Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA replication licensing factor MCM2


DESCRIPTION:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity. Necessary for cell growth.

MISCELLANEOUS:
Present with 1690 molecules/cell in log phase SD medium.
Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex.


STRUCTURE SIMILARITY:
Contains 1 MCM domain.
Belongs to the MCM family.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD
        70         80         90        100        110        120
IDEVEEQMNE VDLMDDNMYE DYAADHNRDR YDPDQVDDRE QQELSLSERR RIDAQLNERD
       130        140        150        160        170        180
RLLRNVAYID DEDEEQEGAA QLDEMGLPVQ RRRRRRQYED LENSDDDLLS DMDIDPLREE
       190        200        210        220        230        240
LTLESLSNVK ANSYSEWITQ PNVSRTIARE LKSFLLEYTD ETGRSVYGAR IRTLGEMNSE
       250        260        270        280        290        300
SLEVNYRHLA ESKAILALFL AKCPEEMLKI FDLVAMEATE LHYPDYARIH SEIHVRISDF
       310        320        330        340        350        360
PTIYSLRELR ESNLSSLVRV TGVVTRRTGV FPQLKYVKFN CLKCGSILGP FFQDSNEEIR
       370        380        390        400        410        420
ISFCTNCKSK GPFRVNGEKT VYRNYQRVTL QEAPGTVPPG RLPRHREVIL LADLVDVSKP
       430        440        450        460        470        480
GEEVEVTGIY KNNYDGNLNA KNGFPVFATI IEANSIKRRE GNTANEGEEG LDVFSWTEEE
       490        500        510        520        530        540
EREFRKISRD RGIIDKIISS MAPSIYGHRD IKTAVACSLF GGVPKNVNGK HSIRGDINVL
       550        560        570        580        590        600
LLGDPGTAKS QILKYVEKTA HRAVFATGQG ASAVGLTASV RKDPITKEWT LEGGALVLAD
       610        620        630        640        650        660
KGVCLIDEFD KMNDQDRTSI HEAMEQQSIS ISKAGIVTTL QARCSIIAAA NPNGGRYNST
       670        680        690        700        710        720
LPLAQNVSLT EPILSRFDIL CVVRDLVDEE ADERLATFVV DSHVRSHPEN DEDREGEELK
       730        740        750        760        770        780
NNGESAIEQG EDEINEQLNA RQRRLQRQRK KEEEISPIPQ ELLMKYIHYA RTKIYPKLHQ
       790        800        810        820        830        840
MDMDKVSRVY ADLRRESIST GSFPITVRHL ESILRIAESF AKMRLSEFVS SYDLDRAIKV
       850        860
VVDSFVDAQK VSVRRQLRRS FAIYTLGH   

Encoded by MCM2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtctgata atagaagacg tagacgtgag gaagatgatt cggactcaga aaatgagcta
        70         80         90        100        110        120
ccgccatcct cacctcagca acattttaga ggggggatga acccagtttc atcacctatt
       130        140        150        160        170        180
ggttctccag atatgattaa ccctgaaggt gacgataatg aagttgacga tgtaccagat
       190        200        210        220        230        240
attgatgaag ttgaagaaca aatgaacgaa gtagatttga tggacgataa tatgtatgag
       250        260        270        280        290        300
gattatgcgg ctgatcataa tagagataga tatgatccag atcaagttga cgacagggaa
       310        320        330        340        350        360
caacaagaac tatctttaag cgaacgtcgc cgcattgatg ctcaactaaa tgaaagagat
       370        380        390        400        410        420
aggctattga gaaatgttgc ctacatagat gacgaggacg aagaacaaga aggtgcagcg
       430        440        450        460        470        480
caacttgatg aaatgggcct tcctgtgcaa agacgaagaa ggagaaggca gtatgaggac
       490        500        510        520        530        540
ttagagaata gtgatgacga tctattgagt gacatggaca ttgacccatt aagagaagaa
       550        560        570        580        590        600
cttactttag aatctctgag taacgttaag gctaacagtt actcggaatg gataacacaa
       610        620        630        640        650        660
cctaatgtct caagaactat tgccagagag ttaaaatcat tcctgctgga atatacagat
       670        680        690        700        710        720
gaaacgggtc gttctgtata tggtgcacgt attagaacat taggtgaaat gaattctgaa
       730        740        750        760        770        780
tctttggagg ttaattatag acacttagcg gagtccaaag ccatcctggc actattttta
       790        800        810        820        830        840
gctaaatgtc cagaagaaat gttgaaaata ttcgatctcg tggctatgga ggcaacagaa
       850        860        870        880        890        900
ttgcattacc cagattatgc ccgtattcac tccgaaattc acgtaagaat ctctgatttt
       910        920        930        940        950        960
ccaacaatat acagtttacg tgaattgcgt gagtctaatt tgtcctctct agtacgcgtc
       970        980        990       1000       1010       1020
actggggtgg tgacaagaag aacaggagtc ttccctcaat taaaatatgt caaattcaat
      1030       1040       1050       1060       1070       1080
tgtttgaaat gtggctccat tttgggccca ttttttcaag attctaatga agaaattaga
      1090       1100       1110       1120       1130       1140
atctcattct gtacaaactg caaatcaaaa ggtcccttta gagtcaatgg agaaaaaact
      1150       1160       1170       1180       1190       1200
gtgtaccgaa attatcaaag ggttacgctc caggaagctc ccggaaccgt tcctccaggc
      1210       1220       1230       1240       1250       1260
cgtctaccaa gacatagaga agtcattttg ttggcggatt tggtagatgt atccaagcca
      1270       1280       1290       1300       1310       1320
ggtgaagagg tagaagttac cggcatctac aaaaataact acgatggtaa cttgaatgca
      1330       1340       1350       1360       1370       1380
aagaacggat tccccgtttt tgcaacaatt atcgaggcaa attctataaa aagaagagag
      1390       1400       1410       1420       1430       1440
ggcaatacag ctaacgaagg cgaagaaggg ttggatgttt tcagttggac tgaagaagaa
      1450       1460       1470       1480       1490       1500
gaacgtgaat ttagaaagat ttctagggat cgtggtataa ttgataaaat tatatcatcg
      1510       1520       1530       1540       1550       1560
atggcaccgt ctatctatgg tcatagagat attaaaactg cagtcgcgtg ctcattattt
      1570       1580       1590       1600       1610       1620
ggaggtgttc caaaaaacgt caatggaaaa cattctattc gtggtgatat caatgtgtta
      1630       1640       1650       1660       1670       1680
ttattaggtg atccaggtac tgccaaatct caaatcttaa aatacgtcga gaaaacagcg
      1690       1700       1710       1720       1730       1740
catagagcgg tctttgcaac tggtcagggt gcttcggctg tcggtctgac agcatccgtc
      1750       1760       1770       1780       1790       1800
agaaaagatc ctattactaa agaatggacc ttagaagggg gggcgctagt attggctgat
      1810       1820       1830       1840       1850       1860
aagggtgttt gtttaattga tgaattcgat aagatgaacg atcaggatcg tacatctatt
      1870       1880       1890       1900       1910       1920
catgaggcta tggaacagca aagtatttcc atttccaagg ccggtattgt tactacatta
      1930       1940       1950       1960       1970       1980
caagcgcgct gctcaattat tgctgcggca aatcctaatg gtggtagata taattcaacc
      1990       2000       2010       2020       2030       2040
ttgcctttag ctcagaatgt tagtttgacc gagcctattc tgtctagatt tgatatttta
      2050       2060       2070       2080       2090       2100
tgtgttgtca gagaccttgt tgatgaagag gcagacgaaa gattggccac atttgttgtc
      2110       2120       2130       2140       2150       2160
gattctcatg taagatctca tccagaaaac gatgaagatc gagaaggcga agagcttaaa
      2170       2180       2190       2200       2210       2220
aataatggcg aatcggcgat agaacaagga gaagatgaaa taaatgagca gcttaatgca
      2230       2240       2250       2260       2270       2280
aggcaaagaa gacttcaaag acaaaggaag aaggaggaag agatctcgcc tattccacag
      2290       2300       2310       2320       2330       2340
gaattattga tgaaatatat tcactatgcg aggacaaaaa tataccctaa attgcatcag
      2350       2360       2370       2380       2390       2400
atggatatgg ataaggttag cagggtatat gcggatttga gaagagaaag tatttctaca
      2410       2420       2430       2440       2450       2460
ggttcgtttc caatcacagt tcgtcattta gaatctattc taagaattgc agaatctttt
      2470       2480       2490       2500       2510       2520
gctaaaatga gattatctga atttgtgtct tcttatgatt tggatagagc tatcaaagtt
      2530       2540       2550       2560       2570       2580
gtagttgatt cctttgttga tgcccaaaaa gtcagtgttc gccgccaact ccgcaggtct
      2590       2600
ttcgcaattt ataccttggg tcactaa   

Last modification date: Oct. 2, 2011