Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor A protein 2


DESCRIPTION:
Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

MISCELLANEOUS:
Present with 6080 molecules/cell in log phase SD medium.


POST-TRANSLATIONAL MODIFICATION:
The N-terminus is blocked.
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis).


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MATYQPYNEY SSVTGGGFEN SESRPGSGES ETNTRVNTLT PVTIKQILES KQDIQDGPFV
        70         80         90        100        110        120
SHNQELHHVC FVGVVRNITD HTANIFLTIE DGTGQIEVRK WSEDANDLAA GNDDSSGKGY
       130        140        150        160        170        180
GSQVAQQFEI GGYVKVFGAL KEFGGKKNIQ YAVIKPIDSF NEVLTHHLEV IKCHSIASGM
       190        200        210        220        230        240
MKQPLESASN NNGQSLFVKD DNDTSSGSSP LQRILEFCKK QCEGKDANSF AVPIPLISQS
       250        260        270
LNLDETTVRN CCTTLTDQGF IYPTFDDNNF FAL  

Encoded by RFA2 gene


OTHER NAME(S):
BUF1
RPA2


Nucleic acid sequence

        10         20         30         40         50         60
atggcaacct atcaaccata taacgaatat tcatcagtaa cgggcggtgg ctttgagaac
        70         80         90        100        110        120
tctgagtccc gcccaggtag tggggagtcg gaaactaaca ctagagttaa caccttgaca
       130        140        150        160        170        180
cctgtgacga tcaaacaaat tctagagtcc aaacaggata ttcaggacgg ccccttcgtt
       190        200        210        220        230        240
tcgcataacc aagaacttca tcacgtttgt tttgtaggtg tggtgagaaa cattacagac
       250        260        270        280        290        300
catactgcaa atattttttt aactattgag gatggaactg gtcaaataga agtgagaaaa
       310        320        330        340        350        360
tggagcgaag atgcaaatga cttggctgcc ggtaacgatg actcttctgg taaaggttat
       370        380        390        400        410        420
ggttcgcaag tcgcccaaca atttgaaatt ggcggttacg taaaagtttt tggtgctttg
       430        440        450        460        470        480
aaagagtttg gtggtaagaa aaatatacag tatgcggtga ttaagcccat agattcattc
       490        500        510        520        530        540
aatgaagtgt tgacgcatca cttggaagtc atcaaatgtc attccatagc cagtggaatg
       550        560        570        580        590        600
atgaaacaac ctttggagag tgcatccaac aacaatgggc aatcattatt tgtcaaggat
       610        620        630        640        650        660
gataacgata catcttccgg ctccagtccg ttacaaagaa ttctagaatt ttgtaagaag
       670        680        690        700        710        720
caatgtgagg gcaaagacgc taattcattc gctgttccca ttccattgat ctcgcaatcc
       730        740        750        760        770        780
ttgaatttgg atgaaactac cgtcagaaac tgctgtacga ccttgactga ccagggtttt
       790        800        810        820
atctacccaa cttttgatga caataacttc tttgccctat ga 

Last modification date: Oct. 2, 2011