Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA polymerase epsilon catalytic subunit A


DESCRIPTION:
DNA polymerase epsilon (DNA polymerase II) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

MISCELLANEOUS:
Present with 1970 molecules/cell in log phase SD medium.
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.


STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-B family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)


Amino acids sequence

        10         20         30         40         50         60
MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY
        70         80         90        100        110        120
NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG NSNDGERVTT NQGISGVDFY
       130        140        150        160        170        180
FLDEEGGSFK STVVYDPYFF IACNDESRVN DVEELVKKYL ESCLKSLQII RKEDLTMDNH
       190        200        210        220        230        240
LLGLQKTLIK LSFVNSNQLF EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE
       250        260        270        280        290        300
DIREYDVPYH VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF
       310        320        330        340        350        360
PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF NENDEVALLQ
       370        380        390        400        410        420
RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD EIGFAPDAEG EYKSSYCSHM
       430        440        450        460        470        480
DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN PIELDPELMT PYAFEKPQHL SEYSVSDAVA
       490        500        510        520        530        540
TYYLYMKYVH PFIFSLCTII PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE
       550        560        570        580        590        600
RFYDGHLLES ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN
       610        620        630        640        650        660
KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT TNRLQPDSIK
       670        680        690        700        710        720
AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI KRALQNETFP NKNKFSKKKV
       730        740        750        760        770        780
LTFDELSYAD QVIHIKKRLT EYSRKVYHRV KVSEIVEREA IVCQRENPFY VDTVKSFRDR
       790        800        810        820        830        840
RYEFKGLAKT WKGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR
       850        860        870        880        890        900
WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK
       910        920        930        940        950        960
LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY KAMILPSSKE
       970        980        990       1000       1010       1020
EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS DIFKVFLEGD TLEGCYSAVA
      1030       1040       1050       1060       1070       1080
SVCNRWLDVL DSHGLMLEDE DLVSLICENR SMSKTLKEYE GQKSTSITTA RRLGDFLGED
      1090       1100       1110       1120       1130       1140
MVKDKGLQCK YIISSKPFNA PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI
      1150       1160       1170       1180       1190       1200
IDWGYYRERL GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF
      1210       1220       1230       1240       1250       1260
SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT NEEDPLVLPS
      1270       1280       1290       1300       1310       1320
EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS RERSALGSMI RKQAESYANS
      1330       1340       1350       1360       1370       1380
TWEVLQYKDS GEPGVLEVFV TINGKVQNIT FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS
      1390       1400       1410       1420       1430       1440
ASLPNNPKTS NPAGGQLFKI TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD
      1450       1460       1470       1480       1490       1500
LGASVTFRSK AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS
      1510       1520       1530       1540       1550       1560
LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV DIKEDINFEF
      1570       1580       1590       1600       1610       1620
VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL LGTIRLLNQM PIVKLSLNEV
      1630       1640       1650       1660       1670       1680
LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL
      1690       1700       1710       1720       1730       1740
KKENIVLWWN EKAPLPDHGG IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT
      1750       1760       1770       1780       1790       1800
VNTILTSALI NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD
      1810       1820       1830       1840       1850       1860
EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE FSALGSTIVY
      1870       1880       1890       1900       1910       1920
ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL NIKRYWDLLI WMDKFNFSGL
      1930       1940       1950       1960       1970       1980
ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ PEFEDWMMII LDSMLKTKQS YLKLNSGTQR
      1990       2000       2010       2020       2030       2040
PTQIVNVKKQ DKEDSVENSL NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL
      2050       2060       2070       2080       2090       2100
PGSHLNVKNP LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS
      2110       2120       2130       2140       2150       2160
LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL RSDIESYLIQ
      2170       2180       2190       2200       2210       2220
DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV FKQVAKYYGF DILLSCIADL

TI     

Encoded by POL2 gene


OTHER NAME(S):
DUN2


Nucleic acid sequence

        10         20         30         40         50         60
atgatgtttg gcaagaaaaa aaacaacgga ggatcttcca ctgcaagata ttcagctggc
        70         80         90        100        110        120
aacaagtaca acacactctc aaataattat gcgcttagcg cgcaacagct cttaaatgct
       130        140        150        160        170        180
agtaagatcg atgacatcga ttcgatgatg ggatttgaaa gatacgtacc gccgcaatac
       190        200        210        220        230        240
aatggcaggt ttgatgcgaa ggatatagat cagattccag gccgcgtagg gtggctgacg
       250        260        270        280        290        300
aacatgcacg caacgctggt ctctcaggaa accttatcca gtggtagtaa tggcggcggc
       310        320        330        340        350        360
aattcgaatg acggagaacg tgtaacgacc aaccaaggta tttccggagt tgacttctac
       370        380        390        400        410        420
tttttagatg aagagggtgg gagcttcaag tcgacagttg tctatgaccc atacttcttt
       430        440        450        460        470        480
attgcgtgta acgatgaatc aagagtaaat gatgtggagg aactagtgaa aaaatatctg
       490        500        510        520        530        540
gaatcttgtc tcaaaagctt acaaatcatt agaaaggaag atcttaccat ggacaatcac
       550        560        570        580        590        600
cttttagggc tgcagaagac acttattaag ttatcatttg taaattccaa tcagttattc
       610        620        630        640        650        660
gaggccagga aactcctgag gccaatcttg caggataatg ccaataataa tgtgcaaaga
       670        680        690        700        710        720
aatatatata acgttgctgc aaatggctcg gaaaaagttg acgccaaaca tctgatcgaa
       730        740        750        760        770        780
gatatcaggg aatatgatgt gccgtatcat gtccgagtat ctatagacaa ggacattaga
       790        800        810        820        830        840
gtcggtaaat ggtataaggt aactcaacag ggattcattg aagatactag gaaaattgca
       850        860        870        880        890        900
tttgccgacc ctgtggtaat ggcatttgat atagaaacca cgaagccgcc tttaaaattc
       910        920        930        940        950        960
ccggattccg ccgtagatca aataatgatg atttcgtata tgatcgatgg ggaaggtttt
       970        980        990       1000       1010       1020
ttgataacaa atagggagat aatctctgag gatattgaag actttgagta tacaccgaaa
      1030       1040       1050       1060       1070       1080
ccggagtatc ctggtttttt caccatattt aacgaaaacg atgaagtggc gcttctacaa
      1090       1100       1110       1120       1130       1140
aggttttttg aacatataag agatgtacga cccactgtta tatccacctt caatggtgac
      1150       1160       1170       1180       1190       1200
tttttcgatt ggccttttat acataacaga agtaagattc acggcttgga catgttcgat
      1210       1220       1230       1240       1250       1260
gaaattggtt tcgctccaga tgctgaaggt gagtacaagt cctcatactg ctctcacatg
      1270       1280       1290       1300       1310       1320
gattgtttcc gttgggtgaa gcgtgattct tatttaccac aaggttccca gggtttaaaa
      1330       1340       1350       1360       1370       1380
gctgttactc aatctaagct aggttataac ccaattgaac tggatcccga attaatgacg
      1390       1400       1410       1420       1430       1440
ccgtatgcat ttgaaaagcc acagcacctt tccgaatatt ctgtttccga tgcagtcgct
      1450       1460       1470       1480       1490       1500
acgtattacc tttacatgaa atatgttcat ccttttatct tttccctttg tactattatt
      1510       1520       1530       1540       1550       1560
cctttgaacc cggatgaaac attgagaaag ggtaccggta ctttgtgtga aatgttgttg
      1570       1580       1590       1600       1610       1620
atggttcaag cttatcaaca taatattctt ctaccaaata agcatacaga tcccattgag
      1630       1640       1650       1660       1670       1680
aggttctatg atggacatct tctagaatcc gagacttacg tgggtggaca tgtggagtca
      1690       1700       1710       1720       1730       1740
ttagaagctg gtgtttttag gagtgatttg aagaatgaat tcaagataga tccttctgcc
      1750       1760       1770       1780       1790       1800
attgatgaat tattacaaga attaccagaa gctttgaaat ttagtgtgga agttgaaaat
      1810       1820       1830       1840       1850       1860
aagtccagtg tagataaagt aacgaatttt gaggaaataa aaaaccagat aacgcagaaa
      1870       1880       1890       1900       1910       1920
ttattagagt tgaaggaaaa caatataaga aacgaactac ctttgatcta tcatgtagat
      1930       1940       1950       1960       1970       1980
gtcgcctcta tgtacccaaa catcatgact acaaatagac tacaaccaga tagtatcaaa
      1990       2000       2010       2020       2030       2040
gcagagcgcg attgtgctag ttgcgatttt aatagacccg gaaaaacctg tgcaagaaag
      2050       2060       2070       2080       2090       2100
ttaaaatggg cttggagagg agaattcttt cccagtaaga tggatgagta taacatgatc
      2110       2120       2130       2140       2150       2160
aagcgtgcat tacaaaatga gacttttccc aacaaaaaca agttttctaa aaagaaagtt
      2170       2180       2190       2200       2210       2220
ttgacatttg atgaactaag ttacgcagac caagttatcc acataaaaaa acgtttaact
      2230       2240       2250       2260       2270       2280
gaatattcaa ggaaagttta tcatagggtt aaagtatcag aaattgtcga acgagaagcc
      2290       2300       2310       2320       2330       2340
attgtctgcc aaagagaaaa tccattctac gtcgataccg tgaaatcctt tcgtgatagg
      2350       2360       2370       2380       2390       2400
cgttacgaat tcaaaggttt agccaagact tggaagggaa atctgtccaa aattgaccca
      2410       2420       2430       2440       2450       2460
tctgataagc atgcgagaga cgaggccaaa aagatgattg tgctttatga ctcattacaa
      2470       2480       2490       2500       2510       2520
ttagctcaca aagttatttt gaattcgttt tatgggtatg ttatgaggaa aggctctcgt
      2530       2540       2550       2560       2570       2580
tggtattcca tggaaatggc ggggattacg tgtttaacag gtgccacgat cattcaaatg
      2590       2600       2610       2620       2630       2640
gcgagagctt tagtagaaag ggtaggaaga ccattagaat tagatactga tggtatttgg
      2650       2660       2670       2680       2690       2700
tgtatcttac caaaatcttt ccctgaaact tactttttta cattagaaaa tggtaaaaag
      2710       2720       2730       2740       2750       2760
ctttatctct cctacccatg ttccatgctg aattacagag ttcaccaaaa gtttacgaat
      2770       2780       2790       2800       2810       2820
caccaatacc aagaattaaa agacccattg aactatatat atgagacgca cagtgaaaac
      2830       2840       2850       2860       2870       2880
acgatttttt tcgaagttga cggaccatat aaggccatga ttttgcctag ttccaaggaa
      2890       2900       2910       2920       2930       2940
gaaggaaaag gtataaagaa aagatatgct gtcttcaatg aagacggctc acttgctgaa
      2950       2960       2970       2980       2990       3000
ctgaaaggtt ttgaattgaa gaggcgtggt gaattacaac taataaaaaa ttttcaaagt
      3010       3020       3030       3040       3050       3060
gatattttca aggtcttttt ggaaggtgat acattagaag gatgttacag tgctgtagca
      3070       3080       3090       3100       3110       3120
agcgtatgta accgttggtt agatgttctt gattcacatg gtcttatgtt agaagatgaa
      3130       3140       3150       3160       3170       3180
gacttggtca gtttgatttg tgaaaataga agtatgtcaa aaactttaaa ggaatatgaa
      3190       3200       3210       3220       3230       3240
gggcaaaaat ctacttctat tacgacggca aggagattgg gggatttttt gggtgaagat
      3250       3260       3270       3280       3290       3300
atggtaaaag ataaaggtct acaatgtaaa tatattatta gttcaaaacc tttcaatgca
      3310       3320       3330       3340       3350       3360
cctgttactg aacgagccat tccagtcgca atattttcag cggacattcc catcaaaagg
      3370       3380       3390       3400       3410       3420
tcttttctga ggcgatggac attagatcca tctttggaag atctggatat cagaaccata
      3430       3440       3450       3460       3470       3480
atcgattggg gttattatag agaaagactt ggatctgcta tacaaaagat aattactatt
      3490       3500       3510       3520       3530       3540
ccagcagcat tacaaggggt ttccaatcct gttccaaggg ttgaacatcc agattggcta
      3550       3560       3570       3580       3590       3600
aaaagaaaaa tcgctacaaa ggaggataag tttaagcaga cttcactaac caaatttttt
      3610       3620       3630       3640       3650       3660
tcgaagacaa agaatgtacc aacaatgggc aagataaaag atatcgagga tttgtttgaa
      3670       3680       3690       3700       3710       3720
ccaactgtag aagaagataa cgccaaaatt aaaattgcaa gaactactaa aaagaaagcc
      3730       3740       3750       3760       3770       3780
gtatccaaga ggaaaagaaa tcagcttaca aatgaagaag atccactagt attgccctcg
      3790       3800       3810       3820       3830       3840
gagattcctt ccatggacga ggactatgtt gggtggctaa attatcaaaa aattaaatgg
      3850       3860       3870       3880       3890       3900
aaaatccaag caagagatag aaagcgtcga gaccaattat ttggtaatac aaacagctcc
      3910       3920       3930       3940       3950       3960
cgtgaaagaa gtgcactagg aagtatgatt aggaagcaag ctgaatcata tgcgaactcc
      3970       3980       3990       4000       4010       4020
acttgggagg tcttacaata caaggattcc ggtgagccag gggttttgga agtatttgta
      4030       4040       4050       4060       4070       4080
acaattaatg gcaaagtcca gaacatcacc ttccatatac caaaaactat ttatatgaaa
      4090       4100       4110       4120       4130       4140
ttcaaatctc aaacaatgcc gctacaaaag attaagaatt gccttattga aaaatcttct
      4150       4160       4170       4180       4190       4200
gcatcgttac caaataatcc caaaacgtct aatccagcag gcggtcagct attcaaaatt
      4210       4220       4230       4240       4250       4260
actctaccgg aatctgtctt tctggaagaa aaggaaaact gcactagtat cttcaacgat
      4270       4280       4290       4300       4310       4320
gaaaatgtac ttggtgtatt tgagggcact atcactcctc atcaaagagc gatcatggat
      4330       4340       4350       4360       4370       4380
ttgggagctt cggtaacgtt ccgctcaaaa gcaatgggtg cgttaggcaa gggaatacag
      4390       4400       4410       4420       4430       4440
cagggttttg aaatgaagga tctttcaatg gcggaaaatg aaaggtatct gagtggattt
      4450       4460       4470       4480       4490       4500
tcaatggaca ttggctattt actacatttc ccaacatcaa ttgggtatga atttttttca
      4510       4520       4530       4540       4550       4560
ttattcaagt catggggaga tactattact atattagttt tgaaaccatc caaccaggct
      4570       4580       4590       4600       4610       4620
caggaaataa atgcctcatc attaggacaa atatacaaac aaatgtttga aaaaaagaaa
      4630       4640       4650       4660       4670       4680
ggtaaaatag aaacatattc ttacttggtt gatattaaag aagatatcaa ttttgagttt
      4690       4700       4710       4720       4730       4740
gtatatttta cagatatctc aaaattgtac agaagactat cacaggaaac tactaaatta
      4750       4760       4770       4780       4790       4800
aaagaagaaa gaggtctgca gtttttactc ttgttacaat ctccgtttat cactaagctc
      4810       4820       4830       4840       4850       4860
ttaggcacaa tccggcttct aaaccagatg cccattgtta agctttcctt gaatgaagtt
      4870       4880       4890       4900       4910       4920
cttctacccc aattgaactg gcaaccgaca ttattgaaga aacttgttaa ccacgtttta
      4930       4940       4950       4960       4970       4980
tccagtggtt cgtggatttc tcacttgatc aagttatccc agtatagtaa cattccaatc
      4990       5000       5010       5020       5030       5040
tgtaatttga ggctggatag tatggattat attattgatg ttctttatgc aagaaaacta
      5050       5060       5070       5080       5090       5100
aaaaaagaga acatcgtgct ttggtggaat gagaaagctc cacttccaga tcatggaggc
      5110       5120       5130       5140       5150       5160
attcaaaatg attttgattt aaatacatca tggataatga atgattcaga atttcccaaa
      5170       5180       5190       5200       5210       5220
attaataact caggtgtgta tgacaatgta gttctcgatg ttggtgttga taatttaaca
      5230       5240       5250       5260       5270       5280
gtgaacacaa ttttgacatc agcattaatc aatgatgctg aaggcagtga tctagttaac
      5290       5300       5310       5320       5330       5340
aataatatgg gtatagatga caaagatgcc gttattaact cgccatctga attcgtgcac
      5350       5360       5370       5380       5390       5400
gacgcctttt ctaatgacgc tttgaatgtt ttaagaggta tgttaaagga gtggtgggat
      5410       5420       5430       5440       5450       5460
gaggccctaa aagaaaattc aaccgcagat ttgttggtaa attccctggc aagttgggtt
      5470       5480       5490       5500       5510       5520
caaaacccga atgcgaaact attcgacgga ttactaagat atcacgttca taacttaaca
      5530       5540       5550       5560       5570       5580
aaaaaagcct tacttcaatt agtaaatgaa tttagtgcac ttggctcaac tattgtatat
      5590       5600       5610       5620       5630       5640
gcagacagga atcaaattct aataaagaca aacaagtact cacctgaaaa ctgttacgcc
      5650       5660       5670       5680       5690       5700
tacagccaat atatgatgaa ggcagttaga acaaatccaa tgtttagtta tctggactta
      5710       5720       5730       5740       5750       5760
aatatcaaac gttattggga tctgctaata tggatggata agtttaattt tagtggatta
      5770       5780       5790       5800       5810       5820
gcatgtattg aaatagagga aaaggaaaat caggattata ccgctgtttc gcaatggcaa
      5830       5840       5850       5860       5870       5880
ctaaagaagt ttctgtcacc aatatatcag cccgaatttg aggattggat gatgatcata
      5890       5900       5910       5920       5930       5940
ttggatagta tgctaaagac aaagcagagc tatctaaaat tgaattcagg gacgcaaaga
      5950       5960       5970       5980       5990       6000
cctacccaaa tagttaatgt aaaaaaacaa gataaggaag atagtgttga aaactcgttg
      6010       6020       6030       6040       6050       6060
aacggatttt ctcacctttt ttccaaacca ctaatgaaaa gagtcaaaaa gctttttaaa
      6070       6080       6090       6100       6110       6120
aaccagcaag agttcatttt agatcctcag tatgaggcag actatgttat tcctgttctt
      6130       6140       6150       6160       6170       6180
cctggttccc atctgaatgt gaaaaatccc cttctagaac ttgtcaaatc actctgccat
      6190       6200       6210       6220       6230       6240
gtcatgttac tttcaaagag tacaatttta gaaatcagga ccctgagaaa agaactgctg
      6250       6260       6270       6280       6290       6300
aagatatttg aattgcgtga gtttgctaaa gtagcggaat tcaaagatcc aagtttgagt
      6310       6320       6330       6340       6350       6360
ctcgtggtgc cggatttttt atgtgaatac tgttttttca tttctgatat tgacttttgt
      6370       6380       6390       6400       6410       6420
aaggcagctc ctgaatctat tttttcatgc gtcagatgtc acaaagcctt taatcaagta
      6430       6440       6450       6460       6470       6480
ttgttgcaag aacacctgat tcaaaaacta cgttctgata tcgaatccta tttaattcaa
      6490       6500       6510       6520       6530       6540
gatttgagat gctccagatg tcataaagtg aaacgtgact atatgagtgc ccactgtcca
      6550       6560       6570       6580       6590       6600
tgtgccggcg cgtgggaagg aactctcccc agagaaagca ttgttcaaaa gttaaatgtg
      6610       6620       6630       6640       6650       6660
tttaagcaag tagccaagta ttacggtttt gatatattat tgagttgtat tgctgatttg

accatatga     

Last modification date: Oct. 2, 2011