Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Replication factor A protein 1


DESCRIPTION:
Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.

MISCELLANEOUS:
Present with 4100 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the replication factor A protein 1 family.


POST-TRANSLATIONAL MODIFICATION:
The N-terminus is blocked.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MSSVQLSRGD FHSIFTNKQR YDNPTGGVYQ VYNTRKSDGA NSNRKNLIMI SDGIYHMKAL
        70         80         90        100        110        120
LRNQAASKFQ SMELQRGDII RVIIAEPAIV RERKKYVLLV DDFELVQSRA DMVNQTSTFL
       130        140        150        160        170        180
DNYFSEHPNE TLKDEDITDS GNVANQTNAS NAGVPDMLHS NSNLNANERK FANENPNSQK
       190        200        210        220        230        240
TRPIFAIEQL SPYQNVWTIK ARVSYKGEIK TWHNQRGDGK LFNVNFLDTS GEIRATAFND
       250        260        270        280        290        300
FATKFNEILQ EGKVYYVSKA KLQPAKPQFT NLTHPYELNL DRDTVIEECF DESNVPKTHF
       310        320        330        340        350        360
NFIKLDAIQN QEVNSNVDVL GIIQTINPHF ELTSRAGKKF DRRDITIVDD SGFSISVGLW
       370        380        390        400        410        420
NQQALDFNLP EGSVAAIKGV RVTDFGGKSL SMGFSSTLIP NPEIPEAYAL KGWYDSKGRN
       430        440        450        460        470        480
ANFITLKQEP GMGGQSAASL TKFIAQRITI ARAQAENLGR SEKGDFFSVK AAISFLKVDN
       490        500        510        520        530        540
FAYPACSNEN CNKKVLEQPD GTWRCEKCDT NNARPNWRYI LTISIIDETN QLWLTLFDDQ
       550        560        570        580        590        600
AKQLLGVDAN TLMSLKEEDP NEFTKITQSI QMNEYDFRIR AREDTYNDQS RIRYTVANLH
       610        620
SLNYRAEADY LADELSKALL A   

Encoded by RFA1 gene


OTHER NAME(S):
BUF2
FUN3
RPA1


Nucleic acid sequence

        10         20         30         40         50         60
atgagcagtg ttcaactttc gaggggcgat tttcatagca tcttcaccaa taagcaaagg
        70         80         90        100        110        120
tacgataatc ccaccggtgg cgtttatcaa gtttataaca ccaggaaatc tgatggggct
       130        140        150        160        170        180
aacagcaaca gaaagaattt gatcatgatt tccgatggta tttaccatat gaaggctctg
       190        200        210        220        230        240
ttgagaaacc aagctgcatc caagttccag tcaatggaac tacaaagggg tgatatcatt
       250        260        270        280        290        300
cgcgtgataa ttgcagaacc tgctattgtc agggaaagaa agaaatacgt tcttttagta
       310        320        330        340        350        360
gatgactttg agttggtcca gtcgcgtgct gatatggtca accaaactag tacttttttg
       370        380        390        400        410        420
gataactatt tctcagagca tccaaatgaa accttaaaag acgaagatat aactgacagt
       430        440        450        460        470        480
ggtaatgttg ccaatcaaac aaacgccagc aatgctggtg tccctgatat gctgcattca
       490        500        510        520        530        540
aactcaaact tgaatgcaaa tgagagaaaa ttcgccaatg aaaaccctaa ttcgcaaaaa
       550        560        570        580        590        600
accagaccaa tttttgccat cgaacaactg tctccatacc aaaacgtttg gactatcaaa
       610        620        630        640        650        660
gcaagagttt cctacaaggg agaaattaaa acgtggcaca atcaaagagg tgatggtaaa
       670        680        690        700        710        720
ctattcaatg tcaacttctt ggatacctct ggagaaatcc gagccacggc gtttaatgat
       730        740        750        760        770        780
tttgctacaa aatttaacga aattttacaa gaaggcaaag tatactatgt atcaaaggca
       790        800        810        820        830        840
aaactccaac cagctaagcc ccaatttact aatctaacac acccttatga actgaatttg
       850        860        870        880        890        900
gatagagaca ctgttataga agaatgtttc gatgaaagta atgttccgaa aacccatttc
       910        920        930        940        950        960
aatttcatca aactagatgc tattcagaac caggaagtaa attccaacgt agacgtcctc
       970        980        990       1000       1010       1020
ggtattatcc aaactataaa cccacatttt gagctaactt caagggctgg gaagaaattc
      1030       1040       1050       1060       1070       1080
gatcgtcgtg acatcacaat tgttgacgac tctgggtttt ctatctctgt tggcctatgg
      1090       1100       1110       1120       1130       1140
aatcagcaag cccttgattt caaccttcct gaaggttctg ttgctgccat taaaggtgtt
      1150       1160       1170       1180       1190       1200
cgtgtgacgg attttggtgg caaatctttg tctatgggat tttctagtac cctgattccg
      1210       1220       1230       1240       1250       1260
aatccagaaa ttcctgaggc atatgcctta aagggttggt atgattccaa gggccgcaac
      1270       1280       1290       1300       1310       1320
gcaaacttca tcactttaaa gcaagaaccc ggtatgggtg gtcaatcggc tgctagctta
      1330       1340       1350       1360       1370       1380
acaaaattca ttgctcagcg tattactatt gctagagctc aagctgaaaa tctaggaaga
      1390       1400       1410       1420       1430       1440
agcgagaaag gtgacttttt tagtgttaaa gctgctataa gtttcttaaa agttgataat
      1450       1460       1470       1480       1490       1500
tttgcatatc ctgcctgttc taatgagaat tgtaataaga aagttctgga acagcctgat
      1510       1520       1530       1540       1550       1560
ggtacttgga gatgtgagaa gtgcgacacc aataatgcaa ggccaaattg gagatacatc
      1570       1580       1590       1600       1610       1620
ttgacaatat caattattga cgaaaccaat caactatggc tcactttatt tgacgaccaa
      1630       1640       1650       1660       1670       1680
gctaaacaat tattgggtgt tgatgctaat acattaatgt ctttgaagga agaagacccc
      1690       1700       1710       1720       1730       1740
aacgaattca caaaaattac tcaaagtatc caaatgaacg aatatgactt taggattaga
      1750       1760       1770       1780       1790       1800
gcgcgtgagg atacatacaa tgatcaaagc agaattagat ataccgttgc taacctacac
      1810       1820       1830       1840       1850       1860
agcttgaatt acagggctga agccgactat cttgccgatg agttatccaa ggctttgtta

gcttaa     

Last modification date: Oct. 2, 2011