYP SDPKTVAWLK RNQTSLMGWP
       250        260        270        280        290        300
ANMVRFSWQT CQTLLDDASK NSIPIKWEEQ YMDSRKNAAQ KTKQLQLQMV AKPVRRKRLR

TLDNWYR     

Encoded by RNH201 gene


OTHER NAME(S):
RNH35


Nucleic acid sequence

        10         20         30         40         50         60
atggtacccc ccacggtaga agcatccttg gagtctcctt acactaagtc gtacttttca
        70         80         90        100        110        120
cctgtcccaa gcgcgctctt ggaacagaat gactctccaa taataatggg tatcgatgaa
       130        140        150        160        170        180
gctggcagag ggcccgtatt agggccaatg gtctacgcag tagcctattc tacacagaaa
       190        200        210        220        230        240
tatcaggacg aaactataat ccctaactac gagtttgacg actctaaaaa acttacagat
       250        260        270        280        290        300
cccatcagaa gaatgctgtt ttccaagata taccaggaca acgaagaact aacccagatc
       310        320        330        340        350        360
ggctacgcaa ctacgtgcat cactccgttg gatatctcta gaggaatgag caagttccca
       370        380        390        400        410        420
ccgacgagaa actataactt gaatgagcag gcgcacgatg tcacaatggc actcattgat
       430        440        450        460        470        480
ggcgtgataa agcagaacgt gaagctcagt cacgtgtatg tggatactgt tggaccacca
       490        500        510        520        530        540
gcgtcctatc agaagaaact ggagcaacga tttcctggtg tcaagtttac ggtcgccaag
       550        560        570        580        590        600
aaggcagact cgctctactg catggtcagt gtggcgagtg tcgttgcaaa agtgaccaga
       610        620        630        640        650        660
gatatactag tcgaatcctt gaagagggat cccgatgaga tcctgggttc tggatacccc
       670        680        690        700        710        720
tccgacccga agacagtcgc atggctgaag cgcaaccaga caagtcttat gggctggccc
       730        740        750        760        770        780
gccaacatgg taagattctc atggcagaca tgccagactc tgttagatga cgccagtaag
       790        800        810        820        830        840
aatagtatcc ctatcaagtg ggaagaacaa tatatggaca gcaggaagaa cgccgcacaa
       850        860        870        880        890        900
aaaaccaagc aattgcagct ccagatggtc gctaagcctg ttagaaggaa gaggctgaga
       910        920
accctagata attggtaccg gtga   

Last modification date: Oct. 2, 2011