Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA polymerase epsilon subunit C


DESCRIPTION:
DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

MISCELLANEOUS:
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 784 molecules/cell in log phase SD medium.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)


Amino acids sequence

        10         20         30         40         50         60
MSNLVKEKAP VFPISKVKKI AKCDPEYVIT SNVAISATAF AAELFVQNLV EESLVLAQLN
        70         80         90        100        110        120
SKGKTSLRLS LNSIEECVEK RDNFRFLEDA IKQLKKNSAL DKKRELNMQP GRSDQEVVIE
       130        140        150        160        170        180
EPELHEDDGV EEEEEEDEVS EEEEPVHNEE LLDDSKDQQN DKSTRSVASL LSRFQYKSAL
       190        200
DVGEHSDSSD IEVDHTKSTD P   

Encoded by DPB3 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtccaact tagttaaaga aaaagcacct gtctttccta tatctaaagt aaagaagatt
        70         80         90        100        110        120
gccaaatgcg accccgaata cgtaattaca tctaatgtag ctatatcagc gaccgcattc
       130        140        150        160        170        180
gctgctgagt tatttgtaca gaatctcgtc gaagaatcgc tggtcttagc acaactgaat
       190        200        210        220        230        240
tcgaaaggaa agacaagcct acgattaagc ctaaattcta tagaagaatg tgtcgaaaaa
       250        260        270        280        290        300
agagataatt tcaggtttct agaggatgcc attaaacaac tgaagaagaa tagcgcactc
       310        320        330        340        350        360
gacaagaaga gagaactaaa catgcaaccg ggtcggagcg atcaagaggt tgttatagaa
       370        380        390        400        410        420
gagcctgaat tgcatgagga tgatggtgta gaggaagaag aagaagaaga cgaggtatcc
       430        440        450        460        470        480
gaagaagaag agcccgtaca caatgaagaa cttcttgatg atagtaaaga tcaacaaaat
       490        500        510        520        530        540
gataaatcca cgcgcagtgt ggcaagcttg ctgtcgagat tccagtataa atccgcacta
       550        560        570        580        590        600
gacgtaggag aacactccga ctcttctgat atcgaagttg accatacgaa aagcaccgat

ccttag     

Last modification date: Oct. 2, 2011