Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Proliferating cell nuclear antigen


DESCRIPTION:
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Involved in DNA repair.

STRUCTURE SIMILARITY:
Belongs to the PCNA family.


POST-TRANSLATIONAL MODIFICATION:
Monoubiquitinated on Lys-164 by the UBC2/RAD18 complex upon DNA damage, and then polyubiquitinated through 'Lys-63'-linkage by UBC13/MMS2. Ubiquitination is required for UBC2-mediated DNA repair.
Sumoylated on Lys-164, and to a lesser extent on Lys-127 by the UBC9/SIZ1 complex during S-phase; which impairs ubiquitination and function in DNA repair.


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MLEAKFEEAS LFKRIIDGFK DCVQLVNFQC KEDGIIAQAV DDSRVLLVSL EIGVEAFQEY
        70         80         90        100        110        120
RCDHPVTLGM DLTSLSKILR CGNNTDTLTL IADNTPDSII LLFEDTKKDR IAEYSLKLMD
       130        140        150        160        170        180
IDADFLKIEE LQYDSTLSLP SSEFSKIVRD LSQLSDSINI MITKETIKFV ADGDIGSGSV
       190        200        210        220        230        240
IIKPFVDMEH PETSIKLEMD QPVDLTFGAK YLLDIIKGSS LSDRVGIRLS SEAPALFQFD
       250
LKSGFLQFFL APKFNDEE    

Encoded by POL30 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgttagaag caaaatttga agaagcatcc cttttcaaga gaataattga tggtttcaaa
        70         80         90        100        110        120
gattgtgtcc agttggtcaa tttccaatgt aaagaagatg gtatcattgc acaagctgtc
       130        140        150        160        170        180
gatgactcaa gagttctatt ggtctccttg gaaataggtg tcgaagcctt ccaagaatat
       190        200        210        220        230        240
agatgtgacc atcctgttac gttaggtatg gatctaacct cactaagtaa aatcctacgt
       250        260        270        280        290        300
tgtggtaaca acaccgatac attaacacta attgctgaca acacaccgga ttccatcatc
       310        320        330        340        350        360
ttattatttg aggataccaa gaaagaccgt atagccgaat actctctgaa attgatggat
       370        380        390        400        410        420
atcgatgctg atttcttaaa gattgaagaa ttacagtacg actccaccct gtcattgcca
       430        440        450        460        470        480
tcttccgaat tctctaaaat tgttcgtgac ttgtcccaat tgagtgattc tattaatatc
       490        500        510        520        530        540
atgatcacca aagaaacaat aaagtttgta gctgacggtg atatcggatc aggttcagtc
       550        560        570        580        590        600
ataataaaac cattcgtgga tatggaacat cctgaaacaa gcatcaaact tgaaatggat
       610        620        630        640        650        660
caacctgtcg acttgacgtt cggagctaaa tatttattgg acatcattaa gggctcctcc
       670        680        690        700        710        720
ctttctgata gagttggtat caggctctcc agcgaagctc ctgctttatt ccaatttgat
       730        740        750        760        770
ttgaagagtg ggttcctaca gtttttcttg gctcctaaat ttaatgacga agaataa

Last modification date: Oct. 2, 2011