Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA polymerase II


DESCRIPTION:
DNA polymerase II is a specialized repair enzyme. Like Pol I, a large number of Pol II molecules reside in the cell (about 100). The enzyme is more processive than Pol I. Pol II has the same editing (3′ to 5′) activity as Pol I, but not the 5′ to 3′ exonuclease activity. Thought to be involved in DNA repair and/or mutagenesis.

STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-B family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


ENZYME REGULATION:
DNA polymerase II activity is regulated by the lexA gene during the SOS response.


PROTEIN TYPE(S):
B-family DNA polymerase
3'-5' exonuclease


RELATED PATHWAY(S):
translesion synthesis (TLS)
prokaryotic (SOS) response


Amino acids sequence

        10         20         30         40         50         60
MAQAGFILTR HWRDTPQGTE VSFWLATDNG PLQVTLAPQE SVAFIPADQV PRAQHILQGE
        70         80         90        100        110        120
QGFRLTPLAL KDFHRQPVYG LYCRAHRQLM NYEKRLREGG VTVYEADVRP PERYLMERFI
       130        140        150        160        170        180
TSPVWVEGDM HNGTIVNARL KPHPDYRPPL KWVSIDIETT RHGELYCIGL EGCGQRIVYM
       190        200        210        220        230        240
LGPENGDASS LDFELEYVAS RPQLLEKLNA WFANYDPDVI IGWNVVQFDL RMLQKHAERY
       250        260        270        280        290        300
RLPLRLGRDN SELEWREHGF KNGVFFAQAK GRLIIDGIEA LKSAFWNFSS FSLETVAQEL
       310        320        330        340        350        360
LGEGKSIDNP WDRMDEIDRR FAEDKPALAT YNLKDCELVT QIFHKTEIMP FLLERATVNG
       370        380        390        400        410        420
LPVDRHGGSV AAFGHLYFPR MHRAGYVAPN LGEVPPHASP GGYVMDSRPG LYDSVLVLDY
       430        440        450        460        470        480
KSLYPSIIRT FLIDPVGLVE GMAQPDPEHS TEGFLDAWFS REKHCLPEIV TNIWHGRDEA
       490        500        510        520        530        540
KRQGNKPLSQ ALKIIMNAFY GVLGTTACRF FDPRLASSIT MRGHQIMRQT KALIEAQGYD
       550        560        570        580        590        600
VIYGDTDSTF VWLKGAHSEE EAAKIGRALV QHVNAWWAET LQKQRLTSAL ELEYETHFCR
       610        620        630        640        650        660
FLMPTIRGAD TGSKKRYAGL IQEGDKQRMV FKGLETVRTD WTPLAQQFQQ ELYLRIFRNE
       670        680        690        700        710        720
PYQEYVRETI DKLMAGELDA RLVYRKRLRR PLSEYQRNVP PHVRAARLAD EENQKRGRPL
       730        740        750        760        770        780
QYQNRGTIKY VWTTNGPEPL DYQRSPLDYE HYLTRQLQPV AEGILPFIED NFATLMTGQL

GLF     

Encoded by polB gene


OTHER NAME(S):
dinA
ECK0061
JW0059


DESCRIPTION:
DNA polymerase II (Pol II) is a combined polymerase and exonuclease involved in replication restart following UV exposure, translesion synthesis and nucleotide excision repair. [More information is available at EcoCyc: EG10747].


Nucleic acid sequence

        10         20         30         40         50         60
gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca agggacagaa
        70         80         90        100        110        120
gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc accgcaagag
       130        140        150        160        170        180
tccgtggcgt ttattcccgc cgatcaggtt ccccgcgctc agcatatttt gcagggtgaa
       190        200        210        220        230        240
caaggctttc gcctgacacc gctggcgtta aaggattttc accgccagcc ggtgtatggc
       250        260        270        280        290        300
ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgcctgcg tgaaggtggc
       310        320        330        340        350        360
gttaccgtct acgaggccga tgtgcgtccg ccagaacgct atctgatgga gcggtttatc
       370        380        390        400        410        420
acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa tgcccgtctg
       430        440        450        460        470        480
aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat tgaaaccacc
       490        500        510        520        530        540
cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat cgtttatatg
       550        560        570        580        590        600
ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata cgtcgccagc
       610        620        630        640        650        660
cgcccgcagt tgctggaaaa actcaacgcc tggtttgcca actacgatcc tgatgtgatc
       670        680        690        700        710        720
atcggttgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc cgagcgttac
       730        740        750        760        770        780
cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga gcacggcttt
       790        800        810        820        830        840
aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg tatcgaggcg
       850        860        870        880        890        900
ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc tcaggagcta
       910        920        930        940        950        960
ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat tgaccgccgt
       970        980        990       1000       1010       1020
ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga gctggtgacg
      1030       1040       1050       1060       1070       1080
cagatcttcc acaaaactga aatcatgcca tttttactcg aacgggcaac ggtgaacggc
      1090       1100       1110       1120       1130       1140
ctgccggtgg accgacacgg cggttcggtg gcggcatttg gtcatctcta ttttccgcga
      1150       1160       1170       1180       1190       1200
atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca cgccagccct
      1210       1220       1230       1240       1250       1260
ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt gctggactat
      1270       1280       1290       1300       1310       1320
aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg gctggtggaa
      1330       1340       1350       1360       1370       1380
ggcatggcgc agcctgatcc agagcacagt accgaaggtt ttctcgatgc ctggttctcg
      1390       1400       1410       1420       1430       1440
cgagaaaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg cgatgaagcc
      1450       1460       1470       1480       1490       1500
aaacgccagg gtaacaaacc gctgtcgcag gcgctgaaaa tcatcatgaa tgccttttat
      1510       1520       1530       1540       1550       1560
ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc ggctggcatc gtcgatcacc
      1570       1580       1590       1600       1610       1620
atgcgtggtc atcagatcat gcggcaaacc aaagcgttga ttgaagcaca gggctacgac
      1630       1640       1650       1660       1670       1680
gttatctacg gcgataccga ctcaacgttt gtctggctga aaggcgcaca ttcggaagaa
      1690       1700       1710       1720       1730       1740
gaagcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg ggcggaaacg
      1750       1760       1770       1780       1790       1800
ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaaccca tttctgccgt
      1810       1820       1830       1840       1850       1860
tttctgatgc caaccattcg cggagccgat accggcagta aaaagcgtta tgccggactg
      1870       1880       1890       1900       1910       1920
attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt gcgcaccgac
      1930       1940       1950       1960       1970       1980
tggacgccgc tggcccagca gtttcagcag gagctatacc tgcgcatctt ccgcaacgag
      1990       2000       2010       2020       2030       2040
ccatatcagg aatatgtacg cgaaaccatc gacaaactga tggcgggtga actggatgcg
      2050       2060       2070       2080       2090       2100
cgactggttt accgtaaacg ccttcgccgt ccgctgagcg agtatcagcg taatgtgccg
      2110       2120       2130       2140       2150       2160
cctcatgtac gcgccgctcg ccttgccgat gaagaaaacc aaaagcgtgg tcgccccttg
      2170       2180       2190       2200       2210       2220
caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc ggagccgctg
      2230       2240       2250       2260       2270       2280
gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct acaacccgtg
      2290       2300       2310       2320       2330       2340
gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac cgggcaactt
      2350
gggctatttt ga    

Last modification date: Oct. 7, 2011