Homo sapiens L. (human) [HSA]

FULL NAME: Alkylated DNA repair protein alkB homolog 1


DESCRIPTION:
Dioxygenase that repairs alkylated single-stranded DNA and RNA containing 3-methylcytosine by oxidative demethylation. Requires molecular oxygen, alpha-ketoglutarate and iron. May have a role in placental trophoblast lineage differentiation (By similarity). Has DNA lyase activity and introduces double-stranded breaks at abasic sites. Cleaves both single-stranded DNA and double-stranded DNA at abasic sites, with the greatest activity towards double-stranded DNA with two abasic sites. DNA lyase activity does not require alpha-ketoglutarate and iron.

STRUCTURE SIMILARITY:
Belongs to the alkB family.
Contains 1 Fe2OG dioxygenase domain.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


PROTEIN TYPE(S):
dioxygenase


RELATED PATHWAY(S):
direct reversal (DR)


RELATED DAMAGE:
3-methylC (3meC) in ssDNA


Amino acids sequence

        10         20         30         40         50         60
MGKMAAAVGS VATLATEPGE DAFRKLFRFY RQSRPGTADL EGVIDFSAAH AARGKGPGAQ
        70         80         90        100        110        120
KVIKSQLNVS SVSEQNAYRA GLQPVSKWQA YGLKGYPGFI FIPNPFLPGY QWHWVKQCLK
       130        140        150        160        170        180
LYSQKPNVCN LDKHMSKEET QDLWEQSKEF LRYKEATKRR PRSLLEKLRW VTVGYHYNWD
       190        200        210        220        230        240
SKKYSADHYT PFPSDLGFLS EQVAAACGFE DFRAEAGILN YYRLDSTLGI HVDRSELDHS
       250        260        270        280        290        300
KPLLSFSFGQ SAIFLLGGLQ RDEAPTAMFM HSGDIMIMSG FSRLLNHAVP RVLPNPEGEG
       310        320        330        340        350        360
LPHCLEAPLP AVLPRDSMVE PCSMEDWQVC ASYLKTARVN MTVRQVLATD QNFPLEPIED
       370        380
EKRDISTEGF CHLDDQNSEV KRARINPDS   

Encoded by ALKBH1 gene

FULL NAME: alkB, alkylation repair homolog 1 (E. coli)


OTHER NAME(S):
ABH
ABH1
ALKBH
alkB
hABH


DESCRIPTION:
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggggaaga tggcagcggc cgtgggctct gtggcgactc tggcgactga gcccggggag
        70         80         90        100        110        120
gacgcctttc ggaaactttt ccgcttctac cgtcagagcc ggcccgggac cgcagacctg
       130        140        150        160        170        180
gaaggggtca tcgacttctc ggcggcccac gcagcccgtg gcaagggtcc tggtgcccaa
       190        200        210        220        230        240
aaggtgatca aatctcagct aaatgtgtct tctgtcagtg agcagaatgc atatagagca
       250        260        270        280        290        300
ggtcttcagc ccgtcagcaa gtggcaagcc tatggactca aaggctatcc tgggtttatt
       310        320        330        340        350        360
tttatcccaa accccttcct cccaggttac cagtggcact gggtgaaaca gtgccttaag
       370        380        390        400        410        420
ttatattccc agaaacctaa tgtatgtaac ctggacaaac acatgtctaa agaagagacc
       430        440        450        460        470        480
caagatctgt gggaacagag caaagagttc ctgaggtata aagaagcgac taaacggaga
       490        500        510        520        530        540
ccccgaagtt tactggagaa actgcgttgg gtgaccgtag gctaccatta taactgggac
       550        560        570        580        590        600
agtaagaaat actcagcaga tcattacaca cctttccctt ctgacctggg tttcctctca
       610        620        630        640        650        660
gagcaagtag ccgctgcctg tggatttgag gatttccgag ctgaagcagg gatcctgaat
       670        680        690        700        710        720
tactaccgcc tggactccac actgggaatc cacgtagaca gatctgagct agatcactcc
       730        740        750        760        770        780
aaacccttgc tgtcattcag ctttggacag tccgccatct ttctcctggg tggtcttcaa
       790        800        810        820        830        840
agggatgagg cccccacggc catgtttatg cacagtggtg acatcatgat aatgtcgggt
       850        860        870        880        890        900
ttcagccgcc tcttgaacca cgcagtccct cgtgtccttc caaatccaga aggggaaggc
       910        920        930        940        950        960
ctgcctcact gcctagaggc acctctccct gctgtcctcc cgagagattc aatggtagag
       970        980        990       1000       1010       1020
ccttgttcta tggaggactg gcaggtgtgt gccagctact tgaagaccgc tcgtgttaac
      1030       1040       1050       1060       1070       1080
atgactgtcc gacaggtcct ggccacagac cagaatttcc ctctagaacc catcgaggat
      1090       1100       1110       1120       1130       1140
gaaaaaagag acatcagtac agaaggtttc tgccatctgg atgaccagaa tagcgaagta
      1150       1160       1170
aaacgggcca ggataaaccc tgacagctga   

Last modification date: Oct. 2, 2011