Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Minichromosome maintenance protein 5


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.
Present with 10300 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSFDRPEIYS APVLQGESPN DDDNTEIIKS FKNFILEFRL DSQFIYRDQL RNNILVKNYS
        70         80         90        100        110        120
LTVNMEHLIG YNEDIYKKLS DEPSDIIPLF ETAITQVAKR ISILSRAQSA NNNDKDPENT
       130        140        150        160        170        180
SMDTDSLLLN SLPTFQLILN SNANQIPLRD LDSEHVSKIV RLSGIIISTS VLSSRATYLS
       190        200        210        220        230        240
IMCRNCRHTT SITINNFNSI TGNTVSLPRS CLSTIESESS MANESNIGDE STKKNCGPDP
       250        260        270        280        290        300
YIIIHESSKF IDQQFLKLQE IPELVPVGEM PRNLTMTCDR YLTNKVIPGT RVTIVGIYSI
       310        320        330        340        350        360
YNSKNGAGSG RSGGGNGGSG VAIRTPYIKI LGIQSDVETS SIWNSVTMFT EEEEEEFLQL
       370        380        390        400        410        420
SRNPKLYEIL TNSIAPSIFG NEDIKKAIVC LLMGGSKKIL PDGMRLRGDI NVLLLGDPGT
       430        440        450        460        470        480
AKSQLLKFVE KVSPIAVYTS GKGSSAAGLT ASVQRDPMTR EFYLEGGAMV LADGGVVCID
       490        500        510        520        530        540
EFDKMRDEDR VAIHEAMEQQ TISIAKAGIT TVLNSRTSVL AAANPIYGRY DDLKSPGDNI
       550        560        570        580        590        600
DFQTTILSRF DMIFIVKDDH NEERDISIAN HVINIHTGNA NAMQNQQEEN GSEISIEKMK
       610        620        630        640        650        660
RYITYCRLKC APRLSPQAAE KLSSNFVTIR KQLLINELES TERSSIPITI RQLEAIIRIT
       670        680        690        700        710        720
ESLAKLELSP IAQERHVDEA IRLFQASTMD AASQDPIGGL NQASGTSLSE IRRFEQELKR
       730        740        750        760        770
RLPIGWSTSY QTLRREFVDT HRFSQLALDK ALYALEKHET IQLRHQGQNI YRSGV

Encoded by MCM5 gene


OTHER NAME(S):
BOB1
CDC46


Nucleic acid sequence

        10         20         30         40         50         60
atgtcatttg atagaccgga aatatacagt gctcctgttt tacaaggaga atctcctaac
        70         80         90        100        110        120
gacgatgata atactgaaat cataaagtcc tttaagaatt tcattttgga gttcagactt
       130        140        150        160        170        180
gactcgcaat ttatttacag agatcagtta aggaacaaca tccttgtgaa gaattattct
       190        200        210        220        230        240
ttaacggtta acatggagca tttgatcgga tataacgaag acatatataa gaaactatca
       250        260        270        280        290        300
gacgaacctt cagatatcat tccattattc gaaaccgcga tcacacaagt ggctaaaagg
       310        320        330        340        350        360
ataagtattc taagcagagc tcaatctgct aataacaatg acaaagatcc agaaaatact
       370        380        390        400        410        420
agtatggata ctgattctct cttattgaac tctttaccaa catttcaatt aattttaaac
       430        440        450        460        470        480
tccaatgcaa atcagattcc attgagagat ttggattctg aacacgtctc caagattgtc
       490        500        510        520        530        540
cgtttatcag gtattataat atccacgtca gttttatctt cccgtgccac gtacctttct
       550        560        570        580        590        600
ataatgtgca gaaattgcag acacacaaca tcaataacaa tcaacaattt caattctatc
       610        620        630        640        650        660
acaggcaata ccgtcagttt accacgttct tgcttatcta cgattgagag tgaatcttct
       670        680        690        700        710        720
atggcaaacg agtcgaatat tggtgatgaa tcgaccaaga aaaattgtgg acctgatcca
       730        740        750        760        770        780
tatattatta tccatgagtc ttcaaagttt attgatcaac agtttttaaa attacaggaa
       790        800        810        820        830        840
atcccagaac tggttccagt aggtgagatg cctagaaact taacaatgac ttgtgaccga
       850        860        870        880        890        900
tacctaacaa acaaagttat tcctggtacg agagtcacta tagtaggtat ttattccatc
       910        920        930        940        950        960
tataattcta aaaatggtgc cggatccgga aggagcgggg gtggaaatgg aggaagtggt
       970        980        990       1000       1010       1020
gttgctatta gaacacctta tatcaaaata ttaggtattc agtccgatgt agaaacctcc
      1030       1040       1050       1060       1070       1080
tctatttgga attcagtaac tatgtttact gaggaggaag aagaggaatt tctacagcta
      1090       1100       1110       1120       1130       1140
agtagaaacc cgaagcttta tgaaattttg accaactcta ttgccccctc tatttttggt
      1150       1160       1170       1180       1190       1200
aatgaagata taaaaaaagc cattgtatgt ttattgatgg gtggttccaa gaagatatta
      1210       1220       1230       1240       1250       1260
cccgatggta tgaggttaag aggtgatatc aatgtactat tattaggtga tccaggtacc
      1270       1280       1290       1300       1310       1320
gccaaatctc aactattgaa atttgtggag aaagtgtcac ctattgcggt atatacatct
      1330       1340       1350       1360       1370       1380
ggtaagggat cttctgcagc tgggttaact gccagtgtac aaagagatcc gatgacaaga
      1390       1400       1410       1420       1430       1440
gaattttatt tggaaggtgg tgctatggtg cttgccgatg gtggtgttgt atgcatcgat
      1450       1460       1470       1480       1490       1500
gaattcgata aaatgagaga tgaagataga gtggccattc atgaagctat ggagcagcaa
      1510       1520       1530       1540       1550       1560
acaatctcca tcgcaaaagc tggtatcact acagtgctaa attctagaac tagtgtttta
      1570       1580       1590       1600       1610       1620
gcggctgcta atccgatata cggccggtat gatgatttga agtctcctgg tgacaacatt
      1630       1640       1650       1660       1670       1680
gatttccaaa ctactatttt atcccgtttt gatatgattt ttattgttaa ggatgaccat
      1690       1700       1710       1720       1730       1740
aatgaagaac gtgatatttc aatagctaac cacgttatta atattcatac aggaaatgct
      1750       1760       1770       1780       1790       1800
aatgctatgc aaaaccaaca agaggaaaat ggcagtgaaa ttagtattga aaagatgaaa
      1810       1820       1830       1840       1850       1860
cgttacatta cgtattgtag attgaaatgt gcaccaagac tttcaccgca ggccgctgaa
      1870       1880       1890       1900       1910       1920
aaactgtcat cgaacttcgt caccattagg aagcaattat taatcaacga attagagtca
      1930       1940       1950       1960       1970       1980
acggaaaggt cgtctattcc aattaccatt cgtcaattag aagctattat tagaataaca
      1990       2000       2010       2020       2030       2040
gaatcattag ccaagttaga attaagtcct attgcacagg agagacatgt tgacgaagct
      2050       2060       2070       2080       2090       2100
attagattgt ttcaagcttc cacaatggac gcagcgtctc aggatccaat tggcggctta
      2110       2120       2130       2140       2150       2160
aatcaagcaa gcggaacatc tttgtcagaa atccgtcgtt ttgaacaaga actaaaaaga
      2170       2180       2190       2200       2210       2220
agattaccta taggctggtc tacttcttat caaactttga ggagagaatt tgtagataca
      2230       2240       2250       2260       2270       2280
catagatttt ctcaattagc actggataag gccttatatg ccctagagaa gcatgaaaca
      2290       2300       2310       2320
attcaattga gacaccaggg acagaatatt tacagaagtg gtgtatga 

Last modification date: Oct. 2, 2011