Homo sapiens L. (human) [HSA]

FULL NAME: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3


DESCRIPTION:
Dioxygenase that repairs alkylated DNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Has a strong preference for single-stranded DNA. May also act on RNA. Requires molecular oxygen, alpha-ketoglutarate and iron.

STRUCTURE SIMILARITY:
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.


ENZYME REGULATION:
Activated by ascorbate.


PROTEIN TYPE(S):
dioxygenase


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
N1-methyl A (1meA)
3-methylC (3meC) in ssDNA
3-methyl C (3meC)
1-ethyl A (1etA)


Amino acids sequence

        10         20         30         40         50         60
MEEKRRRARV QGAWAAPVKS QAIAQPATTA KSHLHQKPGQ TWKNKEHHLS DREFVFKEPQ
        70         80         90        100        110        120
QVVRRAPEPR VIDREGVYEI SLSPTGVSRV CLYPGFVDVK EADWILEQLC QDVPWKQRTG
       130        140        150        160        170        180
IREDITYQQP RLTAWYGELP YTYSRITMEP NPHWHPVLRT LKNRIEENTG HTFNSLLCNL
       190        200        210        220        230        240
YRNEKDSVDW HSDDEPSLGR CPIIASLSFG ATRTFEMRKK PPPEENGDYT YVERVKIPLD
       250        260        270        280
HGTLLIMEGA TQADWQHRVP KEYHSREPRV NLTFRTVYPD PRGAPW 

Encoded by ALKBH3 gene

FULL NAME: alkB, alkylation repair homolog 3 (E. coli)


OTHER NAME(S):
ABH3
DEPC-1
DEPC1
FLJ43614
MGC118790
MGC118792
MGC118793
PCA1


DESCRIPTION:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggaggaaa aaagacggcg agcccgagtt cagggagcct gggctgcccc tgttaaaagc
        70         80         90        100        110        120
caggccattg ctcagccagc taccactgct aagagccatc tccaccagaa gcctggccag
       130        140        150        160        170        180
acctggaaga acaaagagca tcatctctct gacagagagt ttgtgttcaa agaacctcag
       190        200        210        220        230        240
caggtagtac gtagagctcc tgagccacga gtgattgaca gagagggtgt gtatgaaatc
       250        260        270        280        290        300
agcctgtcac ccacaggtgt atctagggtc tgtttgtatc ctggctttgt tgacgtgaaa
       310        320        330        340        350        360
gaagctgact ggatattgga acagctttgt caagatgttc cctggaaaca gaggaccggc
       370        380        390        400        410        420
atcagagagg atataactta tcagcaacca agacttacag catggtatgg agaacttcct
       430        440        450        460        470        480
tacacttatt caagaatcac tatggaacca aatcctcact ggcaccctgt gctgcgcaca
       490        500        510        520        530        540
ctaaagaacc gcattgaaga gaacactggc cacaccttca actccttact ctgcaatctt
       550        560        570        580        590        600
tatcgcaatg agaaggacag cgtggactgg cacagtgatg atgaaccctc actagggagg
       610        620        630        640        650        660
tgccccatta ttgcttcact aagttttggt gccacacgca catttgagat gagaaagaag
       670        680        690        700        710        720
ccaccaccag aagagaatgg agactacaca tatgtggaaa gagtgaagat acccttggat
       730        740        750        760        770        780
catgggacct tgttaatcat ggaaggagcg acacaagctg actggcagca tcgagtgccc
       790        800        810        820        830        840
aaagaatacc actctagaga accgagagtg aacctgacct ttcggacagt ctatccagac
       850        860
cctcgagggg caccctggtg a   

Last modification date: Oct. 2, 2011