Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA replication licensing factor MCM3


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.

MISCELLANEOUS:
Present with 35100 molecules/cell in log phase SD medium.
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MEGSTGFDGD ATTFFAPDAV FGDRVRRFQE FLDTFTSYRD SVRSIQVYNS NNAANYNDDQ
        70         80         90        100        110        120
DDADERDLLG DDDGDDLEKE KKAASSTSLN ILPHRIIISL DDLREFDRSF WSGILVEPAY
       130        140        150        160        170        180
FIPPAEKALT DLADSMDDVP HPNASAVSSR HPWKLSFKGS FGAHALSPRT LTAQHLNKLV
       190        200        210        220        230        240
SVEGIVTKTS LVRPKLIRSV HYAAKTGRFH YRDYTDATTT LTTRIPTPAI YPTEDTEGNK
       250        260        270        280        290        300
LTTEYGYSTF IDHQRITVQE MPEMAPAGQL PRSIDVILDD DLVDKTKPGD RVNVVGVFKS
       310        320        330        340        350        360
LGAGGMNQSN SNTLIGFKTL ILGNTVYPLH ARSTGVAARQ MLTDFDIRNI NKLSKKKDIF
       370        380        390        400        410        420
DILSQSLAPS IYGHDHIKKA ILLMLMGGVE KNLENGSHLR GDINILMVGD PSTAKSQLLR
       430        440        450        460        470        480
FVLNTASLAI ATTGRGSSGV GLTAAVTTDR ETGERRLEAG AMVLADRGVV CIDEFDKMTD
       490        500        510        520        530        540
VDRVAIHEVM EQQTVTIAKA GIHTTLNARC SVIAAANPVF GQYDVNRDPH QNIALPDSLL
       550        560        570        580        590        600
SRFDLLFVVT DDINEIRDRS ISEHVLRTHR YLPPGYLEGE PVRERLNLSL AVGEDADINP
       610        620        630        640        650        660
EEHSNSGAGV ENEGEDDEDH VFEKFNPLLQ AGAKLAKNKG NYNGTEIPKL VTIPFLRKYV
       670        680        690        700        710        720
QYAKERVIPQ LTQEAINVIV KNYTDLRNDD NTKKSPITAR TLETLIRLAT AHAKVRLSKT
       730        740        750        760        770        780
VNKVDAKVAA NLLRFALLGE DIGNDIDEEE SEYEEALSKR SPQKSPKKRQ RVRQPASNSG
       790        800        810        820        830        840
SPIKSTPRRS TASSVNATPS SARRILRFQD DEQNAGEDDN DIMSPLPADE EAELQRRLQL
       850        860        870        880        890        900
GLRVSPRRRE HLHAPEEGSS GPLTEVGTPR LPNVSSAGQD DEQQQSVISF DNVEPGTIST
       910        920        930        940        950        960
GRLSLISGII ARLMQTEIFE EESYPVASLF ERINEELPEE EKFSAQEYLA GLKIMSDRNN
       970
LMVADDKVWR V    

Encoded by MCM3 gene


Nucleic acid sequence

        10         20         30         40         50         60
atggaaggct caacgggatt tgatggagac gctactactt ttttcgctcc agacgctgtg
        70         80         90        100        110        120
tttggtgaca gagtgcgcag atttcaagag tttttagata ctttcacctc atacagagac
       130        140        150        160        170        180
tctgtaaggt ccatacaagt ttacaacagc aataacgcgg ccaactacaa cgatgatcaa
       190        200        210        220        230        240
gatgacgcag acgaacgaga tttgctaggt gatgacgacg gtgatgatct tgaaaaggaa
       250        260        270        280        290        300
aagaaagcag catcgtccac ctcattgaat atactccctc acaggattat catctcgctt
       310        320        330        340        350        360
gatgacttga gagaattcga caggtcgttc tggtcgggca ttttagtcga accagcatac
       370        380        390        400        410        420
ttcatcccgc ctgccgaaaa ggcgcttact gacctagcag attccatgga cgatgttcca
       430        440        450        460        470        480
catcccaatg cctctgcagt atcgtctcgc catccttgga agctttcgtt caaaggctca
       490        500        510        520        530        540
tttggtgcac acgcattgtc tcctcgtact ctaacggcac aacatttaaa caaactggtc
       550        560        570        580        590        600
tctgttgagg gtatcgtaac taagacttcg ttggtcaggc caaagcttat cagatctgtc
       610        620        630        640        650        660
cactacgcgg caaagactgg tagattccat tacagagatt atacagatgc tactacaact
       670        680        690        700        710        720
ctcaccaccc gcatcccaac gcctgccatc tatccaacgg aggacactga aggtaacaaa
       730        740        750        760        770        780
ctaaccaccg aatatgggta tagtacgttc atagaccatc agcgtatcac tgtgcaagaa
       790        800        810        820        830        840
atgcccgaaa tggcccccgc tggccaactt cccaggtcca ttgacgtcat tctcgatgac
       850        860        870        880        890        900
gaccttgtgg acaagaccaa gccaggtgac agagttaacg ttgtcggggt attcaagtcg
       910        920        930        940        950        960
cttggtgctg gtggcatgaa ccagtccaac tctaatacat tgatcgggtt caaaactctg
       970        980        990       1000       1010       1020
atcctaggta atacggtgta tcctctccac gccagatcca cgggtgtcgc tgcgagacaa
      1030       1040       1050       1060       1070       1080
atgttgacag atttcgatat aagaaatatc aataaactat ccaaaaaaaa ggacattttc
      1090       1100       1110       1120       1130       1140
gatatcttgt ctcaatcttt agcgccttct atttatggac atgaccatat aaagaaagcc
      1150       1160       1170       1180       1190       1200
attttattga tgctcatggg aggtgtggag aaaaatttag aaaatggctc gcatttaaga
      1210       1220       1230       1240       1250       1260
ggtgacatca atatcctaat ggtgggtgat ccatccactg ccaagtccca attgctaagg
      1270       1280       1290       1300       1310       1320
tttgtgttga atacagcatc actggcaatt gctactactg gtagaggttc ttccggtgtc
      1330       1340       1350       1360       1370       1380
ggtttgaccg cagcggtcac tactgatagg gaaacaggtg aaagaagact agaggctggt
      1390       1400       1410       1420       1430       1440
gccatggttc ttgctgaccg cggggttgta tgtattgatg aatttgataa gatgacagat
      1450       1460       1470       1480       1490       1500
gtggatagag tcgccattca tgaagtaatg gaacaacaaa cggtgacgat tgccaaagca
      1510       1520       1530       1540       1550       1560
ggtattcaca caacattaaa tgctcgttgt agtgttattg ctgccgcaaa tcccgttttt
      1570       1580       1590       1600       1610       1620
gggcagtacg atgtcaatag agatccacac caaaacattg ccctaccgga ctcgctgttg
      1630       1640       1650       1660       1670       1680
tctcgttttg atttactatt tgttgtgaca gacgatatca atgaaatcag agatagatcc
      1690       1700       1710       1720       1730       1740
attagtgagc atgtcttaag aacacacaga tatttgcctc caggttattt agagggtgaa
      1750       1760       1770       1780       1790       1800
cctgtgagag agcgtttgaa tttatcatta gccgttgggg aggatgcaga tataaatcct
      1810       1820       1830       1840       1850       1860
gaagagcatt ccaactccgg ggctggtgta gaaaatgaag gagaagatga tgaagaccat
      1870       1880       1890       1900       1910       1920
gtcttcgaaa agttcaaccc cttattacaa gcaggtgcta agttagcaaa aaacaaaggt
      1930       1940       1950       1960       1970       1980
aactataacg gtacagaaat tccaaagcta gtcaccatcc cattcttaag aaagtacgtt
      1990       2000       2010       2020       2030       2040
caatatgcca aggaaagggt tattccacag ttaacacaag aagccatcaa tgttattgtg
      2050       2060       2070       2080       2090       2100
aaaaattata ctgatttaag aaacgatgat aataccaaaa aatcgcccat tactgcaaga
      2110       2120       2130       2140       2150       2160
actttggaga ctttgatcag attagccaca gctcacgcca aagtcaggtt atccaaaaca
      2170       2180       2190       2200       2210       2220
gtcaacaagg tggatgctaa agtggctgcc aatctactaa ggtttgcact attgggtgag
      2230       2240       2250       2260       2270       2280
gatatcggca atgatatcga tgaagaggaa agtgaatacg aagaagcttt gtcgaagagg
      2290       2300       2310       2320       2330       2340
tctccacaga aatcaccgaa aaaaagacaa agagtcagac aaccagcaag caactctgga
      2350       2360       2370       2380       2390       2400
tccccaatca aatctactcc aagaaggtca acggcatctt ccgttaatgc cacgccatcg
      2410       2420       2430       2440       2450       2460
tcagcacgca gaatattacg ttttcaagat gacgaacaga acgctggtga agacgataac
      2470       2480       2490       2500       2510       2520
gatataatgt caccgcttcc tgcggatgag gaagctgaat tacaaagaag gcttcaactg
      2530       2540       2550       2560       2570       2580
gggttgagag tgtctccaag acgtagagaa catcttcacg cacctgagga aggttcgtcg
      2590       2600       2610       2620       2630       2640
ggacctctta ccgaggtcgg tactccaaga ttacctaacg tatcttctgc aggtcaggat
      2650       2660       2670       2680       2690       2700
gatgagcaac aacagtcagt tatttctttt gacaatgtgg agcctggtac catttctact
      2710       2720       2730       2740       2750       2760
ggtagattgt ctttaatctc aggtattatt gcgcgtctga tgcaaacaga aatatttgaa
      2770       2780       2790       2800       2810       2820
gaagaatcct atcctgtggc ctctttgttc gaaagaatca acgaagaact accggaggag
      2830       2840       2850       2860       2870       2880
gaaaaattct ccgctcaaga atatttagca ggtttgaaga tcatgtcgga cagaaataac
      2890       2900       2910
ttaatggttg ctgacgataa agtttggaga gtctga  

Last modification date: Oct. 2, 2011