Drosophila melanogaster Meigen (fruit fly) [DME]

FULL NAME: DNA topoisomerase 3-alpha


DESCRIPTION:
Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.

MISCELLANEOUS:
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand (By similarity).


STRUCTURE SIMILARITY:
Contains 1 Toprim domain.
Belongs to the prokaryotic type I/III topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


PROTEIN TYPE(S):
endonuclease
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MKAVLTCFRP VVASHRRYCA NPGQAMKYLN VAEKNDAAKT IAGLLSNGAA QRREGYSVYN
        70         80         90        100        110        120
KVFDFEAPVR GQNAKMVMTS VSGHMMQLAF QVSYKNWRTV DPRSLFDAPV KKGVGSDYEP
       130        140        150        160        170        180
IKRTLEREVR GCQGLIIWTD CDREGENIGY EIIDVCRAIK PNISVYRATF SEITTVAVRR
       190        200        210        220        230        240
ALQQLGQPDK RQSDAVDVRT ELDLRTGAAI TRFQTMRLQR LFPEKIADKL ISYGSCQIPT
       250        260        270        280        290        300
LGFVAERYKE IEAFVSEPFW KIKVLHTIDD LTVEFNWARN RLFDKEACEN YLLLCLAEPD
       310        320        330        340        350        360
PRALVESVTV KPKHKWRPTP LDTVEMEKLG SRKLKLSAKE TMTIAEKLYT KGFISYPRTE
       370        380        390        400        410        420
TNQFSKEFAL APLVEMQTGH RDWGAFAQRV IEWGPNPRNG NKSDQAHPPI HPTKLAENLQ
       430        440        450        460        470        480
GNEARVYELV VRHFLACVSK DAVGSETLVH IDIAGEKFTA NGLVIHERNY LDVYVYDKWS
       490        500        510        520        530        540
AKQIHHYENG QRFEPTEVSL HEGATTAPPL LTEADLIALM EKHGIGTDAT HAEHINTIKE
       550        560        570        580        590        600
RGYIGVLDKG FLVPGVIGMG LYEGYDAMEL ALAKPQLRAE FELDLKLICQ GQKDPKVVLT
       610        620        630        640        650        660
EQIAKYKQAY QQITDKITAM DAKISARINE TPAANSAVQE GADGSAPSHG IIQSIFQCPK
       670        680        690        700        710        720
CNEAPLALKP KKNQQGWYIG CNNFPDCKNA VWLPTECKDA SVLDECCPTC GDGYRMLKFR
       730        740        750        760        770        780
LSTPYYRGVF GTPSGWYKTC LPCDNLFRTT FNINLDSVKK VGGIVGEVRG GGGGPGPGPG
       790        800        810        820        830        840
GGGSGRGAGS GGWSSGPGGG GSGGGGGSGG WGSGTGGGGS GGWGSGTGGG GLGGGKGKKP
       850        860        870        880        890        900
GGESKKSATK KPPNEPKPKK TKEPKAAPNK KTSSKSSGSI RSFFTSAAPT NSASNGLDEF
       910        920        930        940        950        960
FDSNDGFEDA MLAAAESVES SSQPKTISMV PLDDDIAAAF AADDDAEFEA LVNGGTMPTE
       970        980        990       1000       1010       1020
SNGDQQLDKS LSEWIKEQDK ADERPMLWGT RERASLGTAA PTPPPKPAAK RPRWDSVERD
      1030       1040       1050       1060       1070       1080
STPPSSVPES ETVLCTGCQQ PARQNTVRKN GPNLGRLYYK CPKPDECNFF QWADEPPSSA
      1090       1100       1110       1120       1130       1140
KSKNSTGSAP QSTTSWGSNR VVTLPSIQQS NSQRGQSSMR SNSSSTVTIT QTKTKQQERN
      1150       1160       1170       1180       1190       1200
TATPGDGEEV MCNCGQLASQ LTVRKDGPNQ GRPFYACPTR EKSCGFFKWG DEDQNQGASS
      1210       1220       1230       1240       1250
TSWGSANRNP PGRSQPTAIT SDGPKTRRCG LCRKEGHTRN KCPRKDEFDM 

Encoded by Top3alpha gene

FULL NAME: Topoisomerase 3alpha


OTHER NAME(S):
CG10123
Dmel\CG10123
DmTop3alpha
dTopoIIIa
Top3a
top3alpha
Topo IIIalpha


Nucleic acid sequence

        10         20         30         40         50         60
atgaaagctg tcttaacatg ttttcgcccc gtcgtcgcct cacacagaag atattgcgct
        70         80         90        100        110        120
aatcccggcc aggcaatgaa gtacttaaac gtggctgaga agaacgacgc cgccaaaacg
       130        140        150        160        170        180
attgccggcc tgctgtcaaa tggcgcagca caaaggcgcg aaggctactc ggtgtacaac
       190        200        210        220        230        240
aaggtcttcg atttcgaggc ccctgtgcgc ggtcagaatg ccaaaatggt tatgacctcc
       250        260        270        280        290        300
gtatccggcc acatgatgca gctggcattc caggtgtctt acaaaaattg gagaacggtg
       310        320        330        340        350        360
gatccgcgct ctttattcga cgcgccggtg aaaaagggcg tgggctcgga ttatgagcct
       370        380        390        400        410        420
atcaaaagga ccttggaacg cgaggtgcgc ggctgtcagg gattgattat ctggacggat
       430        440        450        460        470        480
tgcgatcgcg agggcgagaa cattggctac gagattattg atgtgtgtcg cgctatcaaa
       490        500        510        520        530        540
ccgaatattt cggtttatcg tgccactttc tcggagatta ccacggtggc ggtgcgtcgg
       550        560        570        580        590        600
gctctgcagc aattggggca gccggacaaa aggcagagcg atgcagtgga tgtgcgcacg
       610        620        630        640        650        660
gaactggatt tgcgaactgg tgccgccatc acccggtttc agacaatgcg cctacaacgt
       670        680        690        700        710        720
ctgttccccg aaaagatagc tgataagctc atatcctatg gaagctgcca aattcccacc
       730        740        750        760        770        780
ctgggattcg tcgccgaacg gtacaaggag attgaggcct tcgtatccga gccattttgg
       790        800        810        820        830        840
aagattaagg ttctgcacac aattgatgac ttgacggtgg agtttaattg ggcccgaaac
       850        860        870        880        890        900
cgactcttcg ataaggaagc ttgtgaaaac tatttgctcc tctgcctggc cgaacccgat
       910        920        930        940        950        960
cctagagcgc tcgtggagag cgtcaccgtt aagcccaaac acaaatggcg cccaactccg
       970        980        990       1000       1010       1020
ctggataccg tggaaatgga gaagcttggc tcgaggaaac tcaagctatc tgccaaggag
      1030       1040       1050       1060       1070       1080
acgatgacaa tagccgaaaa attatatacc aagggtttca tcagctatcc ccgtacggaa
      1090       1100       1110       1120       1130       1140
accaaccagt tctccaagga gttcgctctg gcaccactag ttgagatgca aacgggtcac
      1150       1160       1170       1180       1190       1200
cgcgactggg gagcatttgc ccagcgggtg attgagtggg gaccaaatcc tcgaaacggt
      1210       1220       1230       1240       1250       1260
aacaaatcgg atcaggccca tcctccaatt catcccacca agctggcaga aaacctgcag
      1270       1280       1290       1300       1310       1320
ggaaacgagg cgcgagtgta cgaactggtc gtccggcact tcctcgcctg cgtcagcaaa
      1330       1340       1350       1360       1370       1380
gatgcagtcg gctccgaaac ccttgtccat atcgacattg ccggtgagaa gttcacggcc
      1390       1400       1410       1420       1430       1440
aatggtctgg ttattcacga acgaaactac ctagatgttt atgtttatga caagtggagt
      1450       1460       1470       1480       1490       1500
gccaagcaga tccatcacta tgaaaacggg cagcgttttg agcccacgga agtgtcgctc
      1510       1520       1530       1540       1550       1560
cacgaaggtg ccaccactgc cccgcccctg ctgaccgaag cggatttgat tgcactgatg
      1570       1580       1590       1600       1610       1620
gaaaagcatg ggattggtac agatgccacg cacgctgagc acataaacac gatcaaggaa
      1630       1640       1650       1660       1670       1680
cgcggataca tcggagtgct ggataagggc tttttggtac ctggagttat tggaatggga
      1690       1700       1710       1720       1730       1740
ctgtatgagg gatacgatgc catggagctg gcccttgcca agccacaact tcgtgctgag
      1750       1760       1770       1780       1790       1800
tttgaattgg atctgaaact aatttgccag gggcagaaag atcccaaggt ggtcctgacc
      1810       1820       1830       1840       1850       1860
gaacagatag ccaagtacaa gcaggcctat cagcagatta ccgataagat taccgccatg
      1870       1880       1890       1900       1910       1920
gacgccaaga tatcggctcg cattaatgag acaccagctg ccaattctgc ggtgcaggaa
      1930       1940       1950       1960       1970       1980
ggagctgatg gaagtgctcc aagtcacggc atcatccaat cgatcttcca gtgccctaag
      1990       2000       2010       2020       2030       2040
tgcaatgagg ctcctctggc cctaaagcca aagaagaacc agcagggctg gtacattggc
      2050       2060       2070       2080       2090       2100
tgcaataact ttcccgactg caaaaacgca gtttggcttc caacggagtg caaggacgca
      2110       2120       2130       2140       2150       2160
tctgtgctgg acgagtgctg tcccacctgt ggcgatggat accggatgct caagttccgc
      2170       2180       2190       2200       2210       2220
ctgagcacgc cttactatcg cggggtattt ggtactccca gcggttggta caaaacctgc
      2230       2240       2250       2260       2270       2280
ttgccctgcg ataatctctt tcgcaccacg ttcaacatta atttggactc ggttaagaaa
      2290       2300       2310       2320       2330       2340
gtgggaggca ttgtgggtga agtgcgtggt ggaggagggg gtccaggccc aggacccggt
      2350       2360       2370       2380       2390       2400
ggcggtggct ctggcagagg agccggctct ggtggatgga gcagcggacc tggaggcggt
      2410       2420       2430       2440       2450       2460
ggctctggcg gaggcggcgg ctctggagga tggggcagcg gaactggagg cggtggctct
      2470       2480       2490       2500       2510       2520
ggaggatggg gcagcggaac tggaggcggt ggcttgggcg gaggaaaggg caagaaacct
      2530       2540       2550       2560       2570       2580
ggcggcgaat ctaaaaaatc agctacaaaa aaacctccga acgaacccaa accgaaaaag
      2590       2600       2610       2620       2630       2640
acgaaagaac cgaaagcggc acccaacaaa aagactagca gcaaatcgtc tggcagcata
      2650       2660       2670       2680       2690       2700
cggagcttct ttacaagtgc ggcgcctaca aattccgcca gcaatggact agatgagttt
      2710       2720       2730       2740       2750       2760
ttcgatagta acgatggctt tgaggacgcc atgttggctg ctgccgaatc cgtggaatcg
      2770       2780       2790       2800       2810       2820
agtagccaac ccaaaaccat tagtatggtc cctcttgatg acgacatagc cgccgctttt
      2830       2840       2850       2860       2870       2880
gcagcggatg atgatgctga attcgaggcg ttggtaaacg gcggaaccat gcccaccgaa
      2890       2900       2910       2920       2930       2940
tccaatgggg accagcagct ggacaaaagt ctctcggaat ggataaagga gcaggacaaa
      2950       2960       2970       2980       2990       3000
gcggacgaga ggcccatgct ttggggaact cgggagcgag cgtcgttagg tactgctgct
      3010       3020       3030       3040       3050       3060
cccactcctc ctccgaaacc ggcagctaaa cgaccgagat gggacagtgt cgaacgggat
      3070       3080       3090       3100       3110       3120
tccacaccac cctcttcggt tccggaatcc gaaacagtgc tgtgtaccgg atgtcagcag
      3130       3140       3150       3160       3170       3180
ccagctcgcc agaacactgt ccgtaagaat ggacccaacc taggcaggct gtactacaag
      3190       3200       3210       3220       3230       3240
tgtcccaaac cggatgagtg caacttcttt cagtgggctg atgagccacc gtcgtcggcc
      3250       3260       3270       3280       3290       3300
aaaagcaaga attccaccgg cagtgcacca caaagcacaa catcctgggg ctccaatcga
      3310       3320       3330       3340       3350       3360
gtggttaccc tgcctagcat tcagcagagc aactcacagc gtggccaatc atctatgcga
      3370       3380       3390       3400       3410       3420
tcaaactcca gcagtactgt caccattact caaacgaaaa ccaaacagca ggagaggaat
      3430       3440       3450       3460       3470       3480
acagcgactc caggcgacgg agaggaggtg atgtgcaact gtgggcagtt ggctagccag
      3490       3500       3510       3520       3530       3540
ctgacggtcc gaaaagacgg accaaaccag ggtagaccct tttatgcctg tcccacgcgg
      3550       3560       3570       3580       3590       3600
gagaagtcat gcggtttctt caaatgggga gatgaggacc aaaaccaagg tgctagcagc
      3610       3620       3630       3640       3650       3660
acctcttggg gctcggctaa tcgaaatccc cctggacgca gtcagcccac agctattacg
      3670       3680       3690       3700       3710       3720
tccgatggtc ccaaaacgag acgctgtggt ctctgtcgca aggagggaca cacaaggaac
      3730       3740       3750
aagtgtccac gcaaggatga gtttgatatg tag  

Last modification date: Oct. 2, 2011