Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor C subunit 3


DESCRIPTION:
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC3 supplies a catalytic site to the ATP site of RFC4.

MISCELLANEOUS:
Present with 3140 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the activator 1 small subunits family.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MSTSTEKRSK ENLPWVEKYR PETLDEVYGQ NEVITTVRKF VDEGKLPHLL FYGPPGTGKT
        70         80         90        100        110        120
STIVALAREI YGKNYSNMVL ELNASDDRGI DVVRNQIKDF ASTRQIFSKG FKLIILDEAD
       130        140        150        160        170        180
AMTNAAQNAL RRVIERYTKN TRFCVLANYA HKLTPALLSR CTRFRFQPLP QEAIERRIAN
       190        200        210        220        230        240
VLVHEKLKLS PNAEKALIEL SNGDMRRVLN VLQSCKATLD NPDEDEISDD VIYECCGAPR
       250        260        270        280        290        300
PSDLKAVLKS ILEDDWGTAH YTLNKVRSAK GLALIDLIEG IVKILEDYEL QNEETRVHLL
       310        320        330        340
TKLADIEYSI SKGGNDQIQG SAVIGAIKAS FENETVKANV  

Encoded by RFC3 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtcgacaa gtacagagaa gaggagcaaa gaaaaccttc catgggttga aaaatacaga
        70         80         90        100        110        120
cccgaaacgt tggacgaagt gtacggacaa aatgaggtga tcaccacagt tcgtaaattt
       130        140        150        160        170        180
gtagatgaag gtaaattgcc acatcttcta ttctatgggc ctccaggtac cggtaaaact
       190        200        210        220        230        240
tctacaattg ttgctttggc acgtgagata tatggtaaga attactcgaa catggttttg
       250        260        270        280        290        300
gagctgaatg catccgatga cagaggtatt gatgtggtga ggaatcaaat taaagacttt
       310        320        330        340        350        360
gcctctacaa gacaaatctt ctctaaaggg ttcaagttaa ttatactgga tgaagcagat
       370        380        390        400        410        420
gccatgacca atgccgcaca aaatgcgttg agaagagtta tagaacggta caccaagaat
       430        440        450        460        470        480
acgcggttct gtgtattggc caattatgcg cataaactta cacctgcgtt attgagtagg
       490        500        510        520        530        540
tgcacgagat tcagatttca gcccttgccc caagaggcca ttgagcgtcg gatagccaac
       550        560        570        580        590        600
gtcttggtgc atgagaagct gaagttgtct cctaatgcgg agaaggcctt aatagaactt
       610        620        630        640        650        660
tctaatgggg atatgagacg tgtgttaaac gttttgcagt cttgcaaagc tactttagac
       670        680        690        700        710        720
aatcccgacg aggatgagat cagtgacgac gttatctatg aatgctgtgg ggcacccaga
       730        740        750        760        770        780
ccaagtgact taaaggcagt attgaagtcg atactggagg acgattgggg taccgcccac
       790        800        810        820        830        840
tacacactta ataaggtacg cagtgccaag ggtctcgcgt tgatcgacct aatcgagggc
       850        860        870        880        890        900
atagtgaaga tactggaaga ctacgaactt caaaatgagg aaacaagagt gcatttgctt
       910        920        930        940        950        960
accaaactgg ccgatataga gtactcgata tccaagggtg gcaacgacca gattcagggc
       970        980        990       1000       1010       1020
agcgcggtca ttggcgccat caaggccagc ttcgagaacg aaactgttaa agccaacgta

taa     

Last modification date: Oct. 2, 2011