Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA primase small subunit


DESCRIPTION:
DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. In a complex with DNA polymerase alpha (DNA polymerase alpha:primase) constitutes a replicative polymerase. Both primase components participate in formation of the active center, but the ATP-binding site is exclusively located on p48.

MISCELLANEOUS:
Present with 197 molecules/cell in log phase SD medium.
The bound zinc ion is not a cofactor. It is bound to a zinc knuckle motif that may be involved in sequence recognition and the binding of ssDNA (By similarity).


STRUCTURE SIMILARITY:
Belongs to the eukaryotic-type primase small subunit family.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MTNSVKTNGP SSSDMEYYYK SLYPFKHIFN WLNHSPKPSR DMINREFAMA FRSGAYKRYN
        70         80         90        100        110        120
SFNSVQDFKA QIEKANPDRF EIGAIYNKPP RERDTLLKSE LKALEKELVF DIDMDDYDAF
       130        140        150        160        170        180
RTCCSGAQVC SKCWKFISLA MKITNTALRE DFGYKDFIWV FSGRRGAHCW VSDKRARALT
       190        200        210        220        230        240
DVQRRNVLDY VNVIRDRNTD KRLALKRPYH PHLARSLEQL KPFFVSIMLE EQNPWEDDQH
       250        260        270        280        290        300
AIQTLLPALY DKQLIDSLKK YWLDNPRRSS KEKWNDIDQI ATSLFKGPKQ DSHIIKLREC
       310        320        330        340        350        360
KEDLVLMTLY PKLDVEVTKQ TIHLLKAPFC IHPATGNVCV PIDESFAPEK APKLIDLQTE
       370        380        390        400
MEKNNDVSLT ALQPFINQFQ AYVSSLLKNE LGSVKRERED DDEPASLDF 

Encoded by PRI1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgaccaatt cagtaaagac taatgggcca agctcctcag acatggagta ctactataag
        70         80         90        100        110        120
tcgctctatc cattcaaaca cattttcaac tggctgaatc attcgcccaa accttctagg
       130        140        150        160        170        180
gatatgatta acagagaatt cgctatggcg ttcaggtcag gtgcttacaa aaggtataat
       190        200        210        220        230        240
tcttttaact ctgtgcaaga tttcaaagca caaatagaaa aagccaaccc agacagattt
       250        260        270        280        290        300
gaaatcggtg ccatatataa caaaccgcca agagaacgtg atactctatt aaaaagcgaa
       310        320        330        340        350        360
ttgaaagctt tagagaagga gttggtgttt gatattgata tggatgatta cgatgccttc
       370        380        390        400        410        420
agaacgtgtt gctccggagc ccaagtttgc tctaagtgct ggaaatttat atctttggca
       430        440        450        460        470        480
atgaaaatta cgaacacggc cctaagagag gattttggtt ataaggactt catctgggta
       490        500        510        520        530        540
ttttcaggta gacgtggtgc tcattgttgg gtaagcgaca aacgggcacg ggctttgacg
       550        560        570        580        590        600
gatgtacaac gaagaaacgt tttagattat gtcaacgtga ttagagacag aaatactgac
       610        620        630        640        650        660
aagcgattgg ctttgaagag gccttatcac cctcatcttg ctcgttcttt ggagcaattg
       670        680        690        700        710        720
aaacctttct ttgtcagcat tatgctcgaa gagcaaaatc cttgggaaga tgaccaacat
       730        740        750        760        770        780
gctatccaaa ctttattgcc tgcattatat gataaacaat tgattgattc attaaagaag
       790        800        810        820        830        840
tattggctgg acaatccaag gaggtcaagc aaagagaagt ggaatgatat agatcagata
       850        860        870        880        890        900
gctacatcgc tcttcaaagg ccccaagcaa gactctcaca taattaagtt acgtgaatgt
       910        920        930        940        950        960
aaggaagatc tcgtattgat gactctttat ccgaagctgg atgtggaagt tacaaagcaa
       970        980        990       1000       1010       1020
acaattcatt tgttaaaggc ccctttttgt attcatcctg ctacggggaa tgtctgtgtg
      1030       1040       1050       1060       1070       1080
cctattgatg aatcctttgc acctgaaaaa gcacctaagc taattgatct tcaaacagaa
      1090       1100       1110       1120       1130       1140
atggagaaaa ataatgatgt ttcattaaca gctttacaac cttttatcaa tcagttccaa
      1150       1160       1170       1180       1190       1200
gcatatgtga gttctctttt gaaaaatgaa ctgggttcag tgaaaagaga acgtgaagat
      1210       1220       1230
gatgatgaac cggcttcttt agatttctga   

Last modification date: Oct. 2, 2011