Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA ligase B


DESCRIPTION:
Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction.

MISCELLANEOUS:
Considerably less active in nick joining than ligA.


STRUCTURE SIMILARITY:
Belongs to the NAD-dependent DNA ligase family. LigB subfamily.


CATALYTIC ACTIVITY:
NAD(+) + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m).


PROTEIN TYPE(S):
DNA ligase


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MKVWMAILIG ILCWQSSVWA VCPAWSPARA QEEISRLQQQ IKQWDDDYWK EGKSEVEDGV
        70         80         90        100        110        120
YDQLSARLTQ WQRCFGSEPR DVMMPPLNGA VMHPVAHTGV RKMVDKNALS LWMRERSDLW
       130        140        150        160        170        180
VQPKVDGVAV TLVYRDGKLN KAISRGNGLK GEDWTQKVSL ISAVPQTVSG PLANSTLQGE
       190        200        210        220        230        240
IFLQREGHIQ QQMGGINARA KVAGLMMRQD DSDTLNSLGV FVWAWPDGPQ LMSDRLKELA
       250        260        270        280        290        300
TAGFTLTQTY TRAVKNADEV ARVRNEWWKA ELPFVTDGVV VRAAKEPESR HWLPGQAEWL
       310        320        330        340        350        360
VAWKYQPVAQ VAEVKAIQFA VGKSGKISVV ASLAPVMLDD KKVQRVNIGS VRRWQEWDIA
       370        380        390        400        410        420
PGDQILVSLA GQGIPRIDDV VWRGAERTKP TPPENRFNSL TCYFASDVCQ EQFISRLVWL
       430        440        450        460        470        480
GAKQVLGLDG IGEAGWRALH QTHRFEHIFS WLLLTPEQLQ NTPGIAKSKS AQLWHQFNLA
       490        500        510        520        530        540
RKQPFTRWVM AMGIPLTRAA LNASDERSWS QLLFSTEQFW QQLPGTGSGR ARQVIEWKEN
       550        560
AQIKKLGSWL AAQQITGFEP    

Encoded by ligB gene


OTHER NAME(S):
ECK3637
JW3622
yicF


DESCRIPTION:
LigB is a NAD(+)-dependent DNA ligase . [More information is available at EcoCyc: EG11334].


Nucleic acid sequence

        10         20         30         40         50         60
atgaaagtat ggatggcgat attaataggt atcttgtgct ggcaatcatc tgtgtgggcg
        70         80         90        100        110        120
gtctgtccgg cctggtcgcc agccagagca caggaagaaa tttcccgcct gcaacagcaa
       130        140        150        160        170        180
ataaaacagt gggacgatga ctactggaag gaaggaaaaa gtgaggtgga agacggtgtt
       190        200        210        220        230        240
tacgatcagt taagcgcccg tcttacgcag tggcaacgct gctttgggag cgagccccgc
       250        260        270        280        290        300
gacgtgatga tgccgccttt aaatggtgcg gttatgcatc cggttgccca tacgggtgta
       310        320        330        340        350        360
cgcaaaatgg tggataaaaa cgcattaagt ctgtggatgc gagaacgtag cgatctttgg
       370        380        390        400        410        420
gtgcagccaa aagttgatgg cgttgcggta accctggttt atcgggacgg gaaactgaac
       430        440        450        460        470        480
aaagcaatca gtcgcggtaa tggcctgaaa ggcgaggact ggacgcagaa agttagctta
       490        500        510        520        530        540
atttccgctg tgccgcaaac cgttagcggg cctttagcca acagtacgct tcagggggaa
       550        560        570        580        590        600
atatttctcc agcgcgaggg gcatatccaa caacaaatgg ggggaataaa tgcccgcgca
       610        620        630        640        650        660
aaagttgctg gcttgatgat gcgccaggac gatagcgaca cgctgaattc tctgggcgtt
       670        680        690        700        710        720
tttgtctggg catggccgga tggaccgcag ttaatgtctg atcgtttaaa agagctggct
       730        740        750        760        770        780
accgcaggtt ttactctaac gcagacgtat acccgtgcgg tgaaaaatgc tgatgaagtt
       790        800        810        820        830        840
gcgcgcgtac gcaatgagtg gtggaaagcg gaattaccct tcgtcaccga tggcgtagtt
       850        860        870        880        890        900
gtacgagcgg cgaaagagcc agaatcccgc cattggctac cgggccaggc agagtggctg
       910        920        930        940        950        960
gtggcctgga aatatcaacc tgtagctcag gttgccgaag tgaaggcaat tcagtttgcg
       970        980        990       1000       1010       1020
gtgggtaaga gcggtaaaat atcggtggtt gcgtcactcg cacctgtcat gctggatgat
      1030       1040       1050       1060       1070       1080
aaaaaagtcc agcgggtgaa tattggttcc gtcaggcgct ggcaggagtg ggatattgcg
      1090       1100       1110       1120       1130       1140
cctggtgatc agattctcgt cagccttgcc ggtcagggta ttcctcgcat tgatgatgtg
      1150       1160       1170       1180       1190       1200
gtgtggcgcg gtgcagaacg tacaaaaccg acaccgccag aaaaccgctt taactcgttg
      1210       1220       1230       1240       1250       1260
acctgctact ttgcttctga tgtttgtcag gaacagttca tttcacgctt agtctggctg
      1270       1280       1290       1300       1310       1320
ggggcaaaac aggttcttgg gctggatggc attggtgagg ccggttggcg cgcgctgcat
      1330       1340       1350       1360       1370       1380
cagactcatc gctttgaaca tatcttttcc tggcttttat taacgccaga gcaattacag
      1390       1400       1410       1420       1430       1440
aacacgccgg ggatcgcgaa aagtaaaagt gcgcagctat ggcatcagtt taatctggct
      1450       1460       1470       1480       1490       1500
cgtaagcagc cttttactcg ctgggtgatg gcaatgggaa taccgctaac ccgggcggcg
      1510       1520       1530       1540       1550       1560
cttaatgcca gtgatgaacg gtcctggtcg caacttttat ttagcacgga gcagttctgg
      1570       1580       1590       1600       1610       1620
cagcagctgc cggggactgg atcgggacgc gccagacagg ttattgaatg gaaggaaaat
      1630       1640       1650       1660       1670       1680
gcgcaaatca agaagctggg cagttggctg gctgcccagc agatcacagg ttttgaacct

tag     

Last modification date: Oct. 2, 2011