Homo sapiens L. (human) [HSA]

FULL NAME: DNA topoisomerase 2-beta


DESCRIPTION:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Indirectly ivolved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
drug target:
Podophyllotoxin: D05529
Etoposide: D00125 D04107
Teniposide: D02698
Doxorubicin: D01275 D03899
Daunorubicin: D01264 D07776
Epirubicin: D02214 D07901
Aclarubicin: D01911 D02756
Idarubicin: D01747 D08062
Mitoxantrone: D02166 D08224
Pirarubicin: D01885 D08386
Amrubicin: D07100 D08854
Amsacrine: D02321
Other: D01404 D03602 D04498 D04685 D04791 D06545

MISCELLANEOUS:
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the type II topoisomerase family.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR.


PROTEIN TYPE(S):
endonuclease
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAKSGGCGAG AGVGGGNGAL TWVTLFDQNN AAKKEESETA NKNDSSKKLS VERVYQKKTQ
        70         80         90        100        110        120
LEHILLRPDT YIGSVEPLTQ FMWVYDEDVG MNCREVTFVP GLYKIFDEIL VNAADNKQRD
       130        140        150        160        170        180
KNMTCIKVSI DPESNIISIW NNGKGIPVVE HKVEKVYVPA LIFGQLLTSS NYDDDEKKVT
       190        200        210        220        230        240
GGRNGYGAKL CNIFSTKFTV ETACKEYKHS FKQTWMNNMM KTSEAKIKHF DGEDYTCITF
       250        260        270        280        290        300
QPDLSKFKME KLDKDIVALM TRRAYDLAGS CRGVKVMFNG KKLPVNGFRS YVDLYVKDKL
       310        320        330        340        350        360
DETGVALKVI HELANERWDV CLTLSEKGFQ QISFVNSIAT TKGGRHVDYV VDQVVGKLIE
       370        380        390        400        410        420
VVKKKNKAGV SVKPFQVKNH IWVFINCLIE NPTFDSQTKE NMTLQPKSFG SKCQLSEKFF
       430        440        450        460        470        480
KAASNCGIVE SILNWVKFKA QTQLNKKCSS VKYSKIKGIP KLDDANDAGG KHSLECTLIL
       490        500        510        520        530        540
TEGDSAKSLA VSGLGVIGRD RYGVFPLRGK ILNVREASHK QIMENAEINN IIKIVGLQYK
       550        560        570        580        590        600
KSYDDAESLK TLRYGKIMIM TDQDQDGSHI KGLLINFIHH NWPSLLKHGF LEEFITPIVK
       610        620        630        640        650        660
ASKNKQELSF YSIPEFDEWK KHIENQKAWK IKYYKGLGTS TAKEAKEYFA DMERHRILFR
       670        680        690        700        710        720
YAGPEDDAAI TLAFSKKKID DRKEWLTNFM EDRRQRRLHG LPEQFLYGTA TKHLTYNDFI
       730        740        750        760        770        780
NKELILFSNS DNERSIPSLV DGFKPGQRKV LFTCFKRNDK REVKVAQLAG SVAEMSAYHH
       790        800        810        820        830        840
GEQALMMTIV NLAQNFVGSN NINLLQPIGQ FGTRLHGGKD AASPRYIFTM LSTLARLLFP
       850        860        870        880        890        900
AVDDNLLKFL YDDNQRVEPE WYIPIIPMVL INGAEGIGTG WACKLPNYDA REIVNNVRRM
       910        920        930        940        950        960
LDGLDPHPML PNYKNFKGTI QELGQNQYAV SGEIFVVDRN TVEITELPVR TWTQVYKEQV
       970        980        990       1000       1010       1020
LEPMLNGTDK TPALISDYKE YHTDTTVKFV VKMTEEKLAQ AEAAGLHKVF KLQTTLTCNS
      1030       1040       1050       1060       1070       1080
MVLFDHMGCL KKYETVQDIL KEFFDLRLSY YGLRKEWLVG MLGAESTKLN NQARFILEKI
      1090       1100       1110       1120       1130       1140
QGKITIENRS KKDLIQMLVQ RGYESDPVKA WKEAQEKAAE EDETQNQHDD SSSDSGTPSG
      1150       1160       1170       1180       1190       1200
PDFNYILNMS LWSLTKEKVE ELIKQRDAKG REVNDLKRKS PSDLWKEDLA AFVEELDKVE
      1210       1220       1230       1240       1250       1260
SQEREDVLAG MSGKAIKGKV GKPKVKKLQL EETMPSPYGR RIIPEITAMK ADASKKLLKK
      1270       1280       1290       1300       1310       1320
KKGDLDTAAV KVEFDEEFSG APVEGAGEEA LTPSVPINKG PKPKREKKEP GTRVRKTPTS
      1330       1340       1350       1360       1370       1380
SGKPSAKKVK KRNPWSDDES KSESDLEETE PVVIPRDSLL RRAAAERPKY TFDFSEEEDD
      1390       1400       1410       1420       1430       1440
DADDDDDDNN DLEELKVKAS PITNDGEDEF VPSDGLDKDE YTFSPGKSKA TPEKSLHDKK
      1450       1460       1470       1480       1490       1500
SQDFGNLFSF PSYSQKSEDD SAKFDSNEED SASVFSPSFG LKQTDKVPSK TVAAKKGKPS
      1510       1520       1530       1540       1550       1560
SDTVPKPKRA PKQKKVVEAV NSDSDSEFGI PKKTTTPKGK GRGAKKRKAS GSENEGDYNP
      1570       1580       1590       1600       1610       1620
GRKTSKTTSK KPKKTSFDQD SDVDIFPSDF PTEPPSLPRT GRARKEVKYF AESDEEEDDV

DFAMFN     

Encoded by TOP2B gene

FULL NAME: topoisomerase (DNA) II beta 180kDa


OTHER NAME(S):
TOPIIB
top2beta


DESCRIPTION:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants; however, the second variant has not yet been fully described. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggccaagt cgggtggctg cggcgcggga gccggcgtgg gcggcggcaa cggggcactg
        70         80         90        100        110        120
acctgggtga acaatgctgc aaaaaaagaa gagtcagaaa ctgccaacaa aaatgattct
       130        140        150        160        170        180
tcaaagaagt tgtctgttga gagagtgtat cagaagaaga cacaacttga acacattctt
       190        200        210        220        230        240
cttcgtcctg atacatatat tgggtcagtg gagccattga cgcagttcat gtgggtgtat
       250        260        270        280        290        300
gatgaagatg taggaatgaa ttgcagggag gttacctttg tgccaggttt atacaagatc
       310        320        330        340        350        360
tttgatgaaa ttttggttaa tgctgctgac aataaacaga gggataagaa catgacttgt
       370        380        390        400        410        420
attaaagttt ctattgatcc tgaatctaac attataagca tttggaataa tgggaaaggc
       430        440        450        460        470        480
attccagtag tagaacacaa ggtagagaaa gtttatgttc ctgctttaat ttttggacag
       490        500        510        520        530        540
cttttaacat ccagtaacta tgatgatgat gagaaaaaag ttacaggtgg tcgtaatggt
       550        560        570        580        590        600
tatggtgcaa aactttgtaa tattttcagt acaaagttta cagtagaaac agcttgcaaa
       610        620        630        640        650        660
gaatacaaac acagttttaa gcagacatgg atgaataata tgatgaagac ttctgaagcc
       670        680        690        700        710        720
aaaattaaac attttgatgg tgaagattac acatgcataa cattccaacc agatctgtcc
       730        740        750        760        770        780
aaatttaaga tggaaaaact tgacaaggat attgtggccc tcatgactag aagggcatat
       790        800        810        820        830        840
gatttggctg gttcgtgtag aggggtcaag gtcatgttta atggaaagaa attgcctgta
       850        860        870        880        890        900
aatggatttc gcagttatgt agatctttat gtgaaagaca aattggatga aactggggtg
       910        920        930        940        950        960
gccctgaaag ttattcatga gcttgcaaat gaaagatggg atgtttgtct cacattgagt
       970        980        990       1000       1010       1020
gaaaaaggat tccagcaaat cagctttgta aatagtattg caactacaaa aggtggacgg
      1030       1040       1050       1060       1070       1080
cacgtggatt atgtggtaga tcaagttgtt ggtaaactga ttgaagtagt taagaaaaag
      1090       1100       1110       1120       1130       1140
aacaaagctg gtgtatcagt gaaaccattt caagtaaaaa accatatatg ggtttttatt
      1150       1160       1170       1180       1190       1200
aattgcctta ttgaaaatcc aacttttgat tctcagacta aggaaaacat gactctgcag
      1210       1220       1230       1240       1250       1260
cccaaaagtt ttgggtctaa atgccagctg tcagaaaaat tttttaaagc agcctctaat
      1270       1280       1290       1300       1310       1320
tgtggcattg tagaaagtat cctgaactgg gtgaaattta aggctcagac tcagctgaat
      1330       1340       1350       1360       1370       1380
aagaagtgtt catcagtaaa atacagtaaa atcaaaggta ttcccaaact ggatgatgct
      1390       1400       1410       1420       1430       1440
aatgatgctg gtggtaaaca ttccctggag tgtacactga tattaacaga gggagactct
      1450       1460       1470       1480       1490       1500
gccaaatcac tggctgtgtc tggattaggt gtgattggac gagacagata cggagttttt
      1510       1520       1530       1540       1550       1560
ccactcaggg gcaaaattct taatgtacgg gaagcttctc ataaacagat catggaaaat
      1570       1580       1590       1600       1610       1620
gctgaaataa ataatattat taaaatagtt ggtctacaat ataagaaaag ttacgatgat
      1630       1640       1650       1660       1670       1680
gcagaatctc tgaaaacctt acgctatgga aagattatga ttatgaccga tcaggatcaa
      1690       1700       1710       1720       1730       1740
gatggttctc acataaaagg cctgcttatt aatttcatcc atcacaattg gccatcactt
      1750       1760       1770       1780       1790       1800
ttgaagcatg gttttcttga agagttcatt actcctattg taaaggcaag caaaaataag
      1810       1820       1830       1840       1850       1860
caggaacttt ccttctacag tattcctgaa tttgacgaat ggaaaaaaca tatagaaaac
      1870       1880       1890       1900       1910       1920
cagaaagcct ggaaaataaa gtactataaa ggattgggta ctagtacagc taaagaagca
      1930       1940       1950       1960       1970       1980
aaggaatatt ttgctgatat ggaaaggcat cgcatcttgt ttagatatgc tggtcctgaa
      1990       2000       2010       2020       2030       2040
gatgatgctg ccattacctt ggcatttagt aagaagaaga ttgatgacag aaaagaatgg
      2050       2060       2070       2080       2090       2100
ttaacaaatt ttatggaaga ccggagacag cgtaggctac atggcttacc agagcaattt
      2110       2120       2130       2140       2150       2160
ttatatggta ctgcaacaaa gcatttgact tataatgatt tcatcaacaa ggaattgatt
      2170       2180       2190       2200       2210       2220
ctcttctcaa actcagacaa tgaaagatct ataccatctc ttgttgatgg ctttaaacct
      2230       2240       2250       2260       2270       2280
ggccagcgga aagttttatt tacctgtttc aagaggaatg ataaacgtga agtaaaagtt
      2290       2300       2310       2320       2330       2340
gcccagttgg ctggctctgt tgctgagatg tcggcttatc atcatggaga acaagcattg
      2350       2360       2370       2380       2390       2400
atgatgacta ttgtgaattt ggctcagaac tttgtgggaa gtaacaacat taacttgctt
      2410       2420       2430       2440       2450       2460
cagcctattg gtcagtttgg aactcggctt catggtggca aagatgctgc aagccctcgt
      2470       2480       2490       2500       2510       2520
tatattttca caatgttaag cactttagca aggctacttt ttcctgctgt ggatgacaac
      2530       2540       2550       2560       2570       2580
ctccttaagt tcctttatga tgataatcaa cgtgtagagc ctgagtggta tattcctata
      2590       2600       2610       2620       2630       2640
attcccatgg ttttaataaa tggtgctgag ggcattggta ctggatgggc ttgtaaacta
      2650       2660       2670       2680       2690       2700
cccaactatg atgctaggga aattgtgaac aatgtcagac gaatgctaga tggcctggat
      2710       2720       2730       2740       2750       2760
cctcatccca tgcttccaaa ctacaaaaac tttaaaggca cgattcaaga acttggtcaa
      2770       2780       2790       2800       2810       2820
aaccagtatg cagtcagtgg tgaaatattt gtagtggaca gaaacacagt agaaattaca
      2830       2840       2850       2860       2870       2880
gagcttccag ttagaacttg gacacaggta tataaagaac aggttttaga acctatgcta
      2890       2900       2910       2920       2930       2940
aatggaacag ataaaacacc agcattaatt tctgattata aagaatatca tactgacaca
      2950       2960       2970       2980       2990       3000
actgtgaaat ttgtggtgaa aatgactgaa gagaaactag cacaagcaga agctgctgga
      3010       3020       3030       3040       3050       3060
ctgcataaag tttttaaact tcaaactact cttacttgta attccatggt actttttgat
      3070       3080       3090       3100       3110       3120
catatgggat gtctgaagaa atatgaaact gtgcaagaca ttctgaaaga attctttgat
      3130       3140       3150       3160       3170       3180
ttacgattaa gttattacgg tttacgtaag gagtggcttg tgggaatgtt gggagcagaa
      3190       3200       3210       3220       3230       3240
tctacaaagc ttaacaatca agcccgtttc attttagaga agatacaagg gaaaattact
      3250       3260       3270       3280       3290       3300
atagagaata ggtcaaagaa agatttgatt caaatgttag tccagagagg ttatgaatct
      3310       3320       3330       3340       3350       3360
gacccagtga aagcctggaa agaagcacaa gaaaaggcag cagaagagga tgaaacacaa
      3370       3380       3390       3400       3410       3420
aaccagcatg atgatagttc ctccgattca ggaactcctt caggcccaga ttttaattat
      3430       3440       3450       3460       3470       3480
attttaaata tgtctctgtg gtctcttact aaagaaaaag ttgaagaact gattaaacag
      3490       3500       3510       3520       3530       3540
agagatgcaa aagggcgaga ggtcaatgat cttaaaagaa aatctccttc agatctttgg
      3550       3560       3570       3580       3590       3600
aaagaggatt tagcggcatt tgttgaagaa ctggataaag tggaatctca agaacgagaa
      3610       3620       3630       3640       3650       3660
gatgttctgg ctggaatgtc tggaaaagca attaaaggta aagttggcaa acctaaggtg
      3670       3680       3690       3700       3710       3720
aagaaactcc agttggaaga gacaatgccc tcaccttatg gcagaagaat aattcctgaa
      3730       3740       3750       3760       3770       3780
attacagcta tgaaggcaga tgccagcaaa aagttgctga agaagaagaa gggtgatctt
      3790       3800       3810       3820       3830       3840
gatactgcag cagtaaaagt ggaatttgat gaagaattca gtggagcacc agtagaaggt
      3850       3860       3870       3880       3890       3900
gcaggagaag aggcattgac tccatcagtt cctataaata aaggtcccaa acctaagagg
      3910       3920       3930       3940       3950       3960
gagaagaagg agcctggtac cagagtgaga aaaacaccta catcatctgg taaacctagt
      3970       3980       3990       4000       4010       4020
gcaaagaaag tgaagaaacg gaatccttgg tcagatgatg aatccaagtc agaaagtgat
      4030       4040       4050       4060       4070       4080
ttggaagaaa cagaacctgt ggttattcca agagattctt tgcttaggag agcagcagcc
      4090       4100       4110       4120       4130       4140
gaaagaccta aatacacatt tgatttctca gaagaagagg atgatgatgc tgatgatgat
      4150       4160       4170       4180       4190       4200
gatgatgaca ataatgattt agaggaattg aaagttaaag catctcccat aacaaatgat
      4210       4220       4230       4240       4250       4260
ggggaagatg aatttgttcc ttcagatggg ttagataaag atgaatatac attttcacca
      4270       4280       4290       4300       4310       4320
ggcaaatcaa aagccactcc agaaaaatct ttgcatgaca aaaaaagtca ggattttgga
      4330       4340       4350       4360       4370       4380
aatctcttct catttccttc atattctcag aagtcagaag atgattcagc taaatttgac
      4390       4400       4410       4420       4430       4440
agtaatgaag aagattctgc ttctgttttt tcaccatcat ttggtctgaa acagacagat
      4450       4460       4470       4480       4490       4500
aaagttccaa gtaaaacggt agctgctaaa aagggaaaac cgtcttcaga tacagtccct
      4510       4520       4530       4540       4550       4560
aagcccaaga gagccccaaa acagaagaaa gtagtagagg ctgtaaactc tgactcggat
      4570       4580       4590       4600       4610       4620
tcagaatttg gcattccaaa gaagactaca acaccaaaag gtaaaggccg aggggcaaag
      4630       4640       4650       4660       4670       4680
aaaaggaaag catctggctc tgaaaatgaa ggcgattata accctggcag gaaaacatcc
      4690       4700       4710       4720       4730       4740
aaaacaacaa gcaagaaacc gaagaagaca tcttttgatc aggattcaga tgtggacatc
      4750       4760       4770       4780       4790       4800
ttcccctcag acttccctac tgagccacct tctctgccac gaaccggtcg ggctaggaaa
      4810       4820       4830       4840       4850       4860
gaagtaaaat attttgcaga gtctgatgaa gaagaagatg atgttgattt tgcaatgttt

aattaa     

Last modification date: Oct. 2, 2011