Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA-cytosine methyltransferase


DESCRIPTION:
This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

STRUCTURE SIMILARITY:
Belongs to the C5-methyltransferase family.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.


PROTEIN TYPE(S):
DNA methyltransferase


RELATED PATHWAY(S):
DNA replication
heterochromatin formation


Amino acids sequence

        10         20         30         40         50         60
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL KRALANDSAW
        70         80         90        100        110        120
HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR RGFESIGGQC VFTSEWNKHA
       130        140        150        160        170        180
VRTYKANHYC DPATHHFNED IRDITLSHKE GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF
       190        200        210        220        230        240
SLAGVSKKNS LGRAHGFACD TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI
       250        260        270        280        290        300
IMQTLDELGY DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS
       310        320        330        340        350        360
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM VYPNNPQSVT
       370        380        390        400        410        420
RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR RLTPRECARL MGFEAPGEAK
       430        440        450        460        470
FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL EPKIKQAVAL RQQEAQHGRR SR

Encoded by dcm gene


OTHER NAME(S):
ECK1959
JW1944
mec


DESCRIPTION:
Dcm is a DNA cytosine methyltransferase that preferentially methylates sites of sequence 5' C-MeC-T 3' . [More information is available at EcoCyc: EG10211].


Nucleic acid sequence

        10         20         30         40         50         60
atgcaggaaa atatatcagt aaccgattca tacagcaccg ggaatgccgc acaggcaatg
        70         80         90        100        110        120
ctggagaaac tgctgcaaat ttatgatgtt aaaacgttgg tggcgcagct taatggtgta
       130        140        150        160        170        180
ggtgagaatc actggagcgc ggcaatttta aaacgtgcgc tggcgaatga ctcggcatgg
       190        200        210        220        230        240
caccgtttaa gtgagaaaga gttcgcccat ctgcaaacgt tattacccaa accaccggca
       250        260        270        280        290        300
catcatccgc attatgcgtt tcgctttatc gatctattcg ccggaattgg cggcatccgt
       310        320        330        340        350        360
cgcggttttg aatcgattgg cggacagtgc gtgtttacca gcgaatggaa caaacatgcg
       370        380        390        400        410        420
gtacgcactt ataaagccaa ccattattgc gatccggcga cgcatcattt taatgaagat
       430        440        450        460        470        480
atccgcgaca tcaccctcag ccataaagaa ggcgtgagtg atgaggcggc ggcggaacat
       490        500        510        520        530        540
attcgtcaac acattcctga acacgatgtt ttactggccg gtttcccttg tcagccattt
       550        560        570        580        590        600
tcgctggctg gcgtatcgaa aaagaactcg ctcgggcggg cgcacggttt tgcctgcgat
       610        620        630        640        650        660
acccagggca cgctgttttt tgatgtggta cgcattatcg acgcgcgtcg tccggcgatg
       670        680        690        700        710        720
tttgtgctcg aaaacgtcaa aaacctgaaa agtcacgacc agggtaaaac gttccgcatc
       730        740        750        760        770        780
atcatgcaga cgctggacga actgggctat gacgtggctg atgcagaaga taatgggcca
       790        800        810        820        830        840
gacgatccga aaatcatcga cggcaaacat tttctgccgc agcaccgtga acgcatcgtg
       850        860        870        880        890        900
ctggtgggtt ttcgtcgcga tctgaatctg aaagccgatt ttaccctgcg tgatatcagc
       910        920        930        940        950        960
gaatgtttcc ctgcgcagcg agtgacgctg gcgcagctgt tggacccgat ggtcgaggcg
       970        980        990       1000       1010       1020
aaatatatcc tgacgccggt gctgtggaag tacctctatc gatatgcgaa aaaacatcag
      1030       1040       1050       1060       1070       1080
gcgcgcggta acggcttcgg ttatggaatg gtttatccga acaatccgca aagcgtcacg
      1090       1100       1110       1120       1130       1140
cgtacgctgt ctgcgcgtta ttacaaagat ggcgcggaaa ttttaatcga tcgcggctgg
      1150       1160       1170       1180       1190       1200
gatatggcca cgggtgagaa agactttgac gatccgctga atcagcaaca tcgtccacgt
      1210       1220       1230       1240       1250       1260
cggttaacgc ctcgggaatg cgcgcgctta atgggttttg aagcgccggg agaagcgaaa
      1270       1280       1290       1300       1310       1320
ttccgtattc cggtttcgga cactcaggcc tatcgccagt tcggtaactc ggtggtcgtg
      1330       1340       1350       1360       1370       1380
ccggtctttg ccgcggtggc aaaactgctt gagccaaaaa tcaaacaggc ggtggcgttg
      1390       1400       1410
cgtcagcaag aggcacaaca tggccgacgt tcacgataa  

Last modification date: Oct. 2, 2011