Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA primase large subunit


DESCRIPTION:
DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.

STRUCTURE SIMILARITY:
Belongs to the eukaryotic-type primase large subunit family.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL YARLYESKLS
        70         80         90        100        110        120
FYDLPPQGEI TLEQFEIWAI DRLKILLEIE SCLSRNKSIK EIETIIKPQF QKLLPFNTES
       130        140        150        160        170        180
LEDRKKDYYS HFILRLCFCR SKELREKFVR AETFLFKIRF NMLTSTDQTK FVQSLDLPLL
       190        200        210        220        230        240
QFISNEEKAE LSHQLYQTVS ASLQFQLNLN EEHQRKQYFQ QEKFIKLPFE NVIELVGNRL
       250        260        270        280        290        300
VFLKDGYAYL PQFQQLNLLS NEFASKLNQE LIKTYQYLPR LNEDDRLLPI LNHLSSGYTI
       310        320        330        340        350        360
ADFNQQKANQ FSENVDDEIN AQSVWSEEIS SNYPLCIKNL MEGLKKNHHL RYYGRQQLSL
       370        380        390        400        410        420
FLKGIGLSAD EALKFWSEAF TRNGNMTMEK FNKEYRYSFR HNYGLEGNRI NYKPWDCHTI
       430        440        450        460        470        480
LSKPRPGRGD YHGCPFRDWS HERLSAELRS MKLTQAQIIS VLDSCQKGEY TIACTKVFEM
       490        500        510        520
THNSASADLE IGEQTHIAHP NLYFERSRQL QKKQQKLEKE KLFNNGNH 

Encoded by PRI2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgttcaggc agtcaaaaag aagaattgcc tcaaggaaga attttagttc atacgatgat
        70         80         90        100        110        120
attgtcaaaa gcgaattgga cgtaggcaac acaaatgcag ctaaccagat catcttaagt
       130        140        150        160        170        180
tcaagttcat ctgaagaaga gaaaaaactt tacgctaggc tgtatgagtc taaattatca
       190        200        210        220        230        240
ttttacgatt taccaccaca gggagaaata acgttggaac aatttgagat ctgggcaatc
       250        260        270        280        290        300
gatcgtctca aaattctgtt agagatcgaa tcttgccttt caagaaacaa atcgatcaaa
       310        320        330        340        350        360
gaaattgaaa ccattatcaa gccgcagttc caaaaactac tgcctttcaa cacagagagt
       370        380        390        400        410        420
ttagaagaca gaaaaaagga ttactactct catttcattt taagattgtg cttttgtcgt
       430        440        450        460        470        480
tcaaaagaac tgcgggaaaa attcgtcagg gctgaaacgt ttttgttcaa aattagattt
       490        500        510        520        530        540
aacatgctaa cctccaccga tcaaacaaaa tttgttcaat cattggactt acccttacta
       550        560        570        580        590        600
cagtttatct caaatgaaga aaaggccgaa ttatctcatc agttgtatca aacagtttct
       610        620        630        640        650        660
gcgtctctac agttccaatt gaatttaaac gaggaacatc aaagaaaaca gtatttccaa
       670        680        690        700        710        720
caggaaaaat tcataaaatt acctttcgaa aatgtgatag aactggtagg aaaccgttta
       730        740        750        760        770        780
gtgtttttga aggacgggta cgcatattta ccacaattcc agcaattgaa tttactttct
       790        800        810        820        830        840
aatgagtttg ctagcaaatt gaaccaggag ttaataaaaa cgtaccagta cttaccaaga
       850        860        870        880        890        900
ctgaatgagg atgaccggtt gttaccaatt ctaaatcatc tttcgtcggg gtacactatc
       910        920        930        940        950        960
gcggatttca accagcaaaa ggcaaaccaa ttcagtgaga acgtagatga tgagattaat
       970        980        990       1000       1010       1020
gcgcaaagtg tctggtctga agagattagc tcaaactatc cgctatgtat caaaaacctg
      1030       1040       1050       1060       1070       1080
atggagggtc ttaaaaagaa ccatcatttg aggtattatg ggagacaaca actgagtctg
      1090       1100       1110       1120       1130       1140
tttttgaaag gtatcggcct gagcgctgat gaagcgttaa aattttggtc tgaggcattt
      1150       1160       1170       1180       1190       1200
acaagaaatg ggaacatgac aatggagaag ttcaataaag aataccgtta cagcttcagg
      1210       1220       1230       1240       1250       1260
cataattacg gtcttgaagg taacagaatc aactacaaac catgggactg tcacactatc
      1270       1280       1290       1300       1310       1320
ctttccaagc ccagacctgg gcgcggagat tatcatggat gccctttccg tgattggagt
      1330       1340       1350       1360       1370       1380
cacgagagac tatctgcaga attgcgctct atgaagttga cccaagcgca gatcatcagt
      1390       1400       1410       1420       1430       1440
gttctggatt cctgccagaa aggtgaatac acaattgctt gcactaaagt gtttgaaatg
      1450       1460       1470       1480       1490       1500
acacacaact ctgcatcagc ggatttggaa attggcgagc aaacgcatat cgcgcatcct
      1510       1520       1530       1540       1550       1560
aacctatact tcgaaagatc aaggcaactg caaaagaaac agcagaagct ggaaaaggaa
      1570       1580
aaactattca ataatggtaa tcattag   

Last modification date: Oct. 2, 2011