Homo sapiens L. (human) [HSA]

FULL NAME: Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2


DESCRIPTION:
Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine (in vitro). Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.

STRUCTURE SIMILARITY:
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.


ENZYME REGULATION:
Activated by ascorbate and magnesium ions.


PROTEIN TYPE(S):
dioxygenase


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
1,N6-etheno-A (1εA)
1-ethyl A (1etA)
N1-methyl A (1meA)
3-methyl C (3meC)
1,N6-etheno-A (1εA) in ssDNA
3,N4-ethenoC (εC) in ssDNA
1,N2-etheno-G (εG) in ssDNA


Amino acids sequence

        10         20         30         40         50         60
MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI
        70         80         90        100        110        120
RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL
       130        140        150        160        170        180
TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP
       190        200        210        220        230        240
GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV
       250        260
RKKVLAPRVN LTFRKILLTK K   

Encoded by ALKBH2 gene

FULL NAME: alkB, alkylation repair homolog 2 (E. coli)


OTHER NAME(S):
ABH2
FLJ99103
MGC90512


DESCRIPTION:
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggacagat tcctggtgaa aggggctcaa gggggccttt tgaggaagca ggaggagcaa
        70         80         90        100        110        120
gagccaactg gagaagagcc agctgtgttg ggaggagaca aagaaagcac aaggaagagg
       130        140        150        160        170        180
cccaggagag aggccccagg gaatggaggc cactcagcag gccctagctg gcggcacatt
       190        200        210        220        230        240
cgggctgagg gcctggactg cagttacaca gtcctgtttg gcaaagctga ggcagatgag
       250        260        270        280        290        300
attttccaag agttggagaa agaagtagaa tattttacag gagcactggc cagagtccag
       310        320        330        340        350        360
gtattcggga agtggcacag tgtgcccagg aagcaggcaa cgtatggcga cgctgggctg
       370        380        390        400        410        420
acctacacat tttcaggcct cacgctgtct ccaaagccct ggatcccagt tctagagcgc
       430        440        450        460        470        480
atccgggatc acgtctctgg ggtgactgga cagaccttca actttgtgct catcaacagg
       490        500        510        520        530        540
tataaagatg gctgtgacca catcggggag caccgagatg atgaaagaga actggcccct
       550        560        570        580        590        600
gggagcccca ttgcctctgt ctccttcggt gcctgcagag actttgtctt ccggcataag
       610        620        630        640        650        660
gattcccgtg ggaaaagccc ctccaggagg gtggcggtgg tcaggctgcc gctggcccac
       670        680        690        700        710        720
gggagcttac taatgatgaa ccacccgacc aacacgcact ggtaccacag tcttcccgtg
       730        740        750        760        770        780
agaaagaagg ttctggctcc acgggtgaat ctgacttttc gtaaaatttt gcttactaaa

aaataa     

Last modification date: Oct. 2, 2011