Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor C subunit 1


DESCRIPTION:
Component of the ATP-dependent clamp loader RFC complex for the POL30/PCNA homotrimer DNA clamp. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double-stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. Replication factor C (RFC or activator 1) complex acts during elongation of primed DNA templates by DNA polymerase delta and epsilon. RFC has an essential but redundant activity in sister chromatid cohesion establishment.

MISCELLANEOUS:
Present with 2360 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MVNISDFFGK NKKSVRSSTS RPTRQVGSSK PEVIDLDTES DQESTNKTPK KMPVSNVIDV
        70         80         90        100        110        120
SETPEGEKKL PLPAKRKASS PTVKPASSKK TKPSSKSSDS ASNITAQDVL DKIPSLDLSN
       130        140        150        160        170        180
VHVKENAKFD FKSANSNADP DEIVSEIGSF PEGKPNCLLG LTIVFTGVLP TLERGASEAL
       190        200        210        220        230        240
AKRYGARVTK SISSKTSVVV LGDEAGPKKL EKIKQLKIKA IDEEGFKQLI AGMPAEGGDG
       250        260        270        280        290        300
EAAEKARRKL EEQHNIATKE AELLVKKEEE RSKKLAATRV SGGHLERDNV VREEDKLWTV
       310        320        330        340        350        360
KYAPTNLQQV CGNKGSVMKL KNWLANWENS KKNSFKHAGK DGSGVFRAAM LYGPPGIGKT
       370        380        390        400        410        420
TAAHLVAQEL GYDILEQNAS DVRSKTLLNA GVKNALDNMS VVGYFKHNEE AQNLNGKHFV
       430        440        450        460        470        480
IIMDEVDGMS GGDRGGVGQL AQFCRKTSTP LILICNERNL PKMRPFDRVC LDIQFRRPDA
       490        500        510        520        530        540
NSIKSRLMTI AIREKFKLDP NVIDRLIQTT RGDIRQVINL LSTISTTTKT INHENINEIS
       550        560        570        580        590        600
KAWEKNIALK PFDIAHKMLD GQIYSDIGSR NFTLNDKIAL YFDDFDFTPL MIQENYLSTR
       610        620        630        640        650        660
PSVLKPGQSH LEAVAEAANC ISLGDIVEKK IRSSEQLWSL LPLHAVLSSV YPASKVAGHM
       670        680        690        700        710        720
AGRINFTAWL GQNSKSAKYY RLLQEIHYHT RLGTSTDKIG LRLDYLPTFR KRLLDPFLKQ
       730        740        750        760        770        780
GADAISSVIE VMDDYYLTKE DWDSIMEFFV GPDVTTAIIK KIPATVKSGF TRKYNSMTHP
       790        800        810        820        830        840
VAIYRTGSTI GGGGVGTSTS TPDFEDVVDA DDNPVPADDE ETQDSSTDLK KDKLIKQKAK
       850        860
PTKRKTATSK PGGSKKRKTK A   

Encoded by RFC1 gene


OTHER NAME(S):
CDC44


Nucleic acid sequence

        10         20         30         40         50         60
atggtcaata tttctgattt ctttggtaaa aataagaaat ccgtaagatc gtcaacatcc
        70         80         90        100        110        120
agacctacga gacaggtggg ttcgtctaaa ccagaagtta tcgacttaga tactgaatct
       130        140        150        160        170        180
gaccaagaat caaccaataa aacaccaaag aagatgcctg taagtaatgt aattgatgta
       190        200        210        220        230        240
tcagagacac ctgaaggaga aaaaaagcta cctcttcccg ctaagaggaa ggcctcttca
       250        260        270        280        290        300
ccaactgtaa agccagcaag ttcaaaaaaa accaaaccct cctctaaaag ttcagactcg
       310        320        330        340        350        360
gcttcgaata ttacggctca agatgtacta gataagattc catccttgga tctgtcaaat
       370        380        390        400        410        420
gttcatgtga aagagaatgc taaatttgat ttcaaaagcg caaattcaaa tgcggatcca
       430        440        450        460        470        480
gatgaaattg tttctgaaat aggtagtttt ccagaaggaa agccaaattg tttattaggt
       490        500        510        520        530        540
ctaacaattg tcttcacagg tgttctgcca accttggagc gtggtgcatc ggaagcttta
       550        560        570        580        590        600
gcgaaaagat acggagcaag ggttactaaa tcaatttcaa gcaaaacttc tgtagtagtc
       610        620        630        640        650        660
ttaggtgatg aagcagggcc aaagaaacta gaaaaaatca agcaattgaa aatcaaagcc
       670        680        690        700        710        720
atagacgaag aaggctttaa gcaattaatt gctgggatgc ctgctgaagg tggtgacggg
       730        740        750        760        770        780
gaagctgccg aaaaagcacg tcgaaaatta gaagaacagc ataatattgc taccaaagaa
       790        800        810        820        830        840
gcagaattgc ttgttaagaa agaagaggaa aggtcgaaga agcttgcagc cactagagtt
       850        860        870        880        890        900
tctggtggcc atctggagag agacaatgtg gttagggaag aagataaatt gtggacagta
       910        920        930        940        950        960
aagtacgcac caacgaatct acaacaggtc tgtggtaaca aaggaagtgt aatgaaatta
       970        980        990       1000       1010       1020
aaaaactggt tggctaattg ggaaaattca aagaaaaata gttttaaaca tgctggtaaa
      1030       1040       1050       1060       1070       1080
gatggctctg gtgttttcag ggctgcaatg ttatacggtc ctcctggtat tgggaagaca
      1090       1100       1110       1120       1130       1140
actgctgctc atttagttgc acaagagctt gggtatgaca ttcttgaaca aaacgcttct
      1150       1160       1170       1180       1190       1200
gatgttcgct ctaaaacttt acttaatgcc ggtgttaaaa acgctcttga taacatgtct
      1210       1220       1230       1240       1250       1260
gtagtgggat atttcaaaca caacgaagaa gctcaaaatt tgaacgggaa acattttgtc
      1270       1280       1290       1300       1310       1320
attatcatgg atgaagttga tggtatgagc ggaggcgata gggggggagt tggccagctg
      1330       1340       1350       1360       1370       1380
gcacagtttt gtcgtaagac gtcaactcca ttgattctaa tttgtaatga acgtaattta
      1390       1400       1410       1420       1430       1440
ccaaaaatga gaccattcga tagagtatgt cttgatatcc aatttagaag acctgatgct
      1450       1460       1470       1480       1490       1500
aacagtatca aatcaagatt aatgacaatt gcgatcagag aaaagttcaa acttgatcca
      1510       1520       1530       1540       1550       1560
aatgtcattg ataggttgat acagactaca agaggtgata tccgccaggt tattaatcta
      1570       1580       1590       1600       1610       1620
ctttcaacga tatctacgac tactaaaacc ataaaccatg aaaatatcaa cgagatctca
      1630       1640       1650       1660       1670       1680
aaggcatggg aaaagaatat tgccctaaaa ccctttgaca ttgcccataa aatgttggat
      1690       1700       1710       1720       1730       1740
ggacaaatat actcagacat aggttcaagg aactttacac tgaatgataa gatcgcatta
      1750       1760       1770       1780       1790       1800
tattttgatg actttgattt cacaccccta atgattcagg aaaactattt atctacaaga
      1810       1820       1830       1840       1850       1860
ccaagcgttt tgaaacccgg ccagtcacat ttggaagcag ttgctgaggc ggctaactgt
      1870       1880       1890       1900       1910       1920
atttcattag gtgatattgt cgaaaagaag attcgtagta gtgagcaatt atggagtctt
      1930       1940       1950       1960       1970       1980
ttgcctttgc atgctgttct ttcatctgtt tatccagcat caaaagttgc cggccatatg
      1990       2000       2010       2020       2030       2040
gcgggaagaa tcaatttcac agcttggttg ggccaaaatt ctaaatctgc caaatattat
      2050       2060       2070       2080       2090       2100
aggttactgc aagagataca ttatcataca agactaggta catctactga taaaattgga
      2110       2120       2130       2140       2150       2160
ctaaggctgg attacctacc aacttttaga aaaagattat tggacccatt tttgaagcaa
      2170       2180       2190       2200       2210       2220
ggtgctgatg caatttcatc tgtcatagag gtaatggacg attactattt gaccaaagaa
      2230       2240       2250       2260       2270       2280
gactgggatt ctattatgga gtttttcgta ggtcctgatg tgaccacagc cattatcaaa
      2290       2300       2310       2320       2330       2340
aagataccag ctacggttaa aagtggattc acgcggaaat acaacagtat gacacatcca
      2350       2360       2370       2380       2390       2400
gtcgcaattt acagaacagg tagtactatt ggtggtggtg gtgttggcac cagtactagc
      2410       2420       2430       2440       2450       2460
acccccgatt tcgaagatgt cgttgacgca gatgacaatc cagttcccgc agatgatgaa
      2470       2480       2490       2500       2510       2520
gaaacacaag atagtagcac agacttgaag aaggataaac ttatcaagca gaaagccaaa
      2530       2540       2550       2560       2570       2580
cctacgaaga ggaagactgc caccagtaaa cctggtggta gcaaaaaaag gaaaacgaaa

gcatga     

Last modification date: Oct. 2, 2011