Homo sapiens L. (human) [HSA]

FULL NAME: 5'-dRP lyase Ku70


DESCRIPTION:
Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.
Subcellular location - Nucleus. Chromosome.

MISCELLANEOUS:
DNA-dependent protein kinase-DNA ligase 4 complex, Ku70:Ku80 complex, nuclear telomere cap complex, transcription factor complex


STRUCTURE SIMILARITY:
Belongs to the ku70 family. Contains 1 Ku domain. Contains 1 SAP domain.


SUBUNIT STRUCTURE:
Heterodimer of a 70 kDa (XRCC6) and a 80 kDa (XRCC5) subunit. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interacts with XRCC6BP1. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Interacts with CLU (By similarity). Binds to CDK9 isoform 2.


CATALYTIC ACTIVITY:
5'-deoxyribose-5-phosphate lyase activity, ATP binding, ATP-dependent DNA helicase activity, double-stranded DNA binding, protein C-terminus binding, transcription regulatory region DNA binding,


POST-TRANSLATIONAL MODIFICATION:
Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.


PROTEIN TYPE(S):
3'-5' DNA helicase
DNase
dRP-lyase


RELATED PATHWAY(S):
non-homologous end-joining (NHEJ)


RELATED DISEASE(S):
systemic lupus erythematosus (SLE)


Amino acids sequence

        10         20         30         40         50         60
MSGWESYYKT EGDEEAEEEQ EENLEASGDY KYSGRDSLIF LVDASKAMFE SQSEDELTPF
        70         80         90        100        110        120
DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK NIYVLQELDN PGAKRILELD
       130        140        150        160        170        180
QFKGQQGQKR FQDMMGHGSD YSLSEVLWVC ANLFSDVQFK MSHKRIMLFT NEDNPHGNDS
       190        200        210        220        230        240
AKASRARTKA GDLRDTGIFL DLMHLKKPGG FDISLFYRDI ISIAEDEDLR VHFEESSKLE
       250        260        270        280        290        300
DLLRKVRAKE TRKRALSRLK LKLNKDIVIS VGIYNLVQKA LKPPPIKLYR ETNEPVKTKT
       310        320        330        340        350        360
RTFNTSTGGL LLPSDTKRSQ IYGSRQIILE KEETEELKRF DDPGLMLMGF KPLVLLKKHH
       370        380        390        400        410        420
YLRPSLFVYP EESLVIGSST LFSALLIKCL EKEVAALCRY TPRRNIPPYF VALVPQEEEL
       430        440        450        460        470        480
DDQKIQVTPP GFQLVFLPFA DDKRKMPFTE KIMATPEQVG KMKAIVEKLR FTYRSDSFEN
       490        500        510        520        530        540
PVLQQHFRNL EALALDLMEP EQAVDLTLPK VEAMNKRLGS LVDEFKELVY PPDYNPEGKV
       550        560        570        580        590        600
TKRKHDNEGS GSKRPKVEYS EEELKTHISK GTLGKFTVPM LKEACRAYGL KSGLKKQELL

EALTKHFQD     

Encoded by XRCC6 gene

FULL NAME: X-ray repair complementing defective repair in Chinese hamster cells 6


OTHER NAME(S):
CTC75
CTCBF
G22P1
KU70
ML8
TLAA


DESCRIPTION:
The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atgtcagggt gggagtcata ttacaaaacc gagggcgatg aagaagcaga ggaagaacaa
        70         80         90        100        110        120
gaagagaacc ttgaagcaag tggagactat aaatattcag gaagagatag tttgattttt
       130        140        150        160        170        180
ttggttgatg cctccaaggc tatgtttgaa tctcagagtg aagatgagtt gacacctttt
       190        200        210        220        230        240
gacatgagca tccagtgtat ccaaagtgtg tacatcagta agatcataag cagtgatcga
       250        260        270        280        290        300
gatctcttgg ctgtggtgtt ctatggtacc gagaaagaca aaaattcagt gaattttaaa
       310        320        330        340        350        360
aatatttacg tcttacagga gctggataat ccaggtgcaa aacgaattct agagcttgac
       370        380        390        400        410        420
cagtttaagg ggcagcaggg acaaaaacgt ttccaagaca tgatgggcca cggatctgac
       430        440        450        460        470        480
tactcactca gtgaagtgct gtgggtctgt gccaacctct ttagtgatgt ccaattcaag
       490        500        510        520        530        540
atgagtcata agaggatcat gctgttcacc aatgaagaca acccccatgg caatgacagt
       550        560        570        580        590        600
gccaaagcca gccgggccag gaccaaagcc ggtgatctcc gagatacagg catcttcctt
       610        620        630        640        650        660
gacttgatgc acctgaagaa acctgggggc tttgacatat ccttgttcta cagagatatc
       670        680        690        700        710        720
atcagcatag cagaggatga ggacctcagg gttcactttg aggaatccag caagctagaa
       730        740        750        760        770        780
gacctgttgc ggaaggttcg cgccaaggag accaggaagc gagcactcag caggttaaag
       790        800        810        820        830        840
ctgaagctca acaaagatat agtgatctct gtgggcattt ataatctggt ccagaaggct
       850        860        870        880        890        900
ctcaagcctc ctccaataaa gctctatcgg gaaacaaatg aaccagtgaa aaccaagacc
       910        920        930        940        950        960
cggaccttta atacaagtac aggcggtttg cttctgccta gcgataccaa gaggtctcag
       970        980        990       1000       1010       1020
atctatggga gtcgtcagat tatactggag aaagaggaaa cagaagagct aaaacggttt
      1030       1040       1050       1060       1070       1080
gatgatccag gtttgatgct catgggtttc aagccgttgg tactgctgaa gaaacaccat
      1090       1100       1110       1120       1130       1140
tacctgaggc cctccctgtt cgtgtaccca gaggagtcgc tggtgattgg gagctcaacc
      1150       1160       1170       1180       1190       1200
ctgttcagtg ctctgctcat caagtgtctg gagaaggagg ttgcagcatt gtgcagatac
      1210       1220       1230       1240       1250       1260
acaccccgca ggaacatccc tccttatttt gtggctttgg tgccacagga agaagagttg
      1270       1280       1290       1300       1310       1320
gatgaccaga aaattcaggt gactcctcca ggcttccagc tggtcttttt accctttgct
      1330       1340       1350       1360       1370       1380
gatgataaaa ggaagatgcc ctttactgaa aaaatcatgg caactccaga gcaggtgggc
      1390       1400       1410       1420       1430       1440
aagatgaagg ctatcgttga gaagcttcgc ttcacataca gaagtgacag ctttgagaac
      1450       1460       1470       1480       1490       1500
cccgtgctgc agcagcactt caggaacctg gaggccttgg ccttggattt gatggagccg
      1510       1520       1530       1540       1550       1560
gaacaagcag tggacctgac attgcccaag gttgaagcaa tgaataaaag actgggctcc
      1570       1580       1590       1600       1610       1620
ttggtggatg agtttaagga gcttgtttac ccaccagatt acaatcctga agggaaagtt
      1630       1640       1650       1660       1670       1680
accaagagaa aacacgataa tgaaggttct ggaagcaaaa ggcccaaggt ggagtattca
      1690       1700       1710       1720       1730       1740
gaagaggagc tgaagaccca catcagcaag ggtacgctgg gcaagttcac tgtgcccatg
      1750       1760       1770       1780       1790       1800
ctgaaagagg cctgccgggc ttacgggctg aagagtgggc tgaagaagca ggagctgctg
      1810       1820       1830
gaagccctca ccaagcactt ccaggactga   

Last modification date: Feb. 6, 2012