Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Alpha-ketoglutarate-dependent dioxygenase AlkB


DESCRIPTION:
Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents).

STRUCTURE SIMILARITY:
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.


PROTEIN TYPE(S):
dioxygenase


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
3-methylC (3meC) in ssDNA
3-methylC (3meC) in RNA
N3-methylT (3meT) in ssDNA
1,N6-etheno-A (1εA)
1-ethyl A (1etA)
3,N4-etheno-C (εC) in dsDNA
N1-methyl A (1meA)
1-methyl G (1meG)
3-ethyl C (3etC)
1,N6-etheno-A in (1εA) dsDNA
3-methyl C (3meC)
N3-methyl T (3meT)


Amino acids sequence

        10         20         30         40         50         60
MLDLFADAEP WQEPLAAGAV ILRRFAFNAA EQLIRDINDV ASQSPFRQMV TPGGYTMSVA
        70         80         90        100        110        120
MTNCGHLGWT THRQGYLYSP IDPQTNKPWP AMPQSFHNLC QRAATAAGYP DFQPDACLIN
       130        140        150        160        170        180
RYAPGAKLSL HQDKDEPDLR APIVSVSLGL PAIFQFGGLK RNDPLKRLLL EHGDVVVWGG
       190        200        210
ESRLFYHGIQ PLKAGFHPLT IDCRYNLTFR QAGKKE  

Encoded by alkB gene


OTHER NAME(S):
aidD
ECK2204
JW2200


DESCRIPTION:
AlkB mutants are extremely sensitive to MMS. [More information is available at EcoGene: EG10037]. AlkB is a Fe(II)-containing, 2-oxoglutarate-dependent dioxygenase involved in the adaptive DNA-repair response of Escherichia coli to alkylating agents. [More information is available at EcoCyc: EG10037].


Nucleic acid sequence

        10         20         30         40         50         60
atgttggatc tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggtgcggta
        70         80         90        100        110        120
attttacggc gttttgcttt taacgctgcg gagcaactga tccgcgatat taatgacgtt
       130        140        150        160        170        180
gccagccagt cgccgtttcg ccagatggtc acccccgggg gatataccat gtcggtggcg
       190        200        210        220        230        240
atgaccaact gtgggcatct gggctggacg acccatcggc aaggttatct ctattcgccc
       250        260        270        280        290        300
attgatccgc aaacaaataa accgtggccc gccatgccac agagttttca taatttatgt
       310        320        330        340        350        360
caacgtgcgg ctacggcggc gggctatcca gatttccagc cagatgcttg tcttatcaac
       370        380        390        400        410        420
cgctacgctc ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc
       430        440        450        460        470        480
gcgccaattg tttctgtttc tctgggctta cccgcgattt ttcaatttgg cggcctgaaa
       490        500        510        520        530        540
cgaaatgatc cgctcaaacg tttgttgttg gaacatggcg atgtggtggt atggggcggt
       550        560        570        580        590        600
gaatcgcggc tgttttatca cggtattcaa ccgttgaaag cggggtttca tccactcacc
       610        620        630        640        650
atcgactgcc gctacaacct gacattccgt caggcaggta aaaaagaata a

Last modification date: Oct. 2, 2011