Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Ribonuclease HI


DESCRIPTION:
Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. RNase H participates in DNA replication; it helps to specify the origin of genomic replication by suppressing initiation at origins other than the oriC locus; along with the 5'-3' exonuclease of pol1, it removes RNA primers from the Okazaki fragments of lagging strand synthesis; and it defines the origin of replication for ColE1-type plasmids by specific cleavage of an RNA preprimer.

STRUCTURE SIMILARITY:
Contains 1 RNase H domain.
Belongs to the RNase H family.


CATALYTIC ACTIVITY:
Endonucleolytic cleavage to 5'-phosphomonoester.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MLKQVEIFTD GSCLGNPGPG GYGAILRYRG REKTFSAGYT RTTNNRMELM AAIVALEALK
        70         80         90        100        110        120
EHCEVILSTD SQYVRQGITQ WIHNWKKRGW KTADKKPVKN VDLWQRLDAA LGQHQIKWEW
       130        140        150
VKGHAGHPEN ERCDELARAA AMNPTLEDTG YQVEV  

Encoded by rnhA gene


OTHER NAME(S):
cer
dasF
ECK0214
herA
JW0204
rnh
sdrA
sin


DESCRIPTION:
RNase H cleaves RNA in RNA-DNA hybrids. [More information is available at EcoCyc: EG10860].


Nucleic acid sequence

        10         20         30         40         50         60
atgcttaaac aggtagaaat tttcaccgat ggttcgtgtc tgggcaatcc aggacctggg
        70         80         90        100        110        120
ggttacggcg ctattttacg ctatcgcgga cgcgagaaaa cctttagcgc tggctacacc
       130        140        150        160        170        180
cgcaccacca acaaccgtat ggagttgatg gccgctattg tcgcgctgga ggcgttaaaa
       190        200        210        220        230        240
gaacattgcg aagtcatttt gagtaccgac agccagtatg tccgccaggg tatcacccag
       250        260        270        280        290        300
tggatccata actggaaaaa acgtggctgg aaaaccgcag acaaaaaacc agtaaaaaat
       310        320        330        340        350        360
gtcgatctct ggcaacgtct tgatgctgca ttggggcagc atcaaatcaa atgggaatgg
       370        380        390        400        410        420
gttaaaggcc atgccggaca cccggaaaac gaacgctgtg atgaactggc tcgtgccgcg
       430        440        450        460
gcgatgaatc ccacactgga agatacaggc taccaagttg aagtttaa 

Last modification date: Oct. 2, 2011