Mus musculus L. (mouse) [MMU]

FULL NAME: DNA mismatch repair protein Msh6


DESCRIPTION:
Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair (By similarity).

STRUCTURE SIMILARITY:
Contains 1 PWWP domain.
Belongs to the DNA mismatch repair mutS family.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).


RELATED PATHWAY(S):
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MSRQSTLYSF FPKSPALGDT KKAAAEASRQ GAAASGASAS RGGDAAWSEA EPGSRSAAVS
        70         80         90        100        110        120
ASSPEAKDLN GGLRRASSSA QAVPPSSCDF SPGDLVWAKM EGYPWWPCLV YNHPFDGTFI
       130        140        150        160        170        180
RKKGKSVRVH VQFFDDSPTR GWVSKRMLKP YTGSKSKEAQ KGGHFYSSKS EILRAMQRAD
       190        200        210        220        230        240
EALSKDTAER LQLAVCDEPS EPEEEEETEV HEAYLSDKSE EDNYNESEEE AQPSVQGPRR
       250        260        270        280        290        300
SSRQVKKRRV ISDSESDIGG SDVEFKPDTK QEGSSDDASS GVGDSDSEDL GTFGKGAPKR
       310        320        330        340        350        360
KRAMVAQGGL RRKSLKKETG SAKRATPILS ETKSTLSAFS APQNSESQTH VSGGGNDSSG
       370        380        390        400        410        420
PTVWYHETLE WLKPEKRRDE HRRRPDHPEF NPTTLYVPEE FLNSCTPGMR KWWQLKSQNF
       430        440        450        460        470        480
DLVIFYKVGK FYELYHMDAV IGVSELGLIF MKGNWAHSGF PEIAFGRFSD SLVQKGYKVA
       490        500        510        520        530        540
RVEQTETPEM MEARCRKMAH VSKFDRVVRR EICRIITKGT QTYSVLDGDP SENYSRYLLS
       550        560        570        580        590        600
LKEKEEETSG HTRVYGVCFV DTSLGKFFIG QFSDDRHCSR FRTLVAHYPP VQILFEKGNL
       610        620        630        640        650        660
STETKTVLKG SLSSCLQEGL IPGSQFWDAT KTLRTLLEGG YFTGNGDSST VLPLVLKGMT
       670        680        690        700        710        720
SESDSVGLTP GEESELALSA LGGIVFYLKK CLIDQELLSM ANFEEYFPLD SDTVSTVKPG
       730        740        750        760        770        780
AVFTKASQRM VLDAVTLNNL EIFLNGTNGS TEGTLLERLD TCHTPFGKRL LKQWLCAPLC
       790        800        810        820        830        840
SPSAISDRLD AVEDLMAVPD KVTEVADLLK KLPDLERLLS KIHNVGSPLK SQNHPDSRAI
       850        860        870        880        890        900
MYEETTYSKK KIIDFLSALE GFKVMCKVSG LLEEVAGGFT SKTLKQVVTL QSKSPKGRFP
       910        920        930        940        950        960
DLTAELQRWD TAFDHEKARK TGLITPKAGF DSDYDQALAD IRENEQSLLE YLDKQRSRLG
       970        980        990       1000       1010       1020
CKSIVYWGIG RNRYQLEIPE NFATRNLPEE YELKSTKKGC KRYWTKTIEK KLANLINAEE
      1030       1040       1050       1060       1070       1080
RRDTSLKDCM RRLFCNFDKN HKDWQSAVEC IAVLDVLLCL ANYSQGGDGP MCRPEIVLPG
      1090       1100       1110       1120       1130       1140
EDTHPFLEFK GSRHPCITKT FFGDDFIPND ILIGCEEEAE EHGKAYCVLV TGPNMGGKST
      1150       1160       1170       1180       1190       1200
LIRQAGLLAV MAQLGCYVPA EKCRLTPVDR VFTRLGASDR IMSGESTFFV ELSETASILR
      1210       1220       1230       1240       1250       1260
HATAHSLVLV DELGRGTATF DGTAIANAVV KELAETIKCR TLFSTHYHSL VEDYSKSVCV
      1270       1280       1290       1300       1310       1320
RLGHMACMVE NECEDPSQET ITFLYKFIKG ACPKSYGFNA ARLANLPEEV IQKGHRKARE
      1330       1340       1350
FERMNQSLQL FREVCLATEK PTINGEAIHR LLALINGL  

Encoded by Msh6 gene

FULL NAME: mutS homolog 6 (E. coli)


OTHER NAME(S):
AU044881
AW550279
GTBP
Gtmbp


Nucleic acid sequence

        10         20         30         40         50         60
atgtcccgac aaagcacctt gtacagcttc ttccccaagt ccccggcact cggcgacacc
        70         80         90        100        110        120
aagaaggccg cggccgaggc ctcacgccaa ggtgccgctg cctctggagc ctccgcttcc
       130        140        150        160        170        180
cgcggcgggg atgcggcctg gagcgaggct gaacccgggt cccggtccgc ggcggtatcc
       190        200        210        220        230        240
gcctcgtcgc cggaggcaaa ggatctcaac ggagggctgc ggagggcctc ctcctccgcg
       250        260        270        280        290        300
caggccgtgc ctcccagttc ttgtgacttc tcaccaggtg atttggtttg ggctaagatg
       310        320        330        340        350        360
gaaggttacc cctggtggcc ttgcctagtt tataatcatc cctttgatgg aacgttcatc
       370        380        390        400        410        420
cggaagaaag ggaaatctgt ccgtgttcat gtacagttct ttgatgacag cccaacaagg
       430        440        450        460        470        480
ggctgggtta gcaaaaggat gttaaagcca tatacaggtt caaagtcaaa ggaagcccaa
       490        500        510        520        530        540
aagggaggtc atttctacag ttcaaagtct gagatactca gagcaatgca acgtgcagat
       550        560        570        580        590        600
gaggccttaa gtaaagacac ggctgagagg ctgcagctgg cagtgtgtga tgagccttca
       610        620        630        640        650        660
gagccggagg aggaggagga gacagaggtg catgaggctt atttatctga taagagtgaa
       670        680        690        700        710        720
gaggataatt acaacgagag tgaagaagaa gcacagccca gtgttcaggg gcctaggcga
       730        740        750        760        770        780
agcagccggc aagtcaagaa gcgacgggtc atctcagact cggagagtga cattggtggc
       790        800        810        820        830        840
tctgatgtag aattcaagcc agacactaag caggaaggaa gcagtgatga cgcgagcagt
       850        860        870        880        890        900
ggagttgggg acagcgatag tgaggaccta ggcacctttg gcaaaggcgc tccaaagcgg
       910        920        930        940        950        960
aagagagcca tggttgctca gggtggactt agaaggaaaa gtctgaagaa ggaaacaggc
       970        980        990       1000       1010       1020
tcagccaaac gagcaactcc cattctctca gaaaccaaga gtaccttgag cgctttttct
      1030       1040       1050       1060       1070       1080
gcccctcaga attctgaatc ccagacccac gtcagtggag gaggtaacga cagtagtgga
      1090       1100       1110       1120       1130       1140
cccactgttt ggtatcacga aactttagaa tggcttaagc cagaaaagag aagagatgag
      1150       1160       1170       1180       1190       1200
cacaggagac gccccgatca ccctgaattt aaccccacca cactgtatgt gcctgaagag
      1210       1220       1230       1240       1250       1260
ttcctcaatt cttgtacccc agggatgagg aagtggtggc agctcaagtc tcagaacttt
      1270       1280       1290       1300       1310       1320
gacctcgtca tcttttataa ggtggggaag ttttatgagt tgtatcacat ggatgctgtt
      1330       1340       1350       1360       1370       1380
attggagtca gtgagctggg gctgatattc atgaagggca actgggccca ttctggtttt
      1390       1400       1410       1420       1430       1440
ccagagattg cgtttggccg gttctcggat tccttggtgc agaagggcta taaggtagca
      1450       1460       1470       1480       1490       1500
cgagtggagc agaccgagac tccagaaatg atggaggcgc gatgtcggaa aatggcacac
      1510       1520       1530       1540       1550       1560
gtgtccaagt ttgatagagt ggtgagaagg gagatttgca ggatcattac caagggcaca
      1570       1580       1590       1600       1610       1620
cagacctaca gtgtgctgga tggtgatccc tctgagaact acagtaggta tcttcttagc
      1630       1640       1650       1660       1670       1680
ctcaaagaga aggaggaaga gacttctggt cacactcgtg tgtatggtgt ttgctttgtg
      1690       1700       1710       1720       1730       1740
gacacttcac tgggcaagtt tttcatcggc cagttttcgg atgaccgcca ctgttccaga
      1750       1760       1770       1780       1790       1800
tttaggacgc tagtggctca ctatcctcca gtacaaattt tgttcgagaa aggaaatctc
      1810       1820       1830       1840       1850       1860
tcaacagaga ccaaaactgt cctaaagggt tcactgtcat cttgtcttca ggaaggtctc
      1870       1880       1890       1900       1910       1920
ataccaggtt cccaattttg ggatgccact aagaccttgc gaacactcct tgaaggaggg
      1930       1940       1950       1960       1970       1980
tactttactg gaaatggaga cagtagtaca gtgctgcctc tggtgcttaa aggaatgacc
      1990       2000       2010       2020       2030       2040
tcggagtcag attctgttgg gttgacacca ggcgaggaga gtgaattggc tctctctgct
      2050       2060       2070       2080       2090       2100
ctaggtggta ttgttttcta cctcaaaaaa tgcctgattg atcaggagct tctgtcaatg
      2110       2120       2130       2140       2150       2160
gctaattttg aagagtattt ccctttggat tctgacacgg tcagcacagt aaaacctggg
      2170       2180       2190       2200       2210       2220
gctgtcttta ctaaagccag tcagcggatg gtgctagacg cagtgacatt aaacaacctg
      2230       2240       2250       2260       2270       2280
gagattttcc tgaatgggac aaatggctct actgaaggga ccctgctaga gagattggat
      2290       2300       2310       2320       2330       2340
acttgccata cgccctttgg caagcggctc ttaaaacagt ggctctgtgc ccccctctgc
      2350       2360       2370       2380       2390       2400
agcccttctg ccatcagtga ccgtctagat gcggtggaag atctgatggc cgtgcctgac
      2410       2420       2430       2440       2450       2460
aaggtcactg aagttgcaga cctcttaaag aagctgccag accttgagag gctactgagt
      2470       2480       2490       2500       2510       2520
aagattcata atgttgggtc tcccctgaag agccagaacc atccagacag cagggctata
      2530       2540       2550       2560       2570       2580
atgtacgaag aaacgacata cagcaaaaag aagatcattg attttctctc tgctctagaa
      2590       2600       2610       2620       2630       2640
ggattcaaag taatgtgtaa agtctcaggg cttctggagg aggtcgcggg tggttttacg
      2650       2660       2670       2680       2690       2700
tccaaaaccc tcaagcaggt tgttactctg cagtcaaaaa gtcctaaagg ccgctttcct
      2710       2720       2730       2740       2750       2760
gacctgactg cagaactgca gcgatgggac acagcctttg accatgagaa ggctcggaag
      2770       2780       2790       2800       2810       2820
accggactca tcactccaaa agcagggttt gactctgatt atgaccaagc acttgctgac
      2830       2840       2850       2860       2870       2880
atcagagaga atgaacagag cctcctggag tacttagaca aacagcgcag tcggcttggc
      2890       2900       2910       2920       2930       2940
tgtaagagca tagtctactg gggaatcggt aggaaccgtt accagttaga gattccagag
      2950       2960       2970       2980       2990       3000
aatttcgcta cccgtaatct acctgaagaa tatgagctga aatctactaa aaaaggctgt
      3010       3020       3030       3040       3050       3060
aaacgatact ggaccaaaac gattgagaag aaattagcta atcttattaa tgctgaagaa
      3070       3080       3090       3100       3110       3120
cgcagggaca catccttaaa ggactgcatg cggcgcttgt tctgtaactt cgacaagaac
      3130       3140       3150       3160       3170       3180
cacaaggact ggcagtctgc tgtagagtgc attgcagtgt tggatgtctt actgtgcctg
      3190       3200       3210       3220       3230       3240
gctaactaca gtcaaggagg agatggtcct atgtgtcgcc cagaaattgt gttaccagga
      3250       3260       3270       3280       3290       3300
gaagacacac atcccttctt agagtttaaa gggtcacgac atccctgcat tacaaagact
      3310       3320       3330       3340       3350       3360
ttttttggag acgattttat tcctaacgac attctaatag gctgcgagga agaagcagag
      3370       3380       3390       3400       3410       3420
gaacatggca aagcctattg tgtgcttgtg actggaccaa atatgggggg caagtctaca
      3430       3440       3450       3460       3470       3480
ctcataagac aggctggtct gttggctgtg atggcccagc tgggttgtta cgtacctgct
      3490       3500       3510       3520       3530       3540
gagaagtgca ggctcacacc agttgacaga gtgtttacta ggcttggtgc ctcagataga
      3550       3560       3570       3580       3590       3600
ataatgtcag gagaaagcac attttttgtt gagttgagtg aaactgctag catactcagg
      3610       3620       3630       3640       3650       3660
catgcaacag cacattcttt ggtacttgtg gatgaattag gaagaggcac cgcaactttt
      3670       3680       3690       3700       3710       3720
gatgggacag cgatagccaa tgcggttgtt aaagaacttg ctgaaaccat aaagtgtcga
      3730       3740       3750       3760       3770       3780
acactgtttt ctacacacta tcattcatta gtagaagatt attctaaaag tgtttgtgtg
      3790       3800       3810       3820       3830       3840
cgcctaggac atatggcatg catggtagaa aatgaatgtg aggaccccag ccaggagact
      3850       3860       3870       3880       3890       3900
attactttcc tctataaatt cattaaggga gcttgtccta agagctatgg ctttaatgca
      3910       3920       3930       3940       3950       3960
gcaaggcttg ctaatctccc ggaggaggtt attcagaagg gacacagaaa ggcaagagaa
      3970       3980       3990       4000       4010       4020
tttgagagga tgaatcagtc attacagcta tttcgggaag tttgtctggc tactgaaaag
      4030       4040       4050       4060       4070
ccgactataa acggtgaagc tatccatagg ctgctggcct tgattaacgg attgtag

Last modification date: Oct. 2, 2011