Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA polymerase epsilon subunit D


DESCRIPTION:
DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair. Also functions as component of the ISW2 complex, which acts in remodeling the chromatin by catalyzing an ATP-dependent alteration in the structure of nucleosomal DNA. THe ISW2 complex is involved in coordinating transcriptional repression and in inheritance of telomeric silencing. It is involved in repression of MAT a-specific genes, INO1, and early meiotic genes during mitotic growth dependent upon transcription factor UME6 and in a parallel pathway to the RPD3-SIN3 histone deacetylase complex.

MISCELLANEOUS:
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.
Present with 2100 molecules/cell in log phase SD medium.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)


Amino acids sequence

        10         20         30         40         50         60
MPPKGWRKDA QGNYPTTSYI KEQENITIQD LLFPKSTIVN LAREVPQQSG KKLLINKDAS
        70         80         90        100        110        120
LALQRGATVF VNHLLLFARE IAKSQDKKSC SVDDVLSALD HIGHSALKGP VRDKLDEYQA
       130        140        150        160        170        180
AVEQRKKEKL DSGEVDADGD IDMGEDKENV PVEKVKEHDE IEEQGDALQD VEESSEKKQK
       190
TESQDVETRV QNLEQT    

Encoded by DPB4 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgccaccaa aaggttggag aaaagacgcc caagggaatt accccactac ttcgtatatt
        70         80         90        100        110        120
aaagaacaag agaacattac gattcaggac ctgttatttc ctaaaagcac tattgtgaat
       130        140        150        160        170        180
ttggcacgcg aagtgccaca gcaaagcggc aaaaagctgc tgataaataa agacgcatca
       190        200        210        220        230        240
ctagcgttac agcgtggtgc tacagtattt gttaaccact tgctcctgtt tgcaagggaa
       250        260        270        280        290        300
attgcaaaga gtcaagataa aaaaagctgc agtgtagatg atgttctgag tgctttggac
       310        320        330        340        350        360
catataggac actcggcatt gaagggtccg gttagagata aactagatga ataccaagct
       370        380        390        400        410        420
gctgttgaac agaggaagaa ggaaaaacta gatagtggtg aagtagatgc tgatggcgat
       430        440        450        460        470        480
atagatatgg gtgaagataa ggagaatgtg cccgtggaaa aggttaaaga gcatgacgaa
       490        500        510        520        530        540
atagaagaac aaggcgatgc attacaggac gtagaagagt cctccgaaaa gaaacagaaa
       550        560        570        580        590
acggagagcc aagatgtaga aactagagtt caaaaccttg agcaaacgta a

Last modification date: Oct. 2, 2011