Mus musculus L. (mouse) [MMU]

FULL NAME: DNA (cytosine-5)-methyltransferase 3A


DESCRIPTION:
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

STRUCTURE SIMILARITY:
Contains 1 GATA-type zinc finger.
Belongs to the C5-methyltransferase family.
Contains 1 PWWP domain.
Contains 1 PHD-type zinc finger.
Contains 1 ADD domain.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.


ENZYME REGULATION:
Activated by binding to the regulatory factor DNMT3L.


POST-TRANSLATIONAL MODIFICATION:
Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.


PROTEIN TYPE(S):
DNA methyltransferase


RELATED PATHWAY(S):
DNA replication
heterochromatin formation


Amino acids sequence

        10         20         30         40         50         60
MPSSGPGDTS SSSLEREDDR KEGEEQEENR GKEERQEPSA TARKVGRPGR KRKHPPVESS
        70         80         90        100        110        120
DTPKDPAVTT KSQPMAQDSG PSDLLPNGDL EKRSEPQPEE GSPAAGQKGG APAEGEGTET
       130        140        150        160        170        180
PPEASRAVEN GCCVTKEGRG ASAGEGKEQK QTNIESMKME GSRGRLRGGL GWESSLRQRP
       190        200        210        220        230        240
MPRLTFQAGD PYYISKRKRD EWLARWKREA EKKAKVIAVM NAVEENQASG ESQKVEEASP
       250        260        270        280        290        300
PAVQQPTDPA SPTVATTPEP VGGDAGDKNA TKAADDEPEY EDGRGFGIGE LVWGKLRGFS
       310        320        330        340        350        360
WWPGRIVSWW MTGRSRAAEG TRWVMWFGDG KFSVVCVEKL MPLSSFCSAF HQATYNKQPM
       370        380        390        400        410        420
YRKAIYEVLQ VASSRAGKLF PACHDSDESD SGKAVEVQNK QMIEWALGGF QPSGPKGLEP
       430        440        450        460        470        480
PEEEKNPYKE VYTDMWVEPE AAAYAPPPPA KKPRKSTTEK PKVKEIIDER TRERLVYEVR
       490        500        510        520        530        540
QKCRNIEDIC ISCGSLNVTL EHPLFIGGMC QNCKNCFLEC AYQYDDDGYQ SYCTICCGGR
       550        560        570        580        590        600
EVLMCGNNNC CRCFCVECVD LLVGPGAAQA AIKEDPWNCY MCGHKGTYGL LRRREDWPSR
       610        620        630        640        650        660
LQMFFANNHD QEFDPPKVYP PVPAEKRKPI RVLSLFDGIA TGLLVLKDLG IQVDRYIASE
       670        680        690        700        710        720
VCEDSITVGM VRHQGKIMYV GDVRSVTQKH IQEWGPFDLV IGGSPCNDLS IVNPARKGLY
       730        740        750        760        770        780
EGTGRLFFEF YRLLHDARPK EGDDRPFFWL FENVVAMGVS DKRDISRFLE SNPVMIDAKE
       790        800        810        820        830        840
VSAAHRARYF WGNLPGMNRP LASTVNDKLE LQECLEHGRI AKFSKVRTIT TRSNSIKQGK
       850        860        870        880        890        900
DQHFPVFMNE KEDILWCTEM ERVFGFPVHY TDVSNMSRLA RQRLLGRSWS VPVIRHLFAP

LKEYFACV     

Encoded by Dnmt3a gene

FULL NAME: DNA methyltransferase 3A


OTHER NAME(S):
AU015806
W91664


Nucleic acid sequence

        10         20         30         40         50         60
atgccctcca gcggccccgg ggacaccagc agctcctctc tggagcggga ggatgatcga
        70         80         90        100        110        120
aaggaaggag aggaacagga ggagaaccgt ggcaaggaag agcgccagga gcccagcgcc
       130        140        150        160        170        180
acggcccgga aggtggggag gcctggccgg aagcgcaagc acccaccggt ggaaagcagt
       190        200        210        220        230        240
gacaccccca aggacccagc agtgaccacc aagtctcagc ccatggccca ggactctggc
       250        260        270        280        290        300
ccctcagatc tgctacccaa tggagacttg gagaagcgga gtgaacccca acctgaggag
       310        320        330        340        350        360
gggagcccag ctgcagggca gaagggtggg gccccagctg aaggagaggg aactgagacc
       370        380        390        400        410        420
ccaccagaag cctccagagc tgtggagaat ggctgctgtg tgaccaagga aggccgtgga
       430        440        450        460        470        480
gcctctgcag gagagggcaa agaacagaag cagaccaaca tcgaatccat gaaaatggag
       490        500        510        520        530        540
ggctcccggg gccgactgcg aggtggcttg ggctgggagt ccagcctccg tcagcgaccc
       550        560        570        580        590        600
atgccaagac tcaccttcca ggcaggggac ccctactaca tcagcaaacg gaaacgggat
       610        620        630        640        650        660
gagtggctgg cacgttggaa aagggaggct gagaagaaag ccaaggtaat tgcagtaatg
       670        680        690        700        710        720
aatgctgtgg aagagaacca ggcctctgga gagtctcaga aggtggagga ggccagccct
       730        740        750        760        770        780
cctgctgtgc agcagcccac ggaccctgct tctccgactg tggccaccac ccctgagcca
       790        800        810        820        830        840
gtaggagggg atgctgggga caagaatgct accaaagcag ccgacgatga gcctgagtat
       850        860        870        880        890        900
gaggatggcc ggggctttgg cattggagag ctggtgtggg ggaaacttcg gggcttctcc
       910        920        930        940        950        960
tggtggccag gccgaattgt gtcttggtgg atgacaggcc ggagccgagc agctgaaggc
       970        980        990       1000       1010       1020
actcgctggg tcatgtggtt cggagatggc aagttctcag tggtgtgtgt ggagaagctc
      1030       1040       1050       1060       1070       1080
atgccgctga gctccttctg cagtgcattc caccaggcca cctacaacaa gcagcccatg
      1090       1100       1110       1120       1130       1140
taccgcaaag ccatctacga agtcctccag gtggccagca gccgtgccgg gaagctgttt
      1150       1160       1170       1180       1190       1200
ccagcttgcc atgacagtga tgaaagtgac agtggcaagg ctgtggaagt gcagaacaag
      1210       1220       1230       1240       1250       1260
cagatgattg aatgggccct cggtggcttc cagccctcgg gtcctaaggg cctggagcca
      1270       1280       1290       1300       1310       1320
ccagaagaag agaagaatcc ttacaaggaa gtttacaccg acatgtgggt ggagcctgaa
      1330       1340       1350       1360       1370       1380
gcagctgctt acgccccacc cccaccagcc aagaaaccca gaaagagcac aacagagaaa
      1390       1400       1410       1420       1430       1440
cctaaggtca aggagatcat tgatgagcgc acaagggagc ggctggtgta tgaggtgcgc
      1450       1460       1470       1480       1490       1500
cagaagtgca gaaacatcga ggacatttgt atctcatgtg ggagcctcaa tgtcaccctg
      1510       1520       1530       1540       1550       1560
gagcacccac tcttcattgg aggcatgtgc cagaactgta agaactgctt cttggagtgt
      1570       1580       1590       1600       1610       1620
gcttaccagt atgacgacga tgggtaccag tcctattgca ccatctgctg tggggggcgt
      1630       1640       1650       1660       1670       1680
gaagtgctca tgtgtgggaa caacaactgc tgcaggtgct tttgtgtcga gtgtgtggat
      1690       1700       1710       1720       1730       1740
ctcttggtgg ggccaggagc tgctcaggca gccattaagg aagacccctg gaactgctac
      1750       1760       1770       1780       1790       1800
atgtgcgggc ataagggcac ctatgggctg ctgcgaagac gggaagactg gccttctcga
      1810       1820       1830       1840       1850       1860
ctccagatgt tctttgccaa taaccatgac caggaatttg accccccaaa ggtttaccca
      1870       1880       1890       1900       1910       1920
cctgtgccag ctgagaagag gaagcccatc cgcgtgctgt ctctctttga tgggattgct
      1930       1940       1950       1960       1970       1980
acagggctcc tggtgctgaa ggacctgggc atccaagtgg accgctacat tgcctccgag
      1990       2000       2010       2020       2030       2040
gtgtgtgagg actccatcac ggtgggcatg gtgcggcacc agggaaagat catgtacgtc
      2050       2060       2070       2080       2090       2100
ggggacgtcc gcagcgtcac acagaagcat atccaggagt ggggcccatt cgacctggtg
      2110       2120       2130       2140       2150       2160
attggaggca gtccctgcaa tgacctctcc attgtcaacc ctgcccgcaa gggactttat
      2170       2180       2190       2200       2210       2220
gagggtactg gccgcctctt ctttgagttc taccgcctcc tgcatgatgc gcggcccaag
      2230       2240       2250       2260       2270       2280
gagggagatg atcgcccctt cttctggctc tttgagaatg tggtggccat gggcgttagt
      2290       2300       2310       2320       2330       2340
gacaagaggg acatctcgcg atttcttgag tctaaccccg tgatgattga cgccaaagaa
      2350       2360       2370       2380       2390       2400
gtgtctgctg cacacagggc ccgttacttc tggggtaacc ttcctggcat gaacaggcct
      2410       2420       2430       2440       2450       2460
ttggcatcca ctgtgaatga taagctggag ctgcaagagt gtctggagca cggcagaata
      2470       2480       2490       2500       2510       2520
gccaagttca gcaaagtgag gaccattacc accaggtcaa actctataaa gcagggcaaa
      2530       2540       2550       2560       2570       2580
gaccagcatt tccccgtctt catgaacgag aaggaggaca tcctgtggtg cactgaaatg
      2590       2600       2610       2620       2630       2640
gaaagggtgt ttggcttccc cgtccactac acagacgtct ccaacatgag ccgcttggcg
      2650       2660       2670       2680       2690       2700
aggcagagac tgctgggccg atcgtggagc gtgccggtca tccgccacct cttcgctccg
      2710       2720
ctgaaggaat attttgcttg tgtgtaa   

Last modification date: Oct. 2, 2011