Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA replication licensing factor MCM4


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Required for S phase execution.

MISCELLANEOUS:
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.
Present with 8800 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSQQSSSPTK EDNNSSSPVV PNPDSVPPQL SSPALFYSSS SSQGDIYGRN NSQNLSQGEG
        70         80         90        100        110        120
NIRAAIGSSP LNFPSSSQRQ NSDVFQSQGR QGRIRSSASA SGRSRYHSDL RSDRALPTSS
       130        140        150        160        170        180
SSLGRNGQNR VHMRRNDIHT SDLSSPRRIV DFDTRSGVNT LDTSSSSAPP SEASEPLRII
       190        200        210        220        230        240
WGTNVSIQEC TTNFRNFLMS FKYKFRKILD EREEFINNTT DEELYYIKQL NEMRELGTSN
       250        260        270        280        290        300
LNLDARNLLA YKQTEDLYHQ LLNYPQEVIS IMDQTIKDCM VSLIVDNNLD YDLDEIETKF
       310        320        330        340        350        360
YKVRPYNVGS CKGMRELNPN DIDKLINLKG LVLRSTPVIP DMKVAFFKCN VCDHTMAVEI
       370        380        390        400        410        420
DRGVIQEPAR CERIDCNEPN SMSLIHNRCS FADKQVIKLQ ETPDFVPDGQ TPHSISLCVY
       430        440        450        460        470        480
DELVDSCRAG DRIEVTGTFR SIPIRANSRQ RVLKSLYKTY VDVVHVKKVS DKRLDVDTST
       490        500        510        520        530        540
IEQELMQNKV DHNEVEEVRQ ITDQDLAKIR EVAAREDLYS LLARSIAPSI YELEDVKKGI
       550        560        570        580        590        600
LLQLFGGTNK TFTKGGRYRG DINILLCGDP STSKSQILQY VHKITPRGVY TSGKGSSAVG
       610        620        630        640        650        660
LTAYITRDVD TKQLVLESGA LVLSDGGVCC IDEFDKMSDS TRSVLHEVME QQTISIAKAG
       670        680        690        700        710        720
IITTLNARSS ILASANPIGS RYNPNLPVTE NIDLPPPLLS RFDLVYLVLD KVDEKNDREL
       730        740        750        760        770        780
AKHLTNLYLE DKPEHISQDD VLPVEFLTMY ISYAKEHIHP IITEAAKTEL VRAYVGMRKM
       790        800        810        820        830        840
GDDSRSDEKR ITATTRQLES MIRLAEAHAK MKLKNVVELE DVQEAVRLIR SAIKDYATDP
       850        860        870        880        890        900
KTGKIDMNLV QTGKSVIQRK LQEDLSREIM NVLKDQASDS MSFNELIKQI NEHSQDRVES
       910        920        930
SDIQEALSRL QQEDKVIVLG EGVRRSVRLN NRV  

Encoded by MCM4 gene


OTHER NAME(S):
CDC54
HCD21


Nucleic acid sequence

        10         20         30         40         50         60
atgtctcaac agtctagctc tccaacaaaa gaggataata actccagctc ccccgttgtg
        70         80         90        100        110        120
cctaatcctg attctgttcc accacagctt tcttccccag ctctatttta tagctcctct
       130        140        150        160        170        180
tcatcacaag gtgatatcta tggtcgcaac aatagccaga acttaagtca gggagaggga
       190        200        210        220        230        240
aacatcagag ctgctatagg ttcttctcca ctaaattttc catcttcttc ccaaagacaa
       250        260        270        280        290        300
aattccgatg ttttccaatc tcaaggcaga cagggcagaa ttcgttcttc tgcgagcgct
       310        320        330        340        350        360
tctggaaggt ctagatatca ttctgatctg agaagtgata gagcactgcc tacttcttcc
       370        380        390        400        410        420
tcttctttag gccgtaatgg tcaaaaccgt gtacacatgc gaagaaatga tattcataca
       430        440        450        460        470        480
tctgatttat cctctccaag aagaattgtg gattttgata ctagatccgg cgtgaatact
       490        500        510        520        530        540
ttggatactt cttcttcttc tgctcctcca tcggaagcca gtgaaccctt gagaataatt
       550        560        570        580        590        600
tggggtacca acgtcagtat ccaggaatgt acaactaatt ttcgtaattt tttgatgtct
       610        620        630        640        650        660
ttcaagtata aattccgtaa aatattggat gaaagggagg aattcattaa caatactacc
       670        680        690        700        710        720
gacgaagaac tatactatat caagcagctt aatgaaatga gagaacttgg tacctctaat
       730        740        750        760        770        780
ttaaacttgg atgctagaaa cttacttgct tacaagcaaa cagaagactt ataccatcaa
       790        800        810        820        830        840
ctattaaatt atcctcaaga agtgatttct atcatggatc aaactatcaa ggactgtatg
       850        860        870        880        890        900
gtttccttaa tagtagacaa caatttggat tacgatttag atgaaataga gaccaagttt
       910        920        930        940        950        960
tataaagtaa ggccttacaa tgtaggttct tgtaaaggta tgcgcgaatt gaatccaaat
       970        980        990       1000       1010       1020
gatattgata aattgataaa cttaaagggt cttgtcctga gatcaactcc ggttatccct
      1030       1040       1050       1060       1070       1080
gatatgaagg tagcgttttt caaatgcaac gtctgcgatc atacaatggc agtggaaatt
      1090       1100       1110       1120       1130       1140
gatagaggag ttatacaaga gcccgctagg tgtgaacgta ttgattgtaa tgaacccaat
      1150       1160       1170       1180       1190       1200
tccatgtcac tgattcacaa caggtgttca ttcgcagata aacaagtcat taagttacag
      1210       1220       1230       1240       1250       1260
gaaactccag actttgtgcc tgatggacaa acgcctcact ctatctcatt atgtgtttac
      1270       1280       1290       1300       1310       1320
gatgaattag tggattcttg cagggcgggc gatcgtattg aggtgactgg cacgttcagg
      1330       1340       1350       1360       1370       1380
tccatcccca ttagagctaa ttccaggcaa cgcgtactaa agtcgttgta taaaacatac
      1390       1400       1410       1420       1430       1440
gtcgatgtgg tccacgttaa aaaagtttca gataagaggt tagacgtcga tacttctact
      1450       1460       1470       1480       1490       1500
attgaacaag aattaatgca gaacaaggta gatcataacg aggtcgaaga agtaagacaa
      1510       1520       1530       1540       1550       1560
attactgatc aggatttagc aaaaattcgc gaggttgcgg caagagaaga tttatacagt
      1570       1580       1590       1600       1610       1620
ttattagccc gctctattgc cccaagtatt tacgagctag aagatgtcaa gaagggtata
      1630       1640       1650       1660       1670       1680
ttacttcagc tatttggcgg cacgaataaa acctttacca agggtggccg ttatagaggt
      1690       1700       1710       1720       1730       1740
gacataaata ttttactttg tggggatcct tctacttcca aatcgcaaat tttgcaatac
      1750       1760       1770       1780       1790       1800
gttcacaaaa ttactcctcg tggtgtgtat acttcgggta aaggttcatc tgccgttggt
      1810       1820       1830       1840       1850       1860
ttaactgctt atattacaag ggatgtcgac acaaaacaac ttgttttgga aagtggtgca
      1870       1880       1890       1900       1910       1920
ttagtattgt ctgatggagg tgtttgttgt attgacgagt ttgataaaat gagtgattct
      1930       1940       1950       1960       1970       1980
acaaggtccg tcttgcacga agtcatggaa cagcagacta tttcaatcgc aaaagcggga
      1990       2000       2010       2020       2030       2040
attatcacaa cacttaatgc cagaagttct attttggcca gtgctaaccc aattggttca
      2050       2060       2070       2080       2090       2100
cgctacaacc ccaatttgcc tgtgactgaa aatattgatc taccgccccc actactttcg
      2110       2120       2130       2140       2150       2160
agattcgatc tggtctatct tgtccttgat aaggttgatg agaaaaatga cagagaacta
      2170       2180       2190       2200       2210       2220
gccaaacact taacaaatct ttacctggaa gataagcccg aacatatttc tcaagacgac
      2230       2240       2250       2260       2270       2280
gtgctaccag ttgaattttt aacgatgtat attagttatg caaaggagca catacaccca
      2290       2300       2310       2320       2330       2340
ataatcaccg aggccgctaa gactgagctt gttcgtgctt atgtaggaat gagaaagatg
      2350       2360       2370       2380       2390       2400
ggtgacgatt cgagatccga tgagaagaga atcacagcta ccacaagaca acttgaaagt
      2410       2420       2430       2440       2450       2460
atgattcgtt tggctgaggc gcacgccaaa atgaaattga aaaacgtcgt agagctggag
      2470       2480       2490       2500       2510       2520
gatgttcaag aagccgttag attaattaga tcagccataa aagattatgc aacagaccct
      2530       2540       2550       2560       2570       2580
aaaaccggta aaatcgacat gaatttagtt caaacaggta aatcagttat tcagagaaaa
      2590       2600       2610       2620       2630       2640
ctacaggagg atttgtcaag ggaaattatg aatgttttga aggatcaggc atcagactca
      2650       2660       2670       2680       2690       2700
atgtcattca atgagcttat aaaacaaatc aatgaacact ctcaagatag agttgagtct
      2710       2720       2730       2740       2750       2760
tctgatatcc aggaagcctt gtcaagattg caacaagagg acaaggtcat tgtccttggc
      2770       2780       2790       2800
gagggtgtaa ggagatcagt tcgcctgaat aaccgtgtct ga 

Last modification date: Oct. 2, 2011