Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor C subunit 4


DESCRIPTION:
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair.

MISCELLANEOUS:
Present with 2760 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the activator 1 small subunits family.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MSKTLSLQLP WVEKYRPQVL SDIVGNKETI DRLQQIAKDG NMPHMIISGM PGIGKTTSVH
        70         80         90        100        110        120
CLAHELLGRS YADGVLELNA SDDRGIDVVR NQIKHFAQKK LHLPPGKHKI VILDEADSMT
       130        140        150        160        170        180
AGAQQALRRT MELYSNSTRF AFACNQSNKI IEPLQSRCAI LRYSKLSDED VLKRLLQIIK
       190        200        210        220        230        240
LEDVKYTNDG LEAIIFTAEG DMRQAINNLQ STVAGHGLVN ADNVFKIVDS PHPLIVKKML
       250        260        270        280        290        300
LASNLEDSIQ ILRTDLWKKG YSSIDIVTTS FRVTKNLAQV KESVRLEMIK EIGLTHMRIL
       310        320
EGVGTYLQLA SMLAKIHKLN NKA   

Encoded by RFC4 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtccaaaa ctttatcttt gcaacttcca tgggttgaga aataccgtcc ccaagtttta
        70         80         90        100        110        120
tctgatatag tcggtaataa agagaccatt gatagacttc agcaaatcgc taaagatggt
       130        140        150        160        170        180
aacatgcccc atatgatcat atcaggtatg ccaggtatag gtaagaccac ttcggtacat
       190        200        210        220        230        240
tgccttgctc acgagctcct tggccgctct tatgctgacg gtgttttaga gttgaacgct
       250        260        270        280        290        300
tcagatgaca gaggtattga tgtcgtcaga aaccaaataa aacattttgc ccagaagaaa
       310        320        330        340        350        360
ctacatttgc ctccagggaa acataaaatc gttattcttg atgaggcgga ttccatgact
       370        380        390        400        410        420
gctggtgctc agcaagcgtt gagaaggacc atggagctat attcgaactc tacaaggttt
       430        440        450        460        470        480
gcatttgctt gtaatcaatc aaacaagatc attgagccgc tgcaaagtag atgtgcgatt
       490        500        510        520        530        540
ttgaggtatt ctaaactatc cgatgaagac gttctaaaac gtcttttaca aatcataaag
       550        560        570        580        590        600
ctagaggatg ttaagtatac aaatgatggg ttagaagcaa tcatttttac agcggagggt
       610        620        630        640        650        660
gacatgagac aggccataaa caatctacaa agtacagtag caggacacgg tttagtgaac
       670        680        690        700        710        720
gcagacaatg tcttcaaaat tgttgattct cctcaccctc taatagtgaa gaaaatgtta
       730        740        750        760        770        780
ctagcctcca acctagaaga ttcaattcaa atcttaagaa cagatctttg gaaaaagggt
       790        800        810        820        830        840
tattcctcga ttgatatcgt cacaacatct ttccgcgtta ccaaaaactt agcacaagtg
       850        860        870        880        890        900
aaagaatcag taagattgga aatgataaaa gaaatcggtt tgacccatat gagaattctg
       910        920        930        940        950        960
gagggtgttg gaacgtactt gcaactggct agtatgttag cgaaaattca taaactaaat
       970
aataaagcct ga    

Last modification date: Oct. 2, 2011