Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Ribonuclease H


DESCRIPTION:
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.

MISCELLANEOUS:
Present with 149 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 RNase H domain.
Belongs to the RNase H family.


CATALYTIC ACTIVITY:
Endonucleolytic cleavage to 5'-phosphomonoester.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MARQGNFYAV RKGRETGIYN TWNECKNQVD GYGGAIYKKF NSYEQAKSFL GQPNTTSNYG
        70         80         90        100        110        120
SSTHAGGQVS KPHTTQKRVH RRNRPLHYSS LTSSSACSSL SSANTNTFYS VKSNVPNIES
       130        140        150        160        170        180
KIFNNWKDCQ AYVKHKRGIT FKKFEDQLAA ENFISGMSAH DYKLMNISKE SFESKYKLSS
       190        200        210        220        230        240
NTMYNKSMNV YCDGSSFGNG TSSSRAGYGA YFEGAPEENI SEPLLSGAQT NNRAEIEAVS
       250        260        270        280        290        300
EALKKIWEKL TNEKEKVNYQ IKTDSEYVTK LLNDRYMTYD NKKLEGLPNS DLIVPLVQRF
       310        320        330        340
VKVKKYYELN KECFKNNGKF QIEWVKGHDG DPGNEMADFL AKKGASRR 

Encoded by RNH1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atggcaaggc aagggaactt ctacgcggtt agaaagggca gggaaactgg gatctataat
        70         80         90        100        110        120
acatggaatg aatgtaaaaa ccaagttgat ggttatggtg gtgcaattta taaaaaattt
       130        140        150        160        170        180
aacagctacg agcaagcgaa atctttccta ggtcaaccaa atactacatc aaactatgga
       190        200        210        220        230        240
agttcaacgc atgctggagg gcaagttagt aaacctcata ccactcaaaa gcgagttcat
       250        260        270        280        290        300
cgaaggaatc ggccacttca ttattcctcc ttgacatcgt catcagcatg ttcatcttta
       310        320        330        340        350        360
agctctgcta atacaaatac cttttattca gtaaagagta atgttccaaa tatcgagagt
       370        380        390        400        410        420
aaaatcttta acaattggaa agactgtcaa gcctatgtca agcataaaag gggtataacc
       430        440        450        460        470        480
ttcaagaagt ttgaagacca attagctgcg gaaaacttca ttagtggtat gagtgcgcat
       490        500        510        520        530        540
gattacaagc tcatgaatat atccaaagaa agttttgaat ctaagtataa gctttctagc
       550        560        570        580        590        600
aataccatgt acaacaagtc tatgaacgtt tactgtgatg gttcaagttt tggaaacggc
       610        620        630        640        650        660
acgtcatctt caagggcagg ttatggtgca tattttgaag gagcgccaga ggaaaatatt
       670        680        690        700        710        720
tcagaaccct tattatcggg agctcaaact aataatagag ctgaaatcga agcagtatca
       730        740        750        760        770        780
gaagctttga agaaaatctg ggaaaagtta actaatgaaa aggaaaaagt aaattatcaa
       790        800        810        820        830        840
atcaaaactg attcagagta cgtaaccaaa ttattaaatg acaggtatat gacgtatgat
       850        860        870        880        890        900
aataaaaaac ttgaagggct acctaattct gatttaattg ttcctttggt gcagaggttc
       910        920        930        940        950        960
gttaaagtta aaaagtacta tgagttgaat aaagagtgct tcaaaaataa tggtaaattt
       970        980        990       1000       1010       1020
cagattgaat gggtaaaagg acacgatggt gatccaggaa atgaaatggc agattttctg
      1030       1040
gcgaagaaag gagcatctag acgataa   

Last modification date: Oct. 2, 2011