Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA topoisomerase 3


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. Essential for proper chromosome segregation in both meiosis and mitosis. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA. The TOP3-SGS1 protein complex may function as a eukaryotic reverse gyrase introducing positive supercoils into extrachromosomal ribosomal DNA rings.

MISCELLANEOUS:
Present with 468 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 Toprim domain.
Belongs to the prokaryotic type I/III topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


PROTEIN TYPE(S):
endonuclease
DNA topoisomerase


RELATED PATHWAY(S):
DNA replication
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MKVLCVAEKN SIAKAVSQIL GGGRSTSRDS GYMYVKNYDF MFSGFPFARN GANCEVTMTS
        70         80         90        100        110        120
VAGHLTGIDF SHDSHGWGKC AIQELFDAPL NEIMNNNQKK IASNIKREAR NADYLMIWTD
       130        140        150        160        170        180
CDREGEYIGW EIWQEAKRGN RLIQNDQVYR AVFSHLERQH ILNAARNPSR LDMKSVHAVG
       190        200        210        220        230        240
TRIEIDLRAG VTFTRLLTET LRNKLRNQAT MTKDGAKHRG GNKNDSQVVS YGTCQFPTLG
       250        260        270        280        290        300
FVVDRFERIR NFVPEEFWYI QLVVENKDNG GTTTFQWDRG HLFDRLSVLT FYETCIETAG
       310        320        330        340        350        360
NVAQVVDLKS KPTTKYRPLP LTTVELQKNC ARYLRLNAKQ SLDAAEKLYQ KGFISYPRTE
       370        380        390        400        410        420
TDTFPHAMDL KSLVEKQAQL DQLAAGGRTA WASYAASLLQ PENTSNNNKF KFPRSGSHDD
       430        440        450        460        470        480
KAHPPIHPIV SLGPEANVSP VERRVYEYVA RHFLACCSED AKGQSMTLVL DWAVERFSAS
       490        500        510        520        530        540
GLVVLERNFL DVYPWARWET TKQLPRLEMN ALVDIAKAEM KAGTTAPPKP MTESELILLM
       550        560        570        580        590        600
DTNGIGTDAT IAEHIDKIQV RNYVRSEKVG KETYLQPTTL GVSLVHGFEA IGLEDSFAKP
       610        620        630        640        650
FQRREMEQDL KKICEGHASK TDVVKDIVEK YRKYWHKTNA CKNTLLQVYD RVKASM

Encoded by TOP3 gene


OTHER NAME(S):
EDR1


Nucleic acid sequence

        10         20         30         40         50         60
atgaaagtgc tatgtgtcgc agagaaaaat tctatagcga aggcagtttc acagatccta
        70         80         90        100        110        120
ggaggaggca gatcaacttc aagggattcc ggctacatgt atgtaaagaa ctatgatttc
       130        140        150        160        170        180
atgtttagtg ggttcccgtt tgccagaaat ggggctaact gcgaagttac catgactagt
       190        200        210        220        230        240
gttgcagggc acctaacagg cattgatttc agccatgatt cgcatgggtg gggaaaatgc
       250        260        270        280        290        300
gccatccaag agttatttga tgcgccactg aacgagatta tgaataacaa ccaaaaaaag
       310        320        330        340        350        360
atagcaagca acatcaagcg agaagcgagg aatgcagact atctgatgat atggacagat
       370        380        390        400        410        420
tgcgaccggg aaggagagta catcggttgg gagatatggc aggaggccaa gagaggcaac
       430        440        450        460        470        480
aggctcatac aaaatgatca agtataccgg gcagtctttt cgcatctcga aagacaacac
       490        500        510        520        530        540
atattaaatg cagcacgaaa cccaagtcga ttggatatga agagtgtgca cgctgtaggc
       550        560        570        580        590        600
acgcggattg aaatcgatct tcgagcaggt gttacattca ccagactctt aacagaaacg
       610        620        630        640        650        660
ctacgaaata aactgagaaa ccaagccacc atgaccaagg atggtgcaaa acaccgcggt
       670        680        690        700        710        720
ggtaacaaga acgactcaca agtcgtatcg tatggtacat gccagtttcc aacgctcggc
       730        740        750        760        770        780
tttgtagtag acaggtttga aagaatacga aattttgttc ccgaagagtt ctggtatatc
       790        800        810        820        830        840
caattggtag tcgaaaacaa agacaacggc ggaacaacaa cgttccagtg ggacaggggc
       850        860        870        880        890        900
cacttgttcg accggctgag cgtgttaacg ttttacgaga catgcatcga aaccgccggc
       910        920        930        940        950        960
aatgttgctc aagtagtaga cttgaaatca aagccaacaa cgaaatacag acctttacct
       970        980        990       1000       1010       1020
ctgaccacag tggagctaca aaaaaactgc gcccggtacc tgcgtctgaa cgccaaacaa
      1030       1040       1050       1060       1070       1080
tcactagacg cagcagaaaa gctataccaa aaggggttca tatcgtatcc aagaacagag
      1090       1100       1110       1120       1130       1140
actgatactt tcccacacgc aatggaccta aaatccttgg tcgaaaagca agctcaattg
      1150       1160       1170       1180       1190       1200
gaccaactcg ctgcaggcgg cagaaccgcc tgggcatcgt acgcggcatc gctgctccaa
      1210       1220       1230       1240       1250       1260
cccgaaaaca caagtaacaa taacaagttc aagtttccac gaagcggctc ccatgacgac
      1270       1280       1290       1300       1310       1320
aaagcgcatc caccaatcca ccccatcgta agtctggggc ctgaagcaaa tgtttcgcca
      1330       1340       1350       1360       1370       1380
gtggaaagaa gagtatacga gtacgtggcc aggcactttt tggcatgctg ctcagaggac
      1390       1400       1410       1420       1430       1440
gccaagggcc aatcgatgac ccttgtgttg gactgggccg ttgaacgttt ctcagcttca
      1450       1460       1470       1480       1490       1500
ggtctcgtag tcctagagag aaatttcctc gatgtttacc cttgggcccg atgggaaacc
      1510       1520       1530       1540       1550       1560
accaagcagt taccgcggct tgaaatgaat gccctcgtag acatcgcgaa ggccgaaatg
      1570       1580       1590       1600       1610       1620
aaggcgggca ctacggcgcc gcccaagccg atgactgaga gtgaactcat tctcctcatg
      1630       1640       1650       1660       1670       1680
gatacaaacg gcattggcac agacgccacc attgcggagc acatagacaa gatccaagta
      1690       1700       1710       1720       1730       1740
cgtaattacg ttaggagcga gaaagtaggc aaggaaacct acttacaacc cacgaccctg
      1750       1760       1770       1780       1790       1800
ggtgtctcac tagtgcacgg cttcgaggcc atcggcctcg aagactcctt tgcaaagccc
      1810       1820       1830       1840       1850       1860
ttccagcgca gagaaatgga gcaagacctc aagaaaatct gcgaaggtca tgcctccaag
      1870       1880       1890       1900       1910       1920
actgatgttg taaaggacat agtcgagaag tataggaagt actggcacaa gacgaatgcc
      1930       1940       1950       1960       1970
tgcaagaata ctctcttgca agtttatgac cgtgtcaagg catccatgta a

Last modification date: Oct. 2, 2011