Homo sapiens L. (human) [HSA]

FULL NAME: DNA topoisomerase 2-alpha


DESCRIPTION:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
Controls of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

DRUG TARGET

Podophyllotoxin: D05529
Etoposide: D00125 D04107
Teniposide: D02698
Doxorubicin: D01275 D03899
Daunorubicin: D01264 D07776
Epirubicin: D02214 D07901
Aclarubicin: D01911 D02756
Idarubicin: D01747 D08062
Mitoxantrone: D02166 D08224
Pirarubicin: D01885 D08386
Amrubicin: D07100 D08854
Amsacrine: D02321
Other: D01404 D03602 D04498 D04685 D04791 D06545

MISCELLANEOUS:
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the type II topoisomerase family.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


ENZYME REGULATION:
Specifically inhibited by the intercalating agent amsacrine.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylation has no effect on catalytic activity.


PROTEIN TYPE(S):
endonuclease
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MEVSPLQPVN ENMQVNKIKK NEDAKKRLSV ERIYQKKTQL EHILLRPDTY IGSVELVTQQ
        70         80         90        100        110        120
MWVYDEDVGI NYREVTFVPG LYKIFDEILV NAADNKQRDP KMSCIRVTID PENNLISIWN
       130        140        150        160        170        180
NGKGIPVVEH KVEKMYVPAL IFGQLLTSSN YDDDEKKVTG GRNGYGAKLC NIFSTKFTVE
       190        200        210        220        230        240
TASREYKKMF KQTWMDNMGR AGEMELKPFN GEDYTCITFQ PDLSKFKMQS LDKDIVALMV
       250        260        270        280        290        300
RRAYDIAGST KDVKVFLNGN KLPVKGFRSY VDMYLKDKLD ETGNSLKVIH EQVNHRWEVC
       310        320        330        340        350        360
LTMSEKGFQQ ISFVNSIATS KGGRHVDYVA DQIVTKLVDV VKKKNKGGVA VKAHQVKNHM
       370        380        390        400        410        420
WIFVNALIEN PTFDSQTKEN MTLQPKSFGS TCQLSEKFIK AAIGCGIVES ILNWVKFKAQ
       430        440        450        460        470        480
VQLNKKCSAV KHNRIKGIPK LDDANDAGGR NSTECTLILT EGDSAKTLAV SGLGVVGRDK
       490        500        510        520        530        540
YGVFPLRGKI LNVREASHKQ IMENAEINNI IKIVGLQYKK NYEDEDSLKT LRYGKIMIMT
       550        560        570        580        590        600
DQDQDGSHIK GLLINFIHHN WPSLLRHRFL EEFITPIVKV SKNKQEMAFY SLPEFEEWKS
       610        620        630        640        650        660
STPNHKKWKV KYYKGLGTST SKEAKEYFAD MKRHRIQFKY SGPEDDAAIS LAFSKKQIDD
       670        680        690        700        710        720
RKEWLTNFME DRRQRKLLGL PEDYLYGQTT TYLTYNDFIN KELILFSNSD NERSIPSMVD
       730        740        750        760        770        780
GLKPGQRKVL FTCFKRNDKR EVKVAQLAGS VAEMSSYHHG EMSLMMTIIN LAQNFVGSNN
       790        800        810        820        830        840
LNLLQPIGQF GTRLHGGKDS ASPRYIFTML SSLARLLFPP KDDHTLKFLY DDNQRVEPEW
       850        860        870        880        890        900
YIPIIPMVLI NGAEGIGTGW SCKIPNFDVR EIVNNIRRLM DGEEPLPMLP SYKNFKGTIE
       910        920        930        940        950        960
ELAPNQYVIS GEVAILNSTT IEISELPVRT WTQTYKEQVL EPMLNGTEKT PPLITDYREY
       970        980        990       1000       1010       1020
HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM VLFDHVGCLK KYDTVLDILR
      1030       1040       1050       1060       1070       1080
DFFELRLKYY GLRKEWLLGM LGAESAKLNN QARFILEKID GKIIIENKPK KELIKVLIQR
      1090       1100       1110       1120       1130       1140
GYDSDPVKAW KEAQQKVPDE EENEESDNEK ETEKSDSVTD SGPTFNYLLD MPLWYLTKEK
      1150       1160       1170       1180       1190       1200
KDELCRLRNE KEQELDTLKR KSPSDLWKED LATFIEELEA VEAKEKQDEQ VGLPGKGGKA
      1210       1220       1230       1240       1250       1260
KGKKTQMAEV LPSPRGQRVI PRITIEMKAE AEKKNKKKIK NENTEGSPQE DGVELEGLKQ
      1270       1280       1290       1300       1310       1320
RLEKKQKREP GTKTKKQTTL AFKPIKKGKK RNPWSDSESD RSSDESNFDV PPRETEPRRA
      1330       1340       1350       1360       1370       1380
ATKTKFTMDL DSDEDFSDFD EKTDDEDFVP SDASPPKTKT SPKLSNKELK PQKSVVSDLE
      1390       1400       1410       1420       1430       1440
ADDVKGSVPL SSSPPATHFP DETEITNPVP KKNVTVKKTA AKSQSSTSTT GAKKRAAPKG
      1450       1460       1470       1480       1490       1500
TKRDPALNSG VSQKPDPAKT KNRRKRKPST SDDSDSNFEK IVSKAVTSKK SKGESDDFHM
      1510       1520       1530
DFDSAVAPRA KSVRAKKPIK YLEESDEDDL F  

Encoded by TOP2A gene

FULL NAME: topoisomerase (DNA) II alpha 170kDa


OTHER NAME(S):
TOP2
TP2A


DESCRIPTION:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]


Nucleic acid sequence

        10         20         30         40         50         60
atggaagtgt caccattgca gcctgtaaat gaaaatatgc aagtcaacaa aataaagaaa
        70         80         90        100        110        120
aatgaagatg ctaagaaaag actgtctgtt gaaagaatct atcaaaagaa aacacaattg
       130        140        150        160        170        180
gaacatattt tgctccgccc agacacctac attggttctg tggaattagt gacccagcaa
       190        200        210        220        230        240
atgtgggttt acgatgaaga tgttggcatt aactataggg aagtcacttt tgttcctggt
       250        260        270        280        290        300
ttgtacaaaa tctttgatga gattctagtt aatgctgcgg acaacaaaca aagggaccca
       310        320        330        340        350        360
aaaatgtctt gtattagagt cacaattgat ccggaaaaca atttaattag tatatggaat
       370        380        390        400        410        420
aatggaaaag gtattcctgt tgttgaacac aaagttgaaa agatgtatgt cccagctctc
       430        440        450        460        470        480
atatttggac agctcctaac ttctagtaac tatgatgatg atgaaaagaa agtgacaggt
       490        500        510        520        530        540
ggtcgaaatg gctatggagc caaattgtgt aacatattca gtaccaaatt tactgtggaa
       550        560        570        580        590        600
acagccagta gagaatacaa gaaaatgttc aaacagacat ggatggataa tatgggaaga
       610        620        630        640        650        660
gctggtgaga tggaactcaa gcccttcaat ggagaagatt atacatgtat cacctttcag
       670        680        690        700        710        720
cctgatttgt ctaagtttaa aatgcaaagc ctggacaaag atattgttgc actaatggtc
       730        740        750        760        770        780
agaagagcat atgatattgc tggatccacc aaagatgtca aagtctttct taatggaaat
       790        800        810        820        830        840
aaactgccag taaaaggatt tcgtagttat gtggacatgt atttgaagga caagttggat
       850        860        870        880        890        900
gaaactggta actccttgaa agtaatacat gaacaagtaa accacaggtg ggaagtgtgt
       910        920        930        940        950        960
ttaactatga gtgaaaaagg ctttcagcaa attagctttg tcaacagcat tgctacatcc
       970        980        990       1000       1010       1020
aagggtggca gacatgttga ttatgtagct gatcagattg tgactaaact tgttgatgtt
      1030       1040       1050       1060       1070       1080
gtgaagaaga agaacaaggg tggtgttgca gtaaaagcac atcaggtgaa aaatcacatg
      1090       1100       1110       1120       1130       1140
tggatttttg taaatgcctt aattgaaaac ccaacctttg actctcagac aaaagaaaac
      1150       1160       1170       1180       1190       1200
atgactttac aacccaagag ctttggatca acatgccaat tgagtgaaaa atttatcaaa
      1210       1220       1230       1240       1250       1260
gctgccattg gctgtggtat tgtagaaagc atactaaact gggtgaagtt taaggcccaa
      1270       1280       1290       1300       1310       1320
gtccagttaa acaagaagtg ttcagctgta aaacataata gaatcaaggg aattcccaaa
      1330       1340       1350       1360       1370       1380
ctcgatgatg ccaatgatgc agggggccga aactccactg agtgtacgct tatcctgact
      1390       1400       1410       1420       1430       1440
gagggagatt cagccaaaac tttggctgtt tcaggccttg gtgtggttgg gagagacaaa
      1450       1460       1470       1480       1490       1500
tatggggttt tccctcttag aggaaaaata ctcaatgttc gagaagcttc tcataagcag
      1510       1520       1530       1540       1550       1560
atcatggaaa atgctgagat taacaatatc atcaagattg tgggtcttca gtacaagaaa
      1570       1580       1590       1600       1610       1620
aactatgaag atgaagattc attgaagacg cttcgttatg ggaagataat gattatgaca
      1630       1640       1650       1660       1670       1680
gatcaggacc aagatggttc ccacatcaaa ggcttgctga ttaattttat ccatcacaac
      1690       1700       1710       1720       1730       1740
tggccctctc ttctgcgaca tcgttttctg gaggaattta tcactcccat tgtaaaggta
      1750       1760       1770       1780       1790       1800
tctaaaaaca agcaagaaat ggcattttac agccttcctg aatttgaaga gtggaagagt
      1810       1820       1830       1840       1850       1860
tctactccaa atcataaaaa atggaaagtc aaatattaca aaggtttggg caccagcaca
      1870       1880       1890       1900       1910       1920
tcaaaggaag ctaaagaata ctttgcagat atgaaaagac atcgtatcca gttcaaatat
      1930       1940       1950       1960       1970       1980
tctggtcctg aagatgatgc tgctatcagc ctggccttta gcaaaaaaca gatagatgat
      1990       2000       2010       2020       2030       2040
cgaaaggaat ggttaactaa tttcatggag gatagaagac aacgaaagtt acttgggctt
      2050       2060       2070       2080       2090       2100
cctgaggatt acttgtatgg acaaactacc acatatctga catataatga cttcatcaac
      2110       2120       2130       2140       2150       2160
aaggaactta tcttgttctc aaattctgat aacgagagat ctatcccttc tatggtggat
      2170       2180       2190       2200       2210       2220
ggtttgaaac caggtcagag aaaggttttg tttacttgct tcaaacggaa tgacaagcga
      2230       2240       2250       2260       2270       2280
gaagtaaagg ttgcccaatt agctggatca gtggctgaaa tgtcttctta tcatcatggt
      2290       2300       2310       2320       2330       2340
gagatgtcac taatgatgac cattatcaat ttggctcaga attttgtggg tagcaataat
      2350       2360       2370       2380       2390       2400
ctaaacctct tgcagcccat tggtcagttt ggtaccaggc tacatggtgg caaggattct
      2410       2420       2430       2440       2450       2460
gctagtccac gatacatctt tacaatgctc agctctttgg ctcgattgtt atttccacca
      2470       2480       2490       2500       2510       2520
aaagatgatc acacgttgaa gtttttatat gatgacaacc agcgtgttga gcctgaatgg
      2530       2540       2550       2560       2570       2580
tacattccta ttattcccat ggtgctgata aatggtgctg aaggaatcgg tactgggtgg
      2590       2600       2610       2620       2630       2640
tcctgcaaaa tccccaactt tgatgtgcgt gaaattgtaa ataacatcag gcgtttgatg
      2650       2660       2670       2680       2690       2700
gatggagaag aacctttgcc aatgcttcca agttacaaga acttcaaggg tactattgaa
      2710       2720       2730       2740       2750       2760
gaactggctc caaatcaata tgtgattagt ggtgaagtag ctattcttaa ttctacaacc
      2770       2780       2790       2800       2810       2820
attgaaatct cagagcttcc cgtcagaaca tggacccaga catacaaaga acaagttcta
      2830       2840       2850       2860       2870       2880
gaacccatgt tgaatggcac cgagaagaca cctcctctca taacagacta tagggaatac
      2890       2900       2910       2920       2930       2940
catacagata ccactgtgaa atttgttgtg aagatgactg aagaaaaact ggcagaggca
      2950       2960       2970       2980       2990       3000
gagagagttg gactacacaa agtcttcaaa ctccaaacta gtctcacatg caactctatg
      3010       3020       3030       3040       3050       3060
gtgctttttg accacgtagg ctgtttaaag aaatatgaca cggtgttgga tattctaaga
      3070       3080       3090       3100       3110       3120
gacttttttg aactcagact taaatattat ggattaagaa aagaatggct cctaggaatg
      3130       3140       3150       3160       3170       3180
cttggtgctg aatctgctaa actgaataat caggctcgct ttatcttaga gaaaatagat
      3190       3200       3210       3220       3230       3240
ggcaaaataa tcattgaaaa taagcctaag aaagaattaa ttaaagttct gattcagagg
      3250       3260       3270       3280       3290       3300
ggatatgatt cggatcctgt gaaggcctgg aaagaagccc agcaaaaggt tccagatgaa
      3310       3320       3330       3340       3350       3360
gaagaaaatg aagagagtga caacgaaaag gaaactgaaa agagtgactc cgtaacagat
      3370       3380       3390       3400       3410       3420
tctggaccaa ccttcaacta tcttcttgat atgccccttt ggtatttaac caaggaaaag
      3430       3440       3450       3460       3470       3480
aaagatgaac tctgcaggct aagaaatgaa aaagaacaag agctggacac attaaaaaga
      3490       3500       3510       3520       3530       3540
aagagtccat cagatttgtg gaaagaagac ttggctacat ttattgaaga attggaggct
      3550       3560       3570       3580       3590       3600
gttgaagcca aggaaaaaca agatgaacaa gtcggacttc ctgggaaagg ggggaaggcc
      3610       3620       3630       3640       3650       3660
aaggggaaaa aaacacaaat ggctgaagtt ttgccttctc cgcgtggtca aagagtcatt
      3670       3680       3690       3700       3710       3720
ccacgaataa ccatagaaat gaaagcagag gcagaaaaga aaaataaaaa gaaaattaag
      3730       3740       3750       3760       3770       3780
aatgaaaata ctgaaggaag ccctcaagaa gatggtgtgg aactagaagg cctaaaacaa
      3790       3800       3810       3820       3830       3840
agattagaaa agaaacagaa aagagaacca ggtacaaaga caaagaaaca aactacattg
      3850       3860       3870       3880       3890       3900
gcatttaagc caatcaaaaa aggaaagaag agaaatccct ggtctgattc agaatcagat
      3910       3920       3930       3940       3950       3960
aggagcagtg acgaaagtaa ttttgatgtc cctccacgag aaacagagcc acggagagca
      3970       3980       3990       4000       4010       4020
gcaacaaaaa caaaattcac aatggatttg gattcagatg aagatttctc agattttgat
      4030       4040       4050       4060       4070       4080
gaaaaaactg atgatgaaga ttttgtccca tcagatgcta gtccacctaa gaccaaaact
      4090       4100       4110       4120       4130       4140
tccccaaaac ttagtaacaa agaactgaaa ccacagaaaa gtgtcgtgtc agaccttgaa
      4150       4160       4170       4180       4190       4200
gctgatgatg ttaagggcag tgtaccactg tcttcaagcc ctcctgctac acatttccca
      4210       4220       4230       4240       4250       4260
gatgaaactg aaattacaaa cccagttcct aaaaagaatg tgacagtgaa gaagacagca
      4270       4280       4290       4300       4310       4320
gcaaaaagtc agtcttccac ctccactacc ggtgccaaaa aaagggctgc cccaaaagga
      4330       4340       4350       4360       4370       4380
actaaaaggg atccagcttt gaattctggt gtctctcaaa agcctgatcc tgccaaaacc
      4390       4400       4410       4420       4430       4440
aagaatcgcc gcaaaaggaa gccatccact tctgatgatt ctgactctaa ttttgagaaa
      4450       4460       4470       4480       4490       4500
attgtttcga aagcagtcac aagcaagaaa tccaaggggg agagtgatga cttccatatg
      4510       4520       4530       4540       4550       4560
gactttgact cagctgtggc tcctcgggca aaatctgtac gggcaaagaa acctataaag
      4570       4580       4590
tacctggaag agtcagatga agatgatctg ttttaa  

Last modification date: Oct. 2, 2011