Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor C subunit 5


DESCRIPTION:
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.

MISCELLANEOUS:
Present with 5040 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the activator 1 small subunits family.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MSLWVDKYRP KSLNALSHNE ELTNFLKSLS DQPRDLPHLL LYGPNGTGKK TRCMALLESI
        70         80         90        100        110        120
FGPGVYRLKI DVRQFVTASN RKLELNVVSS PYHLEITPSD MGNNDRIVIQ ELLKEVAQME
       130        140        150        160        170        180
QVDFQDSKDG LAHRYKCVII NEANSLTKDA QAALRRTMEK YSKNIRLIMV CDSMSPIIAP
       190        200        210        220        230        240
IKSRCLLIRC PAPSDSEIST ILSDVVTNER IQLETKDILK RIAQASNGNL RVSLLMLESM
       250        260        270        280        290        300
ALNNELALKS SSPIIKPDWI IVIHKLTRKI VKERSVNSLI ECRAVLYDLL AHCIPANIIL
       310        320        330        340        350
KELTFSLLDV ETLNTTNKSS IIEYSSVFDE RLSLGNKAIF HLEGFIAKVM CCLD

Encoded by RFC5 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtcattgt gggtagataa atacagacct aagtccttga atgctctttc acataatgaa
        70         80         90        100        110        120
gagttgacaa attttctaaa atcgttatct gatcagcctc gtgatttacc tcatctttta
       130        140        150        160        170        180
ctgtatggac caaatggtac aggtaagaaa acgcgttgta tggcattatt ggagtccata
       190        200        210        220        230        240
tttggacctg gagtctatag attgaaaatt gatgtcagac aatttgtcac tgcttcgaac
       250        260        270        280        290        300
agaaaactag aactgaatgt ggtcagctcg ccataccatt tagagatcac gccaagtgat
       310        320        330        340        350        360
atgggtaaca atgatagaat tgtcatccaa gaactattga aagaagtggc tcaaatggaa
       370        380        390        400        410        420
caagtggact ttcaagattc taaggatgga cttgcccata gatataagtg tgttattatc
       430        440        450        460        470        480
aacgaggcga actcgttaac aaaagatgct caagctgctt taagacgtac catggaaaaa
       490        500        510        520        530        540
tactccaaaa acattaggtt gataatggtc tgcgattcga tgtcgcctat aattgctcct
       550        560        570        580        590        600
atcaaatccc gttgtctgtt gattcgttgt cctgcaccaa gcgatagcga aatttcaact
       610        620        630        640        650        660
atcttgtctg atgtggtgac aaatgaaaga atacaactag aaacaaagga tattttaaaa
       670        680        690        700        710        720
agaattgctc aggcatcgaa tggaaacttg cgagtctccc tattaatgct tgaatctatg
       730        740        750        760        770        780
gcactgaaca acgaattagc attgaaaagc agtagcccta taataaaacc agattggatt
       790        800        810        820        830        840
atagtgatcc ataaattaac gaggaaaatc gttaaagaga gatctgtcaa ttctttaatc
       850        860        870        880        890        900
gaatgcagag ctgtcctata cgatttacta gctcattgta tacctgccaa tatcatctta
       910        920        930        940        950        960
aaggaactaa cgttttcttt gttggatgtg gaaaccctga ataccacgaa taaatcgtcc
       970        980        990       1000       1010       1020
ataattgaat actcaagtgt ttttgacgaa agattatcac ttggaaacaa agcaatattc
      1030       1040       1050       1060
catttggaag ggttcatagc aaaagttatg tgctgtctag attaa 

Last modification date: Oct. 2, 2011