Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA polymerase alpha subunit B


DESCRIPTION:
Non-catalytic component of DNA polymerase alpha, which in a complex with DNA primase (DNA polymerase alpha:primase) constitutes a replicative polymerase. POL12 may play an essential role at the early stage of chromosomal DNA replication by coupling DNA polymerase alpha to the cellular replication machinery (By similarity). Interacts with MCM10.

MISCELLANEOUS:
Present with 11200 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the DNA polymerase alpha subunit B family.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated in a cell cycle-dependent manner.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSGSIDVITH FGPDADKPEI ITALENLTKL HALSVEDLYI KWEQFSNQRR QTHTDLTSKN
        70         80         90        100        110        120
IDEFKQFLQL QMEKRANQIS SSSKVNTSTK KPVIKKSLNS SPLFGLSIPK TPTLKKRKLH
       130        140        150        160        170        180
GPFSLSDSKQ TYNVGSEAET NEKGNSSLKL EFTPGMAEDA VGDSAPLSHA KSSDAKTPGS
       190        200        210        220        230        240
STFQTPTTNT PTTSRQNVPA GEILDSLNPE NIEISSGNPN VGLLSTEEPS YNQVKVEPFY
       250        260        270        280        290        300
DAKKYKFRTM RQNLQEASDV LDDQIESFTK IIQNHYKLSP NDFADPTIQS QSEIYAVGRI
       310        320        330        340        350        360
VPDSPTYDKF LNPESLSLET SRMGGVGRRV RLDLSQVNEL SFFLGQIVAF KGKNANGDYF
       370        380        390        400        410        420
TVNSILPLPY PNSPVSTSQE LQEFQANLEG SSLKVIVTCG PYFANDNFSL ELLQEFIDSI
       430        440        450        460        470        480
NNEVKPHVLI MFGPFIDITH PLIASGKLPN FPQFKTQPKT LDELFLKLFT PILKTISPHI
       490        500        510        520        530        540
QTVLIPSTKD AISNHAAYPQ ASLIRKALQL PKRNFKCMAN PSSFQINEIY FGCSNVDTFK
       550        560        570        580        590        600
DLKEVIKGGT TSSRYRLDRV SEHILQQRRY YPIFPGSIRT RIKPKDVSTK KETNDMESKE
       610        620        630        640        650        660
EKVYEHISGA DLDVSYLGLT EFVGGFSPDI MIIPSELQHF ARVVQNVVVI NPGRFIRATG
       670        680        690        700
NRGSYAQITV QCPDLEDGKL TLVEGEEPVY LHNVWKRARV DLIAS 

Encoded by POL12 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgagcggat ctatcgatgt catcactcat tttgggcctg atgcagacaa gccggaaatc
        70         80         90        100        110        120
atcactgcat tagagaattt aactaaactg catgcattgt ccgtcgagga tctatacatt
       130        140        150        160        170        180
aaatgggagc agttctccaa tcaaaggcgt caaactcaca cagatctgac ttccaagaac
       190        200        210        220        230        240
atagatgagt ttaaacaatt tttacaatta caaatggaaa agcgtgctaa ccaaatttca
       250        260        270        280        290        300
tcgtcctcta aggtaaacac atccacaaaa aaaccagtga ttaaaaaaag tttgaattca
       310        320        330        340        350        360
agtcccttgt ttggccttag tattccaaag actccgactt taaaaaaaag aaagttacat
       370        380        390        400        410        420
ggtccctttt cactcagtga ttccaagcaa acatataatg taggctctga ggcagaaaca
       430        440        450        460        470        480
aatgaaaaag gaaatagttc tttaaaattg gagtttactc caggcatggc agaagatgct
       490        500        510        520        530        540
gtgggtgaca gtgctccatt gtcccacgct aaaagctcgg atgccaagac tcccggctcg
       550        560        570        580        590        600
tcgacattcc aaactccaac aacaaacact cctaccacat cgaggcaaaa tgttcctgct
       610        620        630        640        650        660
ggtgaaattt tggattcttt gaaccctgaa aatattgaaa tatcatctgg taatccaaat
       670        680        690        700        710        720
gtaggccttc tttcgactga agaaccatca tataaccagg tcaaagtaga accattttat
       730        740        750        760        770        780
gatgctaaaa agtataaatt cagaacaatg aggcaaaact tacaggaagc gtctgatgtc
       790        800        810        820        830        840
ctcgatgatc aaattgaatc gtttactaag attattcaaa atcactataa gttatctcca
       850        860        870        880        890        900
aacgacttcg ctgatccaac tattcaatct cagtccgaaa tttatgctgt tggaaggata
       910        920        930        940        950        960
gttccagatt cgccgaccta cgacaagttt ttgaatccag aatcgctttc cctagagacc
       970        980        990       1000       1010       1020
tcaagaatgg gcggcgttgg aagaagggta aggttagatc tatcgcaggt caacgagctt
      1030       1040       1050       1060       1070       1080
tcgtttttct taggccaaat tgtggcattc aaaggtaaaa atgctaatgg ggattacttc
      1090       1100       1110       1120       1130       1140
accgttaact ctattttgcc gctaccttat ccaaactccc cagtgtccac atcgcaggaa
      1150       1160       1170       1180       1190       1200
ctgcaggagt ttcaagcaaa tttggaaggt tcttctttga aggttatcgt gacctgtggc
      1210       1220       1230       1240       1250       1260
ccatactttg caaatgataa cttctccctc gaattgttgc aagaatttat cgatagtata
      1270       1280       1290       1300       1310       1320
aataatgagg taaaacctca cgtcttgata atgtttggcc catttataga tatcacccat
      1330       1340       1350       1360       1370       1380
cctctgatag cgagtggtaa attgccaaat tttccccagt tcaagacaca gcccaaaaca
      1390       1400       1410       1420       1430       1440
ttggacgagc tttttttgaa gttatttact cctattctaa aaaccatatc accacatatc
      1450       1460       1470       1480       1490       1500
caaaccgtat tgataccctc cacaaaagat gcaatttcta atcacgccgc atatccacaa
      1510       1520       1530       1540       1550       1560
gcttctttaa ttagaaaggc tttacagctg cctaaaagga acttcaaatg catggctaat
      1570       1580       1590       1600       1610       1620
ccttcatctt ttcaaataaa tgagatatac ttcggttgct caaacgttga tacattcaaa
      1630       1640       1650       1660       1670       1680
gatctaaagg aagtcataaa aggcggtact acctcatcaa gatatagatt agatcgtgtt
      1690       1700       1710       1720       1730       1740
tctgagcata ttttacaaca gcgcaggtat taccccatat ttccaggtag tattcgtaca
      1750       1760       1770       1780       1790       1800
agaataaaac caaaagacgt atctacgaag aaggaaacca atgatatgga aagcaaagaa
      1810       1820       1830       1840       1850       1860
gaaaaagttt atgaacatat atctggtgca gatttagacg tgagttatct gggactaaca
      1870       1880       1890       1900       1910       1920
gagtttgttg gtgggttctc gcctgacata atgattatac ccagtgaatt acaacacttt
      1930       1940       1950       1960       1970       1980
gcaagagttg tccaaaacgt agttgttata aatcccggaa gatttattag agcaacaggt
      1990       2000       2010       2020       2030       2040
aacaggggat cctatgcaca aattactgtc caatgccctg atcttgaaga cgggaaattg
      2050       2060       2070       2080       2090       2100
acgcttgttg aaggtgaaga gccagtttat ctacacaacg tgtggaagcg cgctagagtt
      2110
gacttgattg ctagttga    

Last modification date: Oct. 2, 2011