Homo sapiens L. (human) [HSA]

FULL NAME: Crossover junction endonuclease MUS81


DESCRIPTION:
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. 3'-flap endonuclease activity

MISCELLANEOUS:
Expressed in S phase and G2 phase. Recruited to foci of DNA damage in S-phase cells. Up-regulated in cells treated with agents that damage DNA or block replication. This up-regulation seems to be independent of transcription.


STRUCTURE SIMILARITY:
Belongs to the XPF family. Contains 1 ERCC4 domain.


SUBUNIT STRUCTURE:
May self-associate. Interacts with EME1, EME2 and CHEK2. Interacts with BLM, and this interaction may stimulate the endonuclease activity of MUS81. Interacts with SLX4/BTBD12; this interaction is direct and links the MUS81-EME1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair. Interacts with DCLRE1B/Apollo.


PROTEIN TYPE(S):
DNase
endonuclease


RELATED PATHWAY(S):
Fanconi anemia (FA) pathway
homologous recombination (HR)


RELATED DAMAGE:
intErstrand crosslinks (ICLs)


Amino acids sequence

        10         20         30         40         50         60
MAAPVRLGRK RPLPACPNPL FVRWLTEWRD EATRSRRRTR FVFQKALRSL RRYPLPLRSG
        70         80         90        100        110        120
KEAKILQHFG DGLCRMLDER LQRHRTSGGD HAPDSPSGEN SPAPQGRLAE VQDSSMPVPA
       130        140        150        160        170        180
QPKAGGSGSY WPARHSGARV ILLVLYREHL NPNGHHFLTK EELLQRCAQK SPRVAPGSAR
       190        200        210        220        230        240
PWPALRSLLH RNLVLRTHQP ARYSLTPEGL ELAQKLAESE GLSLLNVGIG PKEPPGEETA
       250        260        270        280        290        300
VPGAASAELA SEAGVQQQPL ELRPGEYRVL LCVDIGETRG GGHRPELLRE LQRLHVTHTV
       310        320        330        340        350        360
RKLHVGDFVW VAQETNPRDP ANPGELVLDH IVERKRLDDL CSSIIDGRFR EQKFRLKRCG
       370        380        390        400        410        420
LERRVYLVEE HGSVHNLSLP ESTLLQAVTN TQVIDGFFVK RTADIKESAA YLALLTRGLQ
       430        440        450        460        470        480
RLYQGHTLRS RPWGTPGNPE SGAMTSPNPL CSLLTFSDFN AGAIKNKAQS VREVFARQLM
       490        500        510        520        530        540
QVRGVSGEKA AALVDRYSTP ASLLAAYDAC ATPKEQETLL STIKCGRLQR NLGPALSRTL
       550
SQLYCSYGPL T    

Encoded by MUS81 gene

FULL NAME: MUS81 endonuclease homolog (S. cerevisiae)


OTHER NAME(S):
FLJ21012
FLJ44872
SLX3


Nucleic acid sequence

        10         20         30         40         50         60
atggcggccc cggtccgcct gggccggaag cgcccgctgc ctgcctgtcc caacccgctc
        70         80         90        100        110        120
ttcgttcgct ggctgaccga gtggcgggac gaggcgaccc gcagcaggcg ccgcacgcgc
       130        140        150        160        170        180
ttcgtatttc agaaggcgct gcgttccctc cgacggtacc cactgccgct gcgcagcggg
       190        200        210        220        230        240
aaggaagcta agatcctaca gcacttcgga gacgggctct gccggatgct ggacgagcgg
       250        260        270        280        290        300
ctgcagcggc accgaacatc gggcggtgac catgccccgg actcaccatc tggagagaac
       310        320        330        340        350        360
agtccagccc cgcaggggcg acttgcggaa gtccaggact cttccatgcc agttcctgcc
       370        380        390        400        410        420
cagcccaaag cgggaggctc tggcagctac tggccagctc ggcactcagg agcccgagtg
       430        440        450        460        470        480
atactgctgg tgctctaccg ggagcacctg aatcctaatg gtcaccactt cttaaccaag
       490        500        510        520        530        540
gaggagctgc tgcagaggtg tgctcagaag tcccccaggg tagcccctgg gagtgctcga
       550        560        570        580        590        600
ccctggccag ccctccgctc cctccttcac aggaacctgg tcctcaggac acaccagcca
       610        620        630        640        650        660
gccaggtact cattgacccc agagggcctg gagctggccc agaagttggc cgagtcagaa
       670        680        690        700        710        720
ggcctgagct tgctgaatgt gggcatcggg cccaaggagc cccctgggga ggagacagca
       730        740        750        760        770        780
gtgccaggag cagcttcagc agagcttgcc agtgaagcag gggtccagca gcagccactg
       790        800        810        820        830        840
gagctgaggc ctggagagta cagggtgctg ttgtgtgtgg acattggcga gacccggggg
       850        860        870        880        890        900
ggcgggcaca ggccggagct gctccgagag ctacagcggc tgcacgtgac ccacacggtg
       910        920        930        940        950        960
cgcaagctgc acgttggaga ttttgtgtgg gtggcccagg agaccaatcc tagagaccca
       970        980        990       1000       1010       1020
gcaaaccctg gggagttggt actggatcac attgtggagc gcaagcgact ggatgacctt
      1030       1040       1050       1060       1070       1080
tgcagcagca tcatcgacgg ccgcttccgg gagcagaagt tccggctgaa gcgctgtggt
      1090       1100       1110       1120       1130       1140
ctggagcgcc gggtatacct ggtggaagag catggttccg tccacaacct cagccttcct
      1150       1160       1170       1180       1190       1200
gagagcacac tgctgcaggc tgtcaccaac actcaggtca ttgatggctt ttttgtgaag
      1210       1220       1230       1240       1250       1260
cgcacagcag acattaagga gtcagccgcc tacctggccc tcttgacgcg gggcctgcag
      1270       1280       1290       1300       1310       1320
agactctacc agggccacac cctacgcagc cgcccctggg gaacccctgg gaaccctgaa
      1330       1340       1350       1360       1370       1380
tcaggggcca tgacctctcc aaaccctctc tgctcactcc tcaccttcag tgacttcaac
      1390       1400       1410       1420       1430       1440
gcaggagcca tcaagaataa ggcccagtcg gtgcgagaag tgtttgcccg gcagctgatg
      1450       1460       1470       1480       1490       1500
caggtgcgcg gagtgagtgg ggagaaggca gcagccctgg tggatcgata cagcacccct
      1510       1520       1530       1540       1550       1560
gccagcctcc tggccgccta tgatgcctgt gccaccccca aggaacaaga gacactgctg
      1570       1580       1590       1600       1610       1620
agcaccatta agtgtgggcg tctacagagg aatctggggc ctgctctgag caggacctta
      1630       1640       1650
tcccagctct actgcagcta cggccccttg acctga  

Last modification date: Feb. 6, 2012