Homo sapiens L. (human) [HSA]

FULL NAME: Tyrosyl-DNA phosphodiesterase 2


DESCRIPTION:
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

STRUCTURE SIMILARITY:
Belongs to the CCR4/nocturin family.


PROTEIN TYPE(S):
DNase
5'-3' exonuclease


RELATED DAMAGE:
5'-TOP2 termini


Amino acids sequence

        10         20         30         40         50         60
MELGSCLEGG REAAEEEGEP EVKKRRLLCV EFASVASCDA AVAQCFLAEN DWEMERALNS
        70         80         90        100        110        120
YFEPPVEESA LERRPETISE PKTYVDLTNE ETTDSTTSKI SPSEDTQQEN GSMFSLITWN
       130        140        150        160        170        180
IDGLDLNNLS ERARGVCSYL ALYSPDVIFL QEVIPPYYSY LKKRSSNYEI ITGHEEGYFT
       190        200        210        220        230        240
AIMLKKSRVK LKSQEIIPFP STKMMRNLLC VHVNVSGNEL CLMTSHLEST RGHAAERMNQ
       250        260        270        280        290        300
LKMVLKKMQE APESATVIFA GDTNLRDREV TRCGGLPNNI VDVWEFLGKP KHCQYTWDTQ
       310        320        330        340        350        360
MNSNLGITAA CKLRFDRIFF RAAAEEGHII PRSLDLLGLE KLDCGRFPSD HWGLLCNLDI

IL     

Encoded by TDP2 gene

FULL NAME: tyrosyl-DNA phosphodiesterase 2


OTHER NAME(S):
EAP2
AD022
TTRAP
MGC9099
dJ30M3.3
MGC111021
RP1-30M3.3


DESCRIPTION:
This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggagttgg ggagttgcct ggagggcggg agggaggcgg cggaggaaga gggcgagcct
        70         80         90        100        110        120
gaggtgaaaa agcggcgact tctgtgtgtg gagtttgcct cggtcgcaag ctgcgatgcc
       130        140        150        160        170        180
gcagtggctc agtgcttcct ggccgagaac gactgggaga tggaaagggc tctgaactcc
       190        200        210        220        230        240
tacttcgagc ctccggtgga ggagagcgcc ttggaacgcc gacctgaaac catctctgag
       250        260        270        280        290        300
cccaagacct atgttgacct aaccaatgaa gaaacaactg attccaccac ttctaaaatc
       310        320        330        340        350        360
agcccatctg aagatactca gcaagaaaat ggcagcatgt tctctctcat tacctggaat
       370        380        390        400        410        420
attgatggat tagatctaaa caatctgtca gagagggctc gaggggtgtg ttcctactta
       430        440        450        460        470        480
gctttgtaca gcccagatgt gatatttcta caggaagtta ttcccccata ttatagctac
       490        500        510        520        530        540
ctaaagaaga gatcaagtaa ttatgagatt attacaggtc atgaagaagg atatttcaca
       550        560        570        580        590        600
gctataatgt tgaagaaatc aagagtgaaa ttaaaaagcc aagagattat tccttttcca
       610        620        630        640        650        660
agtaccaaaa tgatgagaaa ccttttatgt gtgcatgtga acgtgtcagg aaatgagctt
       670        680        690        700        710        720
tgccttatga catcccattt ggagagcacc agagggcatg ctgcggaacg aatgaatcag
       730        740        750        760        770        780
ttaaaaatgg ttttaaagaa aatgcaagag gctccagagt cagctacagt tatatttgca
       790        800        810        820        830        840
ggagatacaa atctaaggga tcgagaggtt accagatgtg gtggtttacc caacaacatt
       850        860        870        880        890        900
gtggatgtct gggagttttt gggcaaacct aaacattgcc agtatacatg ggatacacaa
       910        920        930        940        950        960
atgaactcta atcttggaat aactgctgct tgtaaacttc gttttgatcg aatatttttc
       970        980        990       1000       1010       1020
agagcagcag cagaagaggg acacattatt ccccgaagtt tggaccttct tggattagaa
      1030       1040       1050       1060       1070       1080
aaactggact gtggtagatt tcctagtgat cactggggtc ttctgtgcaa cttagatata

atattgtaa     

Last modification date: Oct. 2, 2011