Homo sapiens L. (human) [HSA]

FULL NAME: Methylated-DNA--protein-cysteine methyltransferase


DESCRIPTION:
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

MISCELLANEOUS:
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocytalyst that acts repeatedly and over many reaction cycles.


STRUCTURE SIMILARITY:
Belongs to the MGMT family.


CATALYTIC ACTIVITY:
DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DISEASE(S):
glioma susceptibility 1 (GLM1)


RELATED DAMAGE:
O6-ethyl G (O6etG)
O6-methyl G (O6meG)
O4-methyl T (O4meT)


Amino acids sequence

        10         20         30         40         50         60
MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM
        70         80         90        100        110        120
QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL
       130        140        150        160        170        180
AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG
       190        200
LGGSSGLAGA WLKGAGATSG SPPAGRN   

Encoded by MGMT gene

FULL NAME: O-6-methylguanine-DNA methyltransferase


Nucleic acid sequence

        10         20         30         40         50         60
atgctgggac agcccgcgcc cctagaacgc tttgcgtccc gacgcccgca ggtcctcgcg
        70         80         90        100        110        120
gtgcgcaccg tttgcgactt ggtacttgga aaaatggaca aggattgtga aatgaaacgc
       130        140        150        160        170        180
accacactgg acagcccttt ggggaagctg gagctgtctg gttgtgagca gggtctgcac
       190        200        210        220        230        240
gaaataaagc tcctgggcaa ggggacgtct gcagctgatg ccgtggaggt cccagccccc
       250        260        270        280        290        300
gctgcggttc tcggaggtcc ggagcccctg atgcagtgca cagcctggct gaatgcctat
       310        320        330        340        350        360
ttccaccagc ccgaggctat cgaagagttc cccgtgccgg ctcttcacca tcccgttttc
       370        380        390        400        410        420
cagcaagagt cgttcaccag acaggtgtta tggaagctgc tgaaggttgt gaaattcgga
       430        440        450        460        470        480
gaagtgattt cttaccagca attagcagcc ctggcaggca accccaaagc cgcgcgagca
       490        500        510        520        530        540
gtgggaggag caatgagagg caatcctgtc cccatcctca tcccgtgcca cagagtggtc
       550        560        570        580        590        600
tgcagcagcg gagccgtggg caactactcc ggaggactgg ccgtgaagga atggcttctg
       610        620        630        640        650        660
gcccatgaag gccaccggtt ggggaagcca ggcttgggag ggagctcagg tctggcaggg
       670        680        690        700        710
gcctggctca agggagcggg agctacctcg ggctccccgc ctgctggccg aaactga

Last modification date: Oct. 2, 2011