Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA replication licensing factor MCM6


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Required for the entry in S phase and for cell division.

MISCELLANEOUS:
Present with 13400 molecules/cell in log phase SD medium.
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSSPFPADTP SSNRPSNSSP PPSSIGAGFG SSSGLDSQIG SRLHFPSSSQ PHVSNSQTGP
        70         80         90        100        110        120
FVNDSTQFSS QRLQTDGSAT NDMEGNEPAR SFKSRALNHV KKVDDVTGEK VREAFEQFLE
       130        140        150        160        170        180
DFSVQSTDTG EVEKVYRAQI EFMKIYDLNT IYIDYQHLSM RENGALAMAI SEQYYRFLPF
       190        200        210        220        230        240
LQKGLRRVVR KYAPELLNTS DSLKRSEGDE GQADEDEQQD DDMNGSSLPR DSGSSAAPGN
       250        260        270        280        290        300
GTSAMATRSI TTSTSPEQTE RVFQISFFNL PTVHRIRDIR SEKIGSLLSI SGTVTRTSEV
       310        320        330        340        350        360
RPELYKASFT CDMCRAIVDN VEQSFKYTEP TFCPNPSCEN RAFWTLNVTR SRFLDWQKVR
       370        380        390        400        410        420
IQENANEIPT GSMPRTLDVI LRGDSVERAK PGDRCKFTGV EIVVPDVTQL GLPGVKPSST
       430        440        450        460        470        480
LDTRGISKTT EGLNSGVTGL RSLGVRDLTY KISFLACHVI SIGSNIGASS PDANSNNRET
       490        500        510        520        530        540
ELQMAANLQA NNVYQDNERD QEVFLNSLSS DEINELKEMV KDEHIYDKLV RSIAPAVFGH
       550        560        570        580        590        600
EAVKKGILLQ MLGGVHKSTV EGIKLRGDIN ICVVGDPSTS KSQFLKYVVG FAPRSVYTSG
       610        620        630        640        650        660
KASSAAGLTA AVVRDEEGGD YTIEAGALML ADNGICCIDE FDKMDISDQV AIHEAMEQQT
       670        680        690        700        710        720
ISIAKAGIHA TLNARTSILA AANPVGGRYN RKLSLRGNLN MTAPIMSRFD LFFVILDDCN
       730        740        750        760        770        780
EKIDTELASH IVDLHMKRDE AIEPPFSAEQ LRRYIKYART FKPILTKEAR SYLVEKYKEL
       790        800        810        820        830        840
RKDDAQGFSR SSYRITVRQL ESMIRLSEAI ARANCVDEIT PSFIAEAYDL LRQSIIRVDV
       850        860        870        880        890        900
DDVEMDEEFD NIESQSHAAS GNNDDNDDGT GSGVITSEPP ADIEEGQSEA TARPGTSEKK
       910        920        930        940        950        960
KTTVTYDKYV SMMNMIVRKI AEVDREGAEE LTAVDIVDWY LLQKENDLGS LAEYWEERRL
       970        980        990       1000       1010
AFKVIKRLVK DRILMEIHGT RHNLRDLENE ENENNKTVYV IHPNCEVLDQ LEPQDSS

Encoded by MCM6 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtcatccc cttttccagc tgacacacca agcagtaata gaccttccaa ctcctctccg
        70         80         90        100        110        120
ccaccatcgt cgataggtgc tggcttcgga agcagtagcg gactcgattc gcaaataggc
       130        140        150        160        170        180
tctaggttac atttcccaag ttcttctcaa ccgcatgtca gtaattcaca aacaggccct
       190        200        210        220        230        240
tttgttaacg attccactca gtttagttct caaaggttgc aaaccgatgg ttccgcgact
       250        260        270        280        290        300
aatgatatgg agggaaatga acctgccaga agcttcaaaa gtagagcttt gaatcacgtt
       310        320        330        340        350        360
aagaaagttg atgacgttac tggtgaaaaa gtccgtgaag cgttcgagca gtttttggaa
       370        380        390        400        410        420
gacttttccg ttcaatccac tgatactggt gaagttgaaa aagtttatag agctcaaatt
       430        440        450        460        470        480
gaatttatga aaatatatga cctaaacacc atttatatag attatcagca tttatccatg
       490        500        510        520        530        540
agagaaaatg gtgctctagc tatggctatc tcagaacaat attacagatt tttgcctttt
       550        560        570        580        590        600
ttacaaaagg gtttaagaag agtagtaaga aagtatgcac ctgaattgtt aaacacaagt
       610        620        630        640        650        660
gactcattga agagatccga aggtgacgaa ggtcaagcgg atgaagatga gcaacaggac
       670        680        690        700        710        720
gatgatatga atgggtcgag tctaccaaga gattctggat catcagccgc accagggaat
       730        740        750        760        770        780
gggacatctg ccatggcaac gaggtcaata acaacttcta cgtcacctga acagacggaa
       790        800        810        820        830        840
cgtgtttttc aaatcagttt cttcaatcta ccaacagttc acagaattcg tgatataaga
       850        860        870        880        890        900
tctgaaaaga ttggttcact attgagtata tcagggacag tgacaagaac atctgaggtc
       910        920        930        940        950        960
cgtccagaat tatacaaggc aagctttacg tgtgatatgt gccgtgctat cgtagataac
       970        980        990       1000       1010       1020
gtagaacagt ccttcaagta cactgaaccg acattttgcc cgaacccatc atgcgaaaat
      1030       1040       1050       1060       1070       1080
agagcctttt ggacattaaa tgttacaaga tccaggtttc ttgattggca aaaagtcaga
      1090       1100       1110       1120       1130       1140
attcaagaaa atgctaacga aatacctaca ggctccatgc cgcgtacgct ggacgtcatt
      1150       1160       1170       1180       1190       1200
cttcgaggtg atagtgtcga aagagccaag ccaggtgacc gttgcaaatt tacgggtgtg
      1210       1220       1230       1240       1250       1260
gaaattgtag tacctgatgt tacacaattg gggctaccag gtgtgaagcc aagttcaaca
      1270       1280       1290       1300       1310       1320
ttagatacta gaggtatttc gaagactact gaaggtttga atagtggtgt tactggttta
      1330       1340       1350       1360       1370       1380
cgctctcttg gtgttcgtga tttgacatac aagattagct ttctggcatg ccatgttatc
      1390       1400       1410       1420       1430       1440
agtattggat caaacattgg tgcaagtagc cctgatgcaa attctaacaa ccgagaaact
      1450       1460       1470       1480       1490       1500
gaactacaga tggctgctaa tttacaagct aataatgtat accaagataa tgaaagagat
      1510       1520       1530       1540       1550       1560
caagaagtat tcttgaacag tttgagttca gatgaaataa atgagctgaa ggaaatggta
      1570       1580       1590       1600       1610       1620
aaggatgaac atatttatga taaattggtc agatctattg ctccagctgt gtttggccat
      1630       1640       1650       1660       1670       1680
gaagccgtaa agaagggtat cttgcttcag atgctcggtg gtgttcataa gagtactgta
      1690       1700       1710       1720       1730       1740
gaaggtatta agttaagagg agatatcaac atctgtgttg ttggtgatcc ctctacttct
      1750       1760       1770       1780       1790       1800
aagtctcaat ttttaaaata cgtagtggga tttgcaccaa gatcagttta cacttccggt
      1810       1820       1830       1840       1850       1860
aaggcctcgt cggctgctgg tttaacagct gcggttgtga gagatgagga aggaggtgat
      1870       1880       1890       1900       1910       1920
tatacaattg aagcaggtgc tttaatgctt gctgataacg gtatttgttg tatcgatgaa
      1930       1940       1950       1960       1970       1980
tttgataaaa tggatatttc agatcaagtt gccattcatg aagctatgga acaacagacc
      1990       2000       2010       2020       2030       2040
atctctattg ctaaagctgg tattcacgct actttaaatg caagaacatc aattctagcg
      2050       2060       2070       2080       2090       2100
gctgccaacc cggtaggtgg aagatacaat aggaaactat cactgagggg taatttaaat
      2110       2120       2130       2140       2150       2160
atgaccgcac cgatcatgtc cagatttgat ttattttttg ttattcttga tgactgtaac
      2170       2180       2190       2200       2210       2220
gaaaaaattg ataccgaatt ggcatctcat atcgttgact tacacatgaa aagagacgaa
      2230       2240       2250       2260       2270       2280
gccattgagc cgccatttag tgcagaacaa ctacgtcgtt atatcaaata tgcacgtact
      2290       2300       2310       2320       2330       2340
tttaaaccaa tattaacgaa agaagcccgt tcctatttag tcgagaagta taaggaactg
      2350       2360       2370       2380       2390       2400
agaaaggatg atgcccaggg atttagtaga tcgagttata gaatcacagt taggcaactt
      2410       2420       2430       2440       2450       2460
gaaagtatga ttagattatc agaagctatt gcgagggcta attgtgtcga tgaaattact
      2470       2480       2490       2500       2510       2520
ccatcattca ttgcagaagc ttacgatctt ttaaggcaaa gtattattcg tgttgatgtg
      2530       2540       2550       2560       2570       2580
gatgatgtgg aaatggatga agaatttgat aacatagaga gtcaaagtca cgccgcttct
      2590       2600       2610       2620       2630       2640
ggaaacaatg atgacaatga tgatgggact gggtcaggtg taattacgag tgagccacca
      2650       2660       2670       2680       2690       2700
gcagatatag aagaaggaca aagcgaagct acagctcgtc caggtacaag cgagaagaag
      2710       2720       2730       2740       2750       2760
aaaacaacgg tgacgtatga taaatatgtg tccatgatga acatgatcgt tagaaagatt
      2770       2780       2790       2800       2810       2820
gctgaagtag atagggaggg tgcagaagaa ctaacagctg tggatatagt tgattggtat
      2830       2840       2850       2860       2870       2880
ttattacaga aggaaaatga tttgggttcg ttagcagaat attgggaaga gagaagatta
      2890       2900       2910       2920       2930       2940
gcgttcaaag tcataaaaag gttggtcaaa gataggattt taatggagat tcatggtacc
      2950       2960       2970       2980       2990       3000
agacacaatt taagagatct tgagaatgag gaaaatgaaa ataataaaac tgtttatgtt
      3010       3020       3030       3040       3050
attcatccaa actgtgaggt tttggatcaa ttggaaccac aggattccag ctaa

Last modification date: Oct. 2, 2011