Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA ligase


DESCRIPTION:
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

STRUCTURE SIMILARITY:
Belongs to the NAD-dependent DNA ligase family. LigA subfamily.
Contains 1 BRCT domain.


CATALYTIC ACTIVITY:
NAD(+) + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m).


PROTEIN TYPE(S):
DNA ligase


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MESIEQQLTE LRTTLRHHEY LYHVMDAPEI PDAEYDRLMR ELRELETKHP ELITPDSPTQ
        70         80         90        100        110        120
RVGAAPLAAF SQIRHEVPML SLDNVFDEES FLAFNKRVQD RLKNNEKVTW CCELKLDGLA
       130        140        150        160        170        180
VSILYENGVL VSAATRGDGT TGEDITSNVR TIRAIPLKLH GENIPARLEV RGEVFLPQAG
       190        200        210        220        230        240
FEKINEDARR TGGKVFANPR NAAAGSLRQL DPRITAKRPL TFFCYGVGVL EGGELPDTHL
       250        260        270        280        290        300
GRLLQFKKWG LPVSDRVTLC ESAEEVLAFY HKVEEDRPTL GFDIDGVVIK VNSLAQQEQL
       310        320        330        340        350        360
GFVARAPRWA VAFKFPAQEQ MTFVRDVEFQ VGRTGAITPV ARLEPVHVAG VLVSNATLHN
       370        380        390        400        410        420
ADEIERLGLR IGDKVVIRRA GDVIPQVVNV VLSERPEDTR EVVFPTHCPV CGSDVERVEG
       430        440        450        460        470        480
EAVARCTGGL ICGAQRKESL KHFVSRRAMD VDGMGDKIID QLVEKEYVHT PADLFKLTAG
       490        500        510        520        530        540
KLTGLERMGP KSAQNVVNAL EKAKETTFAR FLYALGIREV GEATAAGLAA YFGTLEALEA
       550        560        570        580        590        600
ASIEELQKVP DVGIVVASHV HNFFAEESNR NVISELLAEG VHWPAPIVIN AEEIDSPFAG
       610        620        630        640        650        660
KTVVLTGSLS QMSRDDAKAR LVELGAKVAG SVSKKTDLVI AGEAAGSKLA KAQELGIEVI
       670
DEAEMLRLLG S    

Encoded by ligA gene


OTHER NAME(S):
dnaL
ECK2406
JW2403
lig
lop
pdeC


DESCRIPTION:
ligA is an essential gene. [More information is available at EcoGene: EG10534]. LigA is one of two known NAD(+)-dependent DNA ligases, catalyzing the formation of phosphodiester bonds in duplex DNA. [More information is available at EcoCyc: EG10534].


Nucleic acid sequence

        10         20         30         40         50         60
atggaatcaa tcgaacaaca actgacagaa ctgcgaacga cgcttcgcca tcatgaatat
        70         80         90        100        110        120
ctttatcatg tgatggatgc gccggaaatt cccgacgctg aatacgacag gctgatgcgc
       130        140        150        160        170        180
gaactgcgcg agctggaaac caaacatcca gaactgatta cgcctgattc gcctactcaa
       190        200        210        220        230        240
cgtgtaggcg ctgcgccgct ggcggctttc agccagatac gccatgaagt accaatgctg
       250        260        270        280        290        300
tcactggata acgtttttga tgaagaaagc tttcttgctt tcaacaaacg tgtgcaggac
       310        320        330        340        350        360
cgtctgaaaa acaacgagaa agtcacctgg tgctgtgagc tgaagctgga tggtcttgcc
       370        380        390        400        410        420
gtcagtattc tgtatgaaaa tggcgtttta gtcagtgccg cgacccgtgg cgatggcacc
       430        440        450        460        470        480
accggggaag atatcacgtc taatgtgcgt actattcgcg ccattccgct gaagctgcac
       490        500        510        520        530        540
ggagagaata tcccggcgcg tctggaagtg cgtggtgaag tgttcctgcc gcaggcgggg
       550        560        570        580        590        600
ttcgaaaaga ttaacgaaga tgcgcgacgc acgggcggga aagtgtttgc taacccacgt
       610        620        630        640        650        660
aatgcggcag ctggttcact gcgtcagctt gatccgcgta ttacagcgaa gcgaccgctc
       670        680        690        700        710        720
acttttttct gctatggcgt tggtgttctg gaaggtggcg agctgccgga tactcatctt
       730        740        750        760        770        780
ggccgtttac tgcaatttaa aaagtggggg ttgccggtca gcgatcgggt aacgctttgt
       790        800        810        820        830        840
gaatcggcgg aagaagtgct ggcgttctat cacaaagtgg aagaagaccg cccgacgctg
       850        860        870        880        890        900
ggctttgata tcgacggcgt ggtgattaag gtcaactcac tggcacagca ggagcagctt
       910        920        930        940        950        960
ggctttgtcg cgcgtgcccc gcgctgggcg gtagcgttta aattcccggc gcaggagcag
       970        980        990       1000       1010       1020
atgacctttg tgcgtgacgt cgagtttcag gttgggcgta ctggcgcgat tacgcctgtt
      1030       1040       1050       1060       1070       1080
gcgcgtctgg aacctgtcca tgttgcaggc gtgctggtga gtaacgcaac cttacacaat
      1090       1100       1110       1120       1130       1140
gcggatgaaa tcgaacgtct tggtttacgc attggcgata aagtggtgat tcgccgcgct
      1150       1160       1170       1180       1190       1200
ggcgacgtga tcccgcaggt ggttaacgtc gtgctttctg aacgcccgga agatacccgt
      1210       1220       1230       1240       1250       1260
gaggttgtat tcccgacgca ttgtccggta tgtggttctg acgttgagcg tgtggaaggt
      1270       1280       1290       1300       1310       1320
gaagcggttg cccgctgtac cggtggcctg atttgcggtg cgcagcgtaa agagtcgctg
      1330       1340       1350       1360       1370       1380
aaacactttg tttcccgccg tgcgatggat gttgacggaa tgggcgacaa aatcatcgat
      1390       1400       1410       1420       1430       1440
cagctggttg aaaaagaata tgtccatact ccggcagatc tgttcaaact caccgcaggc
      1450       1460       1470       1480       1490       1500
aaactgaccg gactggagcg tatggggcca aaatcggcac aaaacgtggt taacgcgctg
      1510       1520       1530       1540       1550       1560
gaaaaagcga aagaaaccac ctttgctcgc ttcctctatg cacttggcat ccgtgaagtc
      1570       1580       1590       1600       1610       1620
ggcgaggcca ccgcagcagg tctggcggca tatttcggca cgctggaagc gctggaagcc
      1630       1640       1650       1660       1670       1680
gcttcgattg aagagctgca aaaggtgcct gatgttggca ttgtcgttgc atcccacgtt
      1690       1700       1710       1720       1730       1740
cacaacttct ttgccgaaga aagcaaccgc aatgtcatca gcgagctgtt ggcggaaggt
      1750       1760       1770       1780       1790       1800
gttcactggc ctgcgccgat cgttatcaac gcggaagaga ttgacagccc gtttgctggt
      1810       1820       1830       1840       1850       1860
aaaaccgtgg tgcttacggg cagcttaagc cagatgtcgc gtgatgacgc taaagctcga
      1870       1880       1890       1900       1910       1920
ctggtcgaac tgggcgcgaa agtcgcgggc agcgtgtcga agaaaaccga tctggtgata
      1930       1940       1950       1960       1970       1980
gcgggtgaag ctgcaggatc taaactggcg aaggcgcagg aactgggcat tgaagtcatc
      1990       2000       2010
gacgaagcgg aaatgctgcg tttgctgggt agctga  

Last modification date: Oct. 2, 2011