Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: CPD photolyase


DESCRIPTION:
Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

MISCELLANEOUS:
Upon absorption of visible light electrons are transferred from Trp-307 through Trp-360 to Trp 383, and from there to FADH, giving rise to the fully reduced catalytic FADH(-).
There are only 10-20 molecules of photolyase per E.coli cell.


STRUCTURE SIMILARITY:
Contains 1 DNA photolyase domain.
Belongs to the DNA photolyase class-1 family.


CATALYTIC ACTIVITY:
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
CPD


Amino acids sequence

        10         20         30         40         50         60
MTTHLVWFRQ DLRLHDNLAL AAACRNSSAR VLALYIATPR QWATHNMSPR QAELINAQLN
        70         80         90        100        110        120
GLQIALAEKG IPLLFREVDD FVASVEIVKQ VCAENSVTHL FYNYQYEVNE RARDVEVERA
       130        140        150        160        170        180
LRNVVCEGFD DSVILPPGAV MTGNHEMYKV FTPFKNAWLK RLREGMPECV AAPKVRSSGS
       190        200        210        220        230        240
IEPSPSITLN YPRQSFDTAH FPVEEKAAIA QLRQFCQNGA GEYEQQRDFP AVEGTSRLSA
       250        260        270        280        290        300
SLATGGLSPR QCLHRLLAEQ PQALDGGAGS VWLNELIWRE FYRHLITYHP SLCKHRPFIA
       310        320        330        340        350        360
WTDRVQWQSN PAHLQAWQEG KTGYPIVDAA MRQLNSTGWM HNRLRMITAS FLVKDLLIDW
       370        380        390        400        410        420
REGERYFMSQ LIDGDLAANN GGWQWAASTG TDAAPYFRIF NPTTQGEKFD HEGEFIRQWL
       430        440        450        460        470
PELRDVPGKV VHEPWKWAQK AGVTLDYPQP IVEHKEARVQ TLAAYEAARK GK

Encoded by phr gene


OTHER NAME(S):
ECK0697
JW0698
phrB


DESCRIPTION:
The flavoprotein DNA photolyase uses light to repair cyclobutylpyrimidine dimers in DNA. [More information is available at EcoGene: EG10736]. Exposing Escherichia coli to UV radiation at wavelengths near the absorption maximum of DNA produces cyclobutane-type pyrimidine dimers (CPDs) as well as, to a lesser degree, 6-4 pyrimidine-pyrimidones in its DNA. [More information is available at EcoCyc: EG10736].


Nucleic acid sequence

        10         20         30         40         50         60
atgactaccc atctggtctg gtttcgccag gatttacgtc tgcacgataa tctcgcactg
        70         80         90        100        110        120
gctgccgcct gccgcaattc gtctgcacgc gtgctggcgt tgtatatcgc tacaccacgc
       130        140        150        160        170        180
cagtgggcga cgcataacat gtcgccgcgt caggctgaac ttatcaatgc tcaactgaat
       190        200        210        220        230        240
gggctacaaa tagcgcttgc ggaaaaaggt attcctttat tgttccgtga agtggatgac
       250        260        270        280        290        300
tttgtcgcca gtgtcgaaat agttaaacag gtgtgcgcgg aaaacagcgt tacccacctg
       310        320        330        340        350        360
ttttataact atcagtatga agtgaatgag cgggcgcggg atgtggaagt tgaaagagcg
       370        380        390        400        410        420
ctgcgtaacg tggtgtgtga aggatttgat gacagcgtga tcctgccgcc tggcgcggtg
       430        440        450        460        470        480
atgaccggta atcacgagat gtacaaagtc tttacgcctt ttaagaatgc ctggctgaaa
       490        500        510        520        530        540
cggctgcggg aagggatgcc ggagtgcgtc gctgcgccaa aagttcgtag tagcggatcg
       550        560        570        580        590        600
atagagccct cgccatccat tacgctgaat tatcctcgtc agtctttcga tactgcgcat
       610        620        630        640        650        660
tttccggtgg aagaaaaagc ggcgattgcg caattacgcc agttttgcca gaacggtgcc
       670        680        690        700        710        720
ggagaatatg agcaacaacg agattttccg gcagtggaag gcaccagccg tttgtcggcc
       730        740        750        760        770        780
agcctggcaa cgggcgggtt atcgcctcgc cagtgcttgc atcgcttgtt ggctgaacag
       790        800        810        820        830        840
ccgcaggcgc tggacggtgg ggccggtagt gtctggctta atgagctgat ctggcgcgag
       850        860        870        880        890        900
ttttaccgtc acctgataac gtatcacccc tcgttgtgta aacatcgtcc atttattgcc
       910        920        930        940        950        960
tggacggatc gtgtacagtg gcagagcaat cccgcacatt tacaggcctg gcaggaaggc
       970        980        990       1000       1010       1020
aaaacgggat acccgattgt tgatgccgct atgcgtcagc ttaacagcac tggctggatg
      1030       1040       1050       1060       1070       1080
cataacaggc tacggatgat tacagccagt tttctggtga aagatttatt gatcgactgg
      1090       1100       1110       1120       1130       1140
cgcgaaggcg agcgatattt catgtcgcag ctgattgatg gtgatttggc agccaataac
      1150       1160       1170       1180       1190       1200
ggtggctggc agtgggccgc ttcaaccgga accgatgcag cgccgtattt tcgtattttc
      1210       1220       1230       1240       1250       1260
aacccgacaa cccagggcga gaaatttgat catgagggcg agtttatccg ccagtggcta
      1270       1280       1290       1300       1310       1320
ccggaactgc gcgatgtgcc agggaaagtg gtgcatgagc cgtggaagtg ggcgcagaaa
      1330       1340       1350       1360       1370       1380
gcaggtgtga cgctggatta tccgcaaccg atagtcgagc acaaagaagc gagagtacaa
      1390       1400       1410
acgttggcag cgtatgaggc ggcgcggaag gggaaataa  

Last modification date: Dec. 20, 2011