Homo sapiens L. (human) [HSA]

FULL NAME: Crossover junction endonuclease EME1


DESCRIPTION:
Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.

STRUCTURE SIMILARITY:
Belongs to the EME1/MMS4 family.


PROTEIN TYPE(S):
recombinase
DNase
endonuclease


RELATED PATHWAY(S):
Fanconi anemia (FA) pathway
homologous recombination (HR)


RELATED DAMAGE:
intErstrand crosslinks (ICLs)


Amino acids sequence

        10         20         30         40         50         60
MALKKSSPSL DSGDSDSEEL PTFAFLKKEP SSTKRRQPER EEKIVVVDIS DCEASCPPAP
        70         80         90        100        110        120
ELFSPPVPEI AETVTQTQPV RLLSSESEDE EEFIPLAQRL TCKFLTHKQL SPEDSSSPVK
       130        140        150        160        170        180
SVLDHQNNEG ASCDWKKPFP KIPEVPLHDT PERSAADNKD LILDPCCQLP AYLSTCPGQS
       190        200        210        220        230        240
SSLAVTKTNS DILPPQKKTK PSQKVQGRGS HGCRQQRQAR QKESTLRRQE RKNAALVTRM
       250        260        270        280        290        300
KAQRPEECLK HIIVVLDPVL LQMEGGGQLL GALQTMECRC VIEAQAVPCS VTWRRRAGPS
       310        320        330        340        350        360
EDREDWVEEP TVLVLLRAEA FVSMIDNGKQ GSLDSTMKGK ETLQGFVTDI TAKTAGKALS
       370        380        390        400        410        420
LVIVDQEKCF SLELLFFDFL PCTSAQNPPR RGKQGANKQT KKQQQRQPEA SIGSMVSRVD
       430        440        450        460        470        480
AEEALVDLQL HTEAQAQIVQ SWKELADFTC AFTKAVAEAP FKKLRDETTF SFCLESDWAG
       490        500        510        520        530        540
GVKVDLAGRG LALVWRRQIQ QLNRVSLEMA SAVVNAYPSP QLLVQAYQQC FSDKERQNLL
       550        560        570        580
ADIQVRRGEG VTSTSRRIGP ELSRRIYLQM TTLQPHLSLD SAD 

Encoded by EME1 gene

FULL NAME: essential meiotic endonuclease 1 homolog 1 (S. pombe)


OTHER NAME(S):
FLJ31364
MMS4L
SLX2A


DESCRIPTION:
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009]


Nucleic acid sequence

        10         20         30         40         50         60
atggctctaa agaagtcatc accctcactg gattctggtg atagtgactc tgaggagttg
        70         80         90        100        110        120
ccaacatttg cctttctgaa gaaggaacca tcttcaacaa agaggagaca gcctgaaagg
       130        140        150        160        170        180
gaagagaaga ttgtagtggt tgacatctca gattgtgaag cctcctgtcc tccagcacca
       190        200        210        220        230        240
gagttatttt caccacctgt cccagaaata gctgaaactg tcacacaaac acagccagtc
       250        260        270        280        290        300
aggttgctaa gcagtgaaag tgaagatgaa gaagaattta ttcctctggc tcaaaggctt
       310        320        330        340        350        360
acatgtaagt ttctgaccca caagcaactg agccctgagg actctagctc cccagttaaa
       370        380        390        400        410        420
agtgttttgg atcatcaaaa taatgaaggt gcatcatgtg actggaaaaa gccctttcca
       430        440        450        460        470        480
aagatccctg aagttcccct ccatgatacc ccagagagga gtgcagcaga taacaaggac
       490        500        510        520        530        540
ctgatcttag atccatgctg tcagcttcca gcctacctgt ctacctgccc tggccagagc
       550        560        570        580        590        600
agcagcttgg cagtaaccaa aacaaattct gacatccttc caccccagaa gaaaaccaag
       610        620        630        640        650        660
ccgagtcaga aggtccaggg aagaggctca cacggatgcc ggcagcagag acaagcaagg
       670        680        690        700        710        720
cagaaggaaa gcaccctgag aagacaggaa agaaagaatg cagcactggt taccaggatg
       730        740        750        760        770        780
aaagcccaga ggccagagga atgcttaaaa cacatcattg tagtgctgga tccagtgctc
       790        800        810        820        830        840
ttacagatgg aaggtggggg ccagctccta ggagcactgc agaccatgga gtgccgctgt
       850        860        870        880        890        900
gtgattgagg cgcaggctgt gccttgcagt gtcacttgga ggagaagggc tgggccgtct
       910        920        930        940        950        960
gaggacagag aggactgggt ggaggagcca acagtactgg tgttgctccg ggcagaggca
       970        980        990       1000       1010       1020
tttgtgtcca tgatcgacaa tggaaagcag ggaagcctgg acagcactat gaaagggaag
      1030       1040       1050       1060       1070       1080
gaaacgcttc agggctttgt aactgacatc acagcaaaga cagcagggaa agctctgtca
      1090       1100       1110       1120       1130       1140
ctggtgattg tggatcagga gaaatgcttc agcctggagc tgctgttctt tgatttcctc
      1150       1160       1170       1180       1190       1200
ccctgcacca gtgctcagaa tcctccaaga agagggaaac agggagcaaa taaacagacc
      1210       1220       1230       1240       1250       1260
aagaagcagc agcagagaca accagaggcc agcatagggt ccatggtatc cagggtagac
      1270       1280       1290       1300       1310       1320
gctgaagagg cattggtgga tctgcagcta cacacagaag cccaggctca aattgtgcag
      1330       1340       1350       1360       1370       1380
agctggaaag agctggccga cttcacatgc gcattcacaa aggctgtggc tgaggcgccc
      1390       1400       1410       1420       1430       1440
ttcaagaagc tccgagatga aactaccttc tccttctgtc tggagagtga ctgggctgga
      1450       1460       1470       1480       1490       1500
ggggtgaagg tggaccttgc tggcagggga ctcgcactag tctggaggag acagattcag
      1510       1520       1530       1540       1550       1560
cagctgaacc gagtcagcct ggaaatggcc agtgcagttg tgaatgccta tccctcccca
      1570       1580       1590       1600       1610       1620
cagctcctgg tacaggctta tcagcagtgt ttttcggata aagaacgcca gaatttgctc
      1630       1640       1650       1660       1670       1680
gcagacatac aggtgcgccg tggggaaggt gtgacatcca cttctcgccg cattggacca
      1690       1700       1710       1720       1730       1740
gaactatcca ggcgtatcta ccttcagatg accactttac agccacatct ctctttagat
      1750
agtgctgact ga    

Last modification date: Oct. 10, 2011