Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA replication licensing factor MCM7


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.
Present with 166 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSAALPSIQL PVDYNNLFNE ITDFLVTFKQ DTLSSDATRN ENEDENLDAE NIEQHLLEKG
        70         80         90        100        110        120
PKYMAMLQKV ANRELNSVII DLDDILQYQN EKFLQGTQAD DLVSAIQQNA NHFTELFCRA
       130        140        150        160        170        180
IDNNMPLPTK EIDYKDDVLD VILNQRRLRN ERMLSDRTNE IRSENLMDTT MDPPSSMNDA
       190        200        210        220        230        240
LREVVEDETE LFPPNLTRRY FLYFKPLSQN CARRYRKKAI SSKPLSVRQI KGDFLGQLIT
       250        260        270        280        290        300
VRGIITRVSD VKPAVEVIAY TCDQCGYEVF QEVNSRTFTP LSECTSEECS QNQTKGQLFM
       310        320        330        340        350        360
STRASKFSAF QECKIQELSQ QVPVGHIPRS LNIHVNGTLV RSLSPGDIVD VTGIFLPAPY
       370        380        390        400        410        420
TGFKALKAGL LTETYLEAQF VRQHKKKFAS FSLTSDVEER VMELITSGDV YNRLAKSIAP
       430        440        450        460        470        480
EIYGNLDVKK ALLLLLVGGV DKRVGDGMKI RGDINVCLMG DPGVAKSQLL KAICKISPRG
       490        500        510        520        530        540
VYTTGKGSSG VGLTAAVMKD PVTDEMILEG GALVLADNGI CCIDEFDKMD ESDRTAIHEV
       550        560        570        580        590        600
MEQQTISISK AVINTNPGAR TSILAAANPL YGRINPRLSP LDNINLPAAL LSRFDILFLM
       610        620        630        640        650        660
LDIPSRDDDE KLAEHVTYVH MHNKQPDLDF TPVEPSKMRE YIAYAKTKRP VMSEAVNDYV
       670        680        690        700        710        720
VQAYIRLRQD SKREMDSKFS FGQATPRTLL GIIRLSQALA KLRLADMVDI DDVEEALRLV
       730        740        750        760        770        780
RVSKESLYQE TNKSKEDESP TTKIFTIIKK MLQETGKNTL SYENIVKTVR LRGFTMLQLS
       790        800        810        820        830        840
NCIQEYSYLN VWHLINEGNT LKFVDDGTMD TDQEDSLVST PKLAPQTTAS ANVSAQDSDI

DLQDA     

Encoded by MCM7 gene


OTHER NAME(S):
CDC47


Nucleic acid sequence

        10         20         30         40         50         60
atgagtgcgg cacttccatc aattcagctt cctgtcgact ataataatct ttttaatgaa
        70         80         90        100        110        120
atcaccgatt ttttggtgac tttcaagcag gatacgttgt cttctgatgc aacacgaaat
       130        140        150        160        170        180
gagaacgaag atgaaaattt ggatgctgag aatatcgaac aacatctatt ggaaaagggg
       190        200        210        220        230        240
cccaagtaca tggcgatgct acagaaagtg gctaataggg aactgaattc cgtgattatt
       250        260        270        280        290        300
gatttagatg acattctaca gtatcaaaac gaaaagtttc tccagggcac acaggcagac
       310        320        330        340        350        360
gaccttgtgt ctgccattca acaaaacgct aatcacttca ctgaattatt ttgccgtgcc
       370        380        390        400        410        420
attgacaaca acatgccgct accaacaaaa gaaattgact acaaggacga cgttcttgat
       430        440        450        460        470        480
gttattttaa accaaaggag actgaggaat gagagaatgc tctcggacag gaccaatgag
       490        500        510        520        530        540
atccgaagcg aaaacctcat ggataccacg atggatccac cctcttccat gaatgatgcg
       550        560        570        580        590        600
ttaagagaag ttgtcgagga tgaaactgag ttatttcctc ctaatttaac tagacgctat
       610        620        630        640        650        660
ttcctttatt ttaagcctct atcgcaaaat tgtgctcgtc gttacaggaa gaaagcaatt
       670        680        690        700        710        720
agttctaaac cattatctgt taggcagatt aaaggtgact tcctaggcca attgattacc
       730        740        750        760        770        780
gtcagaggta ttatcaccag agtttctgat gtcaaaccag ctgtggaagt tatcgcatat
       790        800        810        820        830        840
acctgcgatc aatgtgggta cgaagttttc caagaggtca actctcgtac ttttactccg
       850        860        870        880        890        900
ttgtcagaat gtacttccga agaatgttcc caaaatcaaa caaagggtca attgtttatg
       910        920        930        940        950        960
agtacacgag cttccaaatt tagtgccttt caggaatgta aaattcagga gttatcacaa
       970        980        990       1000       1010       1020
caagtgccag tgggtcatat tcctaggtcg ctaaatatcc acgttaacgg gacgctggta
      1030       1040       1050       1060       1070       1080
agatctttat cgcctggtga tatcgtcgat gtaactggta tattcttgcc agcgccctac
      1090       1100       1110       1120       1130       1140
accggtttca aagctttgaa agctggctta ctgacagaaa cttatttaga ggcacagttt
      1150       1160       1170       1180       1190       1200
gtccgtcaac acaagaagaa atttgcgtct ttcagtctga cttctgatgt agaagagaga
      1210       1220       1230       1240       1250       1260
gttatggaat taatcacctc tggtgatgtt tataataggc tggcaaaatc tattgcaccg
      1270       1280       1290       1300       1310       1320
gaaatctacg gtaacttgga cgtaaagaaa gcgttgctgc tattacttgt cggaggtgtt
      1330       1340       1350       1360       1370       1380
gataaaaggg taggtgacgg tatgaaaatc agaggtgata tcaatgtttg tctgatgggt
      1390       1400       1410       1420       1430       1440
gatcccggtg ttgccaaatc tcaactgctg aaggccattt gcaaaatatc acctcgagga
      1450       1460       1470       1480       1490       1500
gtgtatacca ctggtaaggg ttcctcaggc gttggtctga ccgctgccgt catgaaagat
      1510       1520       1530       1540       1550       1560
cctgtcacgg atgaaatgat tctagaaggt ggtgccttag tactcgctga taacggtatt
      1570       1580       1590       1600       1610       1620
tgttgtatcg atgaatttga taagatggat gaaagtgaca gaacggcaat ccatgaagtt
      1630       1640       1650       1660       1670       1680
atggaacaac aaacaatttc gatatccaag gcggttatca atacaaatcc gggcgccaga
      1690       1700       1710       1720       1730       1740
acctcaatct tagcggcagc aaatccgttg tatggtagaa ttaatcctag attatcacct
      1750       1760       1770       1780       1790       1800
ctggacaata taaatctacc agctgcttta ctatccagat ttgatattct cttcttaatg
      1810       1820       1830       1840       1850       1860
ttagatatac caagtagaga tgacgacgaa aaattagccg agcacgttac atatgtgcat
      1870       1880       1890       1900       1910       1920
atgcataata aacaaccgga tttggacttc actccagtag aaccctctaa aatgagagag
      1930       1940       1950       1960       1970       1980
tacattgcct atgctaagac aaagaggcca gtgatgagtg aagctgtaaa cgattatgtg
      1990       2000       2010       2020       2030       2040
gtgcaagctt acatcagatt aagacaagac tccaagagag aaatggattc caaattttcc
      2050       2060       2070       2080       2090       2100
tttggtcaag ccactccgag aactttacta ggtattataa gattatctca agcgctagca
      2110       2120       2130       2140       2150       2160
aagttaaggt tggctgatat ggtggatata gatgatgtgg aggaagcctt gaggttggtc
      2170       2180       2190       2200       2210       2220
cgagtctcca aggaatcatt gtatcaagaa accaacaaat ctaaagaaga tgaaagcccc
      2230       2240       2250       2260       2270       2280
acaacgaaaa tctttacgat catcaagaaa atgttacaag aaactggtaa aaacacgcta
      2290       2300       2310       2320       2330       2340
tcatatgaaa atattgtgaa aactgtaaga ctaagagggt ttaccatgct acaattaagc
      2350       2360       2370       2380       2390       2400
aactgtatcc aagaatattc ttacttaaac gtctggcatt taattaatga gggtaacact
      2410       2420       2430       2440       2450       2460
ttgaaattcg tggatgacgg taccatggac acagaccagg aagactcact agtgagcacg
      2470       2480       2490       2500       2510       2520
cctaaactcg caccacaaac gaccgcttcc gctaatgtga gcgcccaaga ttctgatatc
      2530
gatctacaag acgcttga    

Last modification date: Oct. 2, 2011