Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor C subunit 2


DESCRIPTION:
Component of ATP-dependent clamp loader (RFC and RFC-like) complexes for DNA clamps, such as the POL30/PCNA homotrimer and the checkpoint clamp DDC1:MEC3:RAD17 complex. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Component of the replication factor C (RFC or activator 1) complex which loads POL30/PCNA and acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment. Component of the RFC-like complex CTF18-RFC which is required for efficient establishment of chromosome cohesion during S-phase and may load or unload POL30/PCNA. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. Component of the RFC-like ELG1-RFC complex which appears to have a role in DNA replication, replication fork re-start, recombination and repair. RFC2 binds ATP and single-stranded DNA.

MISCELLANEOUS:
Present with 4610 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the activator 1 small subunits family.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MFEGFGPNKK RKISKLAAEQ SLAQQPWVEK YRPKNLDEVT AQDHAVTVLK KTLKSANLPH
        70         80         90        100        110        120
MLFYGPPGTG KTSTILALTK ELYGPDLMKS RILELNASDE RGISIVREKV KNFARLTVSK
       130        140        150        160        170        180
PSKHDLENYP CPPYKIIILD EADSMTADAQ SALRRTMETY SGVTRFCLIC NYVTRIIDPL
       190        200        210        220        230        240
ASRCSKFRFK ALDASNAIDR LRFISEQENV KCDDGVLERI LDISAGDLRR GITLLQSASK
       250        260        270        280        290        300
GAQYLGDGKN ITSTQVEELA GVVPHDILIE IVEKVKSGDF DEIKKYVNTF MKSGWSAASV
       310        320        330        340        350
VNQLHEYYIT NDNFDTNFKN QISWLLFTTD SRLNNGTNEH IQLLNLLVKI SQL

Encoded by RFC2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtttgaag ggtttggtcc aaataaaaag cgtaagatat caaagttagc cgcagagcaa
        70         80         90        100        110        120
tcattggcac aacaaccctg ggttgagaaa tacaggccca aaaacctaga tgaagtgaca
       130        140        150        160        170        180
gctcaagatc atgccgttac tgttttgaag aaaaccttaa agtcagctaa tctaccacat
       190        200        210        220        230        240
atgttgtttt atggtccccc aggaactggt aaaacgtcta ccattttagc gctaacaaaa
       250        260        270        280        290        300
gaattatatg gacccgactt gatgaaatca agaatattgg agttgaacgc ttctgacgaa
       310        320        330        340        350        360
cgtggtatct ctattgtaag agaaaaggta aaaaattttg caagattgac tgtatccaag
       370        380        390        400        410        420
cctagcaaac atgacttgga gaactatccg tgcccaccgt ataagatcat tattctcgat
       430        440        450        460        470        480
gaggccgatt caatgactgc cgatgcacag agtgcattac gtagaacaat ggaaacctat
       490        500        510        520        530        540
tcaggagtaa caagattctg tttgatttgt aattatgtga caagaattat tgacccgcta
       550        560        570        580        590        600
gcgtcccgtt gttccaaatt tagattcaaa gccctagacg caagtaatgc cattgaccgt
       610        620        630        640        650        660
ttgaggttta tcagcgaaca agaaaatgtt aaatgtgatg atggagtttt ggaaaggatc
       670        680        690        700        710        720
ttggacattt cggcaggtga cttgagaaga ggcattacat tattacaatc cgcttccaag
       730        740        750        760        770        780
ggggcgcaat acttgggtga tggtaaaaac ataacttcta cccaagtgga agaattagct
       790        800        810        820        830        840
ggtgtggtac ctcatgacat tctaatagaa attgttgaaa aagtcaaaag tggtgatttt
       850        860        870        880        890        900
gatgagatta agaaatacgt caatacgttt atgaaaagcg gttggtctgc tgcgtcagta
       910        920        930        940        950        960
gttaaccaac tacatgaata ttacatcacc aacgataact ttgatacgaa cttcaaaaat
       970        980        990       1000       1010       1020
caaatatctt ggttgctgtt tactacggat tccagattaa acaacggaac gaacgagcac
      1030       1040       1050       1060
atccaattat taaacttatt ggttaaaata tcccaactct aa 

Last modification date: Oct. 2, 2011