Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Methylated-DNA--protein-cysteine methyltransferase


DESCRIPTION:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Can also repair O-4-methylthymine.

MISCELLANEOUS:
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.


STRUCTURE SIMILARITY:
Belongs to the MGMT family.


CATALYTIC ACTIVITY:
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
O6-methyl G (O6meG)
O4-methyl T (O4meT)


Amino acids sequence

        10         20         30         40         50         60
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG YERISATNPG
        70         80         90        100        110        120
GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI PCGQVMHYGQ LAEQLGRPGA
       130        140        150        160        170
ARAVGAANGS NPISIVVPCH RVIGRNGTMT GYAGGVQRKE WLLRHEGYLL L

Encoded by ogt gene


OTHER NAME(S):
ECK1331
JW1329


DESCRIPTION:
Ogt is the second (after Ada) methyltransferase enzyme for the direct repair of alkylating lesions in Escherichia coli and is responsible the background constitutive alkyl-transfer when the Ada response is not induced. [More information is available at EcoCyc: EG10668].


Nucleic acid sequence

        10         20         30         40         50         60
atgctgagat tacttgaaga aaaaattgcc acgccactgg gtccactgtg ggtgatttgc
        70         80         90        100        110        120
gatgagcaat ttcgcctgcg ggcggttgaa tgggaagagt acagcgaacg catggtgcag
       130        140        150        160        170        180
ctgctggaca tccattatcg caaagaaggc tatgagcgca tttctgccac caatccaggc
       190        200        210        220        230        240
ggtttaagcg acaagcttcg tgaatatttt gccggtaatc ttagcattat tgatacgctt
       250        260        270        280        290        300
cccactgcta cgggggggac gccatttcag cgcgaagtct ggaaaacact acgcactatc
       310        320        330        340        350        360
ccctgcgggc aggtaatgca ttacggccaa ctggctgagc aattgggccg tcctggcgcg
       370        380        390        400        410        420
gcgcgtgccg ttggtgcggc aaacggatcg aatcccatca gcatcgtcgt accttgccat
       430        440        450        460        470        480
cgggttattg gccgaaacgg caccatgacc ggatatgcag gcggagttca gcgaaaagag
       490        500        510
tggttattgc gccatgaagg ttatcttttg ctgtaa  

Last modification date: Oct. 2, 2011