Mus musculus L. (mouse) [MMU]

FULL NAME: DNA (cytosine-5)-methyltransferase 1


DESCRIPTION:
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

MISCELLANEOUS:
There are three 5' exons, one specific to the oocyte (1c), one specific to the pachytene spermatocyte and also found in skeletal muscle (1b) and one found in somatic cells (1a). Three differents mRNAs can be produced which give rise to two different translation products: isoform 1 (mRNAs-1a) and isoform 2 (mRNA-1b or -1c). Association of DNMT1 with the replication machinery is not strictly required for maintaining global methylation but still enhances methylation efficiency by 2-fold. Pre-existing cytosine methylation at CpG and non-CpG sites enhances methylation activity.


STRUCTURE SIMILARITY:
Belongs to the C5-methyltransferase family.
Contains 1 CXXC-type zinc finger.
Contains 2 BAH domains.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.


ENZYME REGULATION:
Allosterically regulated. The binding of 5-methylcytosine-containing DNA to the N-terminal parts of DNMT1 causes an allosteric activation of the catalytic domain by a direct interaction of its Zn-binding domain with the catalytic domain.


POST-TRANSLATIONAL MODIFICATION:
Sumoylated (By similarity).


PROTEIN TYPE(S):
DNA methyltransferase


RELATED PATHWAY(S):
DNA replication
heterochromatin formation


Amino acids sequence

        10         20         30         40         50         60
MPARTAPARV PALASPAGSL PDHVRRRLKD LERDGLTEKE CVREKLNLLH EFLQTEIKSQ
        70         80         90        100        110        120
LCDLETKLHK EELSEEGYLA KVKSLLNKDL SLENGTHTLT QKANGCPANG SRPTWRAEMA
       130        140        150        160        170        180
DSNRSPRSRP KPRGPRRSKS DSDTLSVETS PSSVATRRTT RQTTITAHFT KGPTKRKPKE
       190        200        210        220        230        240
ESEEGNSAES AAEERDQDKK RRVVDTESGA AAAVEKLEEV TAGTQLGPEE PCEQEDDNRS
       250        260        270        280        290        300
LRRHTRELSL RRKSKEDPDR EARPETHLDE DEDGKKDKRS SRPRSQPRDP AAKRRPKEAE
       310        320        330        340        350        360
PEQVAPETPE DRDEDEREEK RRKTTRKKLE SHTVPVQSRS ERKAAQSKSV IPKINSPKCP
       370        380        390        400        410        420
ECGQHLDDPN LKYQQHPEDA VDEPQMLTSE KLSIYDSTST WFDTYEDSPM HRFTSFSVYC
       430        440        450        460        470        480
SRGHLCPVDT GLIEKNVELY FSGCAKAIHD ENPSMEGGIN GKNLGPINQW WLSGFDGGEK
       490        500        510        520        530        540
VLIGFSTAFA EYILMEPSKE YEPIFGLMQE KIYISKIVVE FLQNNPDAVY EDLINKIETT
       550        560        570        580        590        600
VPPSTINVNR FTEDSLLRHA QFVVSQVESY DEAKDDDETP IFLSPCMRAL IHLAGVSLGQ
       610        620        630        640        650        660
RRATRRVMGA TKEKDKAPTK ATTTKLVYQI FDTFFSEQIE KYDKEDKENA MKRRRCGVCE
       670        680        690        700        710        720
VCQQPECGKC KACKDMVKFG GTGRSKQACL KRRCPNLAVK EADDDEEADD DVSEMPSPKK
       730        740        750        760        770        780
LHQGKKKKQN KDRISWLGQP MKIEENRTYY QKVSIDEEML EVGDCVSVIP DDSSKPLYLA
       790        800        810        820        830        840
RVTALWEDKN GQMMFHAHWF CAGTDTVLGA TSDPLELFLV GECENMQLSY IHSKVKVIYK
       850        860        870        880        890        900
APSENWAMEG GTDPETTLPG AEDGKTYFFQ LWYNQEYARF ESPPKTQPTE DNKHKFCLSC
       910        920        930        940        950        960
IRLAELRQKE MPKVLEQIEE VDGRVYCSSI TKNGVVYRLG DSVYLPPEAF TFNIKVASPV
       970        980        990       1000       1010       1020
KRPKKDPVNE TLYPEHYRKY SDYIKGSNLD APEPYRIGRI KEIHCGKKKG KVNEADIKLR
      1030       1040       1050       1060       1070       1080
LYKFYRPENT HRSYNGSYHT DINMLYWSDE EAVVNFSDVQ GRCTVEYGED LLESIQDYSQ
      1090       1100       1110       1120       1130       1140
GGPDRFYFLE AYNSKTKNFE DPPNHARSPG NKGKGKGKGK GKGKHQVSEP KEPEAAIKLP
      1150       1160       1170       1180       1190       1200
KLRTLDVFSG CGGLSEGFHQ AGISETLWAI EMWDPAAQAF RLNNPGTTVF TEDCNVLLKL
      1210       1220       1230       1240       1250       1260
VMAGEVTNSL GQRLPQKGDV EMLCGGPPCQ GFSGMNRFNS RTYSKFKNSL VVSFLSYCDY
      1270       1280       1290       1300       1310       1320
YRPRFFLLEN VRNFVSYRRS MVLKLTLRCL VRMGYQCTFG VLQAGQYGVA QTRRRAIILA
      1330       1340       1350       1360       1370       1380
AAPGEKLPLF PEPLHVFAPR ACQLSVVVDD KKFVSNITRL SSGPFRTITV RDTMSDLPEI
      1390       1400       1410       1420       1430       1440
QNGASNSEIP YNGEPLSWFQ RQLRGSHYQP ILRDHICKDM SPLVAARMRH IPLFPGSDWR
      1450       1460       1470       1480       1490       1500
DLPNIQVRLG DGVIAHKLQY TFHDVKNGYS STGALRGVCS CAEGKACDPE SRQFSTLIPW
      1510       1520       1530       1540       1550       1560
CLPHTGNRHN HWAGLYGRLE WDGFFSTTVT NPEPMGKQGR VLHPEQHRVV SVRECARSQG
      1570       1580       1590       1600       1610       1620
FPDSYRFFGN ILDRHRQVGN AVPPPLAKAI GLEIKLCLLS SARESASAAV KAKEEAATKD

Encoded by Dnmt1 gene

FULL NAME: DNA methyltransferase (cytosine-5) 1


OTHER NAME(S):
Cxxc9
Dnmt
Dnmt1o
MCMT
MTase
Met-1
Met1
MommeD2
m.MmuI


Nucleic acid sequence

        10         20         30         40         50         60
atgccagcgc gaacagctcc agcccgagtg cctgcgcttg cctccccggc aggctcgctc
        70         80         90        100        110        120
ccggaccatg tccgcaggcg gctcaaagac ttggaaagag atggcttaac agaaaaggag
       130        140        150        160        170        180
tgtgtgaggg agaaattaaa cttactgcat gaattcctgc aaacagaaat aaaaagccag
       190        200        210        220        230        240
ttgtgtgact tggaaaccaa attacataaa gaggaattat ctgaggaagg ctacctggct
       250        260        270        280        290        300
aaagtcaagt ccctcttaaa taaggatttg tccttggaga acggaacaca cactctcact
       310        320        330        340        350        360
caaaaagcca acggttgtcc cgccaacggg agccggccaa cctggagagc agaaatggca
       370        380        390        400        410        420
gactcaaata gatccccaag atccaggccc aagcctcggg gacccaggag aagcaagtcg
       430        440        450        460        470        480
gacagtgaca ccctttcagt tgaaacttca cctagttccg tggctacgag gagaaccacc
       490        500        510        520        530        540
aggcagacca ccatcacggc tcacttcacg aagggcccca ctaaacggaa acccaaggaa
       550        560        570        580        590        600
gagtcggaag aggggaactc ggctgagtcg gctgcagagg agagagacca ggataagaaa
       610        620        630        640        650        660
cgcagagttg tagacacaga gagtggtgct gcagctgctg tggagaaact ggaagaggta
       670        680        690        700        710        720
acagcgggaa cccagctggg tccggaagag ccatgtgaac aggaagatga caacaggagt
       730        740        750        760        770        780
cttcgacgtc acaccagaga gctatcattg aggcggaaat caaaggagga tccagacaga
       790        800        810        820        830        840
gaagcaagac cggaaactca cttggacgag gacgaggacg gaaaaaagga taaaagaagt
       850        860        870        880        890        900
tccagaccca ggagccagcc cagagatcca gctgccaaac ggagacccaa ggaagcagag
       910        920        930        940        950        960
ccagagcagg tagctccaga gactcccgag gacagagacg aggatgagag ggaggagaag
       970        980        990       1000       1010       1020
agacgaaaaa cgacacgtaa aaaactggag tcacacaccg ttcccgttca gagcagatcg
      1030       1040       1050       1060       1070       1080
gagagaaaag ccgctcaaag caaaagtgtg atcccgaaga tcaactcacc aaagtgcccc
      1090       1100       1110       1120       1130       1140
gagtgtggcc agcacctaga cgaccctaac ctgaagtacc agcagcaccc tgaggatgct
      1150       1160       1170       1180       1190       1200
gtggatgaac cccagatgtt gaccagtgag aaactgtcca tctacgactc cacctcgacc
      1210       1220       1230       1240       1250       1260
tggtttgata cttatgaaga ttctcccatg cataggttca cttccttcag tgtgtactgc
      1270       1280       1290       1300       1310       1320
agtcgcgggc acctgtgtcc tgtcgacacc ggtctcattg agaagaatgt agagctctac
      1330       1340       1350       1360       1370       1380
ttttctgggt gtgccaaagc aattcatgac gagaatccat ctatggaagg tggtattaat
      1390       1400       1410       1420       1430       1440
ggcaaaaacc tcgggccaat caatcagtgg tggctcagtg gctttgatgg tggcgagaag
      1450       1460       1470       1480       1490       1500
gtgctcattg gcttctccac tgcatttgct gaatacattt tgatggagcc cagcaaagag
      1510       1520       1530       1540       1550       1560
tatgagccaa tatttgggct gatgcaggag aaaatttaca tcagcaagat tgttgttgag
      1570       1580       1590       1600       1610       1620
ttcctgcaaa acaatcctga tgctgtatat gaagacctga tcaataagat tgagaccact
      1630       1640       1650       1660       1670       1680
gttcctcctt ctaccattaa tgtgaaccgg ttcacagagg actccctctt acgccacgcc
      1690       1700       1710       1720       1730       1740
cagtttgtag tgagccaggt agagagttac gacgaagcca aggacgatga tgagaccccc
      1750       1760       1770       1780       1790       1800
atcttcttgt ctccctgtat gagagccctg atccatttgg ctggtgtctc cctgggacag
      1810       1820       1830       1840       1850       1860
aggcgagcaa caaggcgcgt catgggtgct accaaggaga aggacaaagc acccacgaaa
      1870       1880       1890       1900       1910       1920
gccaccacca ccaagctggt ctatcagatc tttgacactt tcttctcaga gcagattgag
      1930       1940       1950       1960       1970       1980
aagtatgata aggaggacaa ggagaatgcc atgaagcgcc gccgctgtgg tgtctgtgag
      1990       2000       2010       2020       2030       2040
gtctgtcagc agcctgagtg tgggaagtgc aaggcgtgca aagatatggt gaagtttggt
      2050       2060       2070       2080       2090       2100
ggcactggac ggagtaagca ggcttgcctc aagaggaggt gtcctaactt ggcggtgaag
      2110       2120       2130       2140       2150       2160
gaggcagacg acgatgaaga ggctgatgat gatgtgtcag agatgccatc acccaaaaag
      2170       2180       2190       2200       2210       2220
ctgcatcagg ggaagaagaa gaagcagaac aaggaccgca tctcctggct tgggcagcct
      2230       2240       2250       2260       2270       2280
atgaagattg aagagaatag aacttactat cagaaggtga gcatcgatga ggagatgcta
      2290       2300       2310       2320       2330       2340
gaggtgggcg actgcgtctc ggtcattcca gatgattcct ccaaaccact ctatctagcc
      2350       2360       2370       2380       2390       2400
agggtcacag ctctgtggga agacaaaaat ggtcagatga tgttccatgc gcactggttc
      2410       2420       2430       2440       2450       2460
tgcgctggga cagacacagt cctgggagcc acctccgacc ccctggaact gttcctggtg
      2470       2480       2490       2500       2510       2520
ggcgagtgcg aaaacatgca gctttcctac atccacagca aggtcaaggt catctacaaa
      2530       2540       2550       2560       2570       2580
gccccttctg aaaactgggc catggaggga ggcacagacc ctgagaccac actgcctggg
      2590       2600       2610       2620       2630       2640
gctgaggatg gcaagactta cttcttccag ctctggtaca accaggagta cgcaaggttt
      2650       2660       2670       2680       2690       2700
gaatccccac ccaagaccca gccgaccgag gacaacaagc acaagttctg cctatcttgt
      2710       2720       2730       2740       2750       2760
atccggctgg ctgagctgag acaaaaagaa atgcccaagg tcctggaaca aattgaggag
      2770       2780       2790       2800       2810       2820
gtggatggcc gggtctactg cagttccatc accaagaatg gtgttgtcta ccgactgggt
      2830       2840       2850       2860       2870       2880
gacagtgtgt accttcctcc cgaggccttt actttcaaca tcaaagtggc tagccccgtg
      2890       2900       2910       2920       2930       2940
aaacgcccaa agaaggatcc tgtgaacgag accctgtacc ctgagcacta ccgcaagtat
      2950       2960       2970       2980       2990       3000
tctgactaca tcaaggggag caacctggat gctccagagc cctatcgcat cggtcggata
      3010       3020       3030       3040       3050       3060
aaagagatcc actgtggcaa gaagaaaggc aaggtcaacg aggcagacat caagctgagg
      3070       3080       3090       3100       3110       3120
ctctacaagt tctacaggcc tgagaatacc cacaggtcct acaacggatc ctatcacact
      3130       3140       3150       3160       3170       3180
gacatcaaca tgctttactg gagcgacgag gaagctgtgg tgaacttcag cgacgtgcag
      3190       3200       3210       3220       3230       3240
ggccgctgta ccgtggagta cggggaagac ctacttgaga gcatccagga ttattcacaa
      3250       3260       3270       3280       3290       3300
gggggccctg accgcttcta cttcctcgag gcctacaatt caaagaccaa gaactttgaa
      3310       3320       3330       3340       3350       3360
gacccaccaa accatgcccg cagccctggg aacaaaggga aagggaaggg gaaagggaag
      3370       3380       3390       3400       3410       3420
gggaagggga agcatcaggt gtcagagccc aaagagcctg aggcagccat caaactgccc
      3430       3440       3450       3460       3470       3480
aagctccgga ccctggatgt gttttccggc tgtggagggt tatcggaagg attccaccaa
      3490       3500       3510       3520       3530       3540
gcaggcatct cggaaacgct gtgggccatc gagatgtggg acccggcagc ccaggcattt
      3550       3560       3570       3580       3590       3600
cggctgaaca accccggcac cacagtgttc acagaggact gcaacgtgct tcttaagctg
      3610       3620       3630       3640       3650       3660
gtcatggctg gggaggtgac caactctctg ggccaaaggc tgccacagaa gggcgatgtg
      3670       3680       3690       3700       3710       3720
gagatgctgt gtggtgggcc accctgccag ggcttcagtg gcatgaaccg cttcaactcc
      3730       3740       3750       3760       3770       3780
cgcacttact ccaagttcaa aaactcccta gtggtctcct tcctcagcta ctgtgactac
      3790       3800       3810       3820       3830       3840
taccggcctc ggttcttcct tctggagaac gtcaggaact tcgtgtccta cagacgctcc
      3850       3860       3870       3880       3890       3900
atggtgctga agctcacact gcgctgcctg gtccgcatgg gctaccagtg cacctttggt
      3910       3920       3930       3940       3950       3960
gtgctccagg ctggacagta tggcgtggcc cagacacgaa ggagggccat catcttggct
      3970       3980       3990       4000       4010       4020
gcagccccag gagaaaagct gcctctgttc ccagagcctc tgcatgtgtt tgcgccccgt
      4030       4040       4050       4060       4070       4080
gcctgccagc tgagcgttgt ggtggatgac aagaagtttg ttagcaacat aacgaggctg
      4090       4100       4110       4120       4130       4140
agctcggggc ccttccgaac catcaccgtg cgagacacca tgtctgacct ccccgagatc
      4150       4160       4170       4180       4190       4200
cagaatggag cctcgaattc tgagatcccc tacaatggag agccactgtc ctggttccag
      4210       4220       4230       4240       4250       4260
aggcagctgc gaggatcaca ctaccagccc atcctcaggg accatatctg caaggacatg
      4270       4280       4290       4300       4310       4320
agcccactgg tggctgcccg catgcggcac atcccactgt tcccaggatc agattggcgt
      4330       4340       4350       4360       4370       4380
gacctgccca acatacaggt gcggctggga gatggcgtca tagcccataa gctacagtac
      4390       4400       4410       4420       4430       4440
acctttcatg atgtgaaaaa tggctacagc agcaccggtg ccctgcgtgg agtctgttcc
      4450       4460       4470       4480       4490       4500
tgtgcagaag gcaaggcctg cgaccctgag tccaggcaat tcagcaccct catcccctgg
      4510       4520       4530       4540       4550       4560
tgcctgccgc acactgggaa ccggcacaac cactgggctg gcctctacgg gcgtctggag
      4570       4580       4590       4600       4610       4620
tgggatggct tcttcagcac cactgtcacc aaccctgagc ccatgggcaa gcagggtcgg
      4630       4640       4650       4660       4670       4680
gtgctccacc cggagcagca ccgggtcgtg agtgttcggg aatgtgcccg ctcccagggc
      4690       4700       4710       4720       4730       4740
tttccagata gctaccggtt cttcggcaac atcctggaca gacaccggca ggtgggtaat
      4750       4760       4770       4780       4790       4800
gctgtgccac cacccctggc caaagccatt ggcctggaga ttaagctctg cctgctgtcc
      4810       4820       4830       4840       4850       4860
agtgctcggg agagcgcatc agctgcagtt aaagcaaaag aggaggctgc taccaaggac

tag     

Last modification date: Oct. 2, 2011